Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACEQ85_RS09540 Genome accession   NZ_CP168966
Coordinates   2009154..2009795 (+) Length   213 a.a.
NCBI ID   WP_033848908.1    Uniprot ID   A0A013TY26
Organism   Acinetobacter pittii strain LS-AP     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2004154..2014795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEQ85_RS09520 (ACEQ85_09520) - 2004159..2004491 (+) 333 WP_068563962.1 hypothetical protein -
  ACEQ85_RS09525 (ACEQ85_09525) rrmA 2004494..2005318 (+) 825 WP_373882520.1 putative RNA methyltransferase -
  ACEQ85_RS09530 (ACEQ85_09530) ponA 2005373..2007934 (-) 2562 WP_373882521.1 penicillin-binding protein PBP1a -
  ACEQ85_RS09535 (ACEQ85_09535) pilM 2008096..2009154 (+) 1059 WP_373882522.1 pilus assembly protein PilM Machinery gene
  ACEQ85_RS09540 (ACEQ85_09540) pilN 2009154..2009795 (+) 642 WP_033848908.1 PilN domain-containing protein Machinery gene
  ACEQ85_RS09545 (ACEQ85_09545) pilO 2009792..2010544 (+) 753 WP_060464993.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACEQ85_RS09550 (ACEQ85_09550) pilP 2010555..2011082 (+) 528 WP_068563956.1 pilus assembly protein PilP Machinery gene
  ACEQ85_RS09555 (ACEQ85_09555) pilQ 2011145..2013271 (+) 2127 WP_068563954.1 type IV pilus secretin PilQ Machinery gene
  ACEQ85_RS09560 (ACEQ85_09560) aroK 2013283..2013852 (+) 570 WP_002116987.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 23887.39 Da        Isoelectric Point: 6.4518

>NTDB_id=1048989 ACEQ85_RS09540 WP_033848908.1 2009154..2009795(+) (pilN) [Acinetobacter pittii strain LS-AP]
MATINLLPWRDGLREQRKKQFIILCFGVVVLGITTVFAGWFYLNQKLNDQEQANQLIISTNQNLDQQLKTLNGLQEQRDA
IIERMKLIQGLQSQRPVVVRLVDELVRVTPAAMYLTKFSRTGDKFTIEGKAESPNTVAELLRNLEASPWYRNAFMSSFLA
TEEKKDKAVSSLLPRVEDSYGSFVVTVDVGEMGVTTTDDTANTPLGTDKGVAK

Nucleotide


Download         Length: 642 bp        

>NTDB_id=1048989 ACEQ85_RS09540 WP_033848908.1 2009154..2009795(+) (pilN) [Acinetobacter pittii strain LS-AP]
ATGGCAACAATTAATTTACTCCCTTGGCGTGATGGGCTTAGGGAGCAGCGTAAAAAACAATTTATTATTTTATGTTTTGG
GGTCGTTGTTTTGGGGATAACCACTGTATTTGCAGGGTGGTTTTATTTGAATCAAAAATTAAATGATCAAGAACAAGCAA
ATCAGCTCATCATCAGTACAAACCAGAATCTAGATCAACAACTTAAAACCTTAAATGGCTTGCAAGAACAGCGGGACGCA
ATTATTGAGCGTATGAAGCTCATTCAAGGTTTGCAAAGCCAGCGTCCGGTCGTTGTACGTTTGGTGGATGAATTAGTACG
TGTGACCCCGGCAGCCATGTATTTGACTAAATTCAGCCGAACCGGAGATAAATTTACGATTGAAGGTAAAGCGGAAAGTC
CAAATACAGTTGCAGAGTTATTACGTAATCTCGAAGCATCGCCATGGTATCGCAACGCTTTTATGAGTTCTTTCTTGGCG
ACCGAAGAGAAAAAAGATAAGGCTGTCAGTTCTTTGCTTCCTAGAGTTGAAGATAGCTATGGCAGTTTTGTTGTTACTGT
TGATGTAGGGGAAATGGGGGTGACCACAACAGATGATACCGCCAATACGCCTTTAGGTACGGATAAGGGGGTGGCAAAAT
GA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A013TY26

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Acinetobacter baumannii D1279779

88.732

100

0.887
  comN Acinetobacter baylyi ADP1

71.707

96.244

0.69

Multiple sequence alignment