Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACEQ38_RS03075 Genome accession   NZ_CP168747
Coordinates   640423..642150 (-) Length   575 a.a.
NCBI ID   WP_078222783.1    Uniprot ID   -
Organism   Ralstonia syzygii subsp. celebesensis strain NCPPB 3727     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 635423..647150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEQ38_RS03045 (ACEQ38_03045) - 636043..636501 (+) 459 WP_048817320.1 NUDIX domain-containing protein -
  ACEQ38_RS03050 (ACEQ38_03050) - 636498..636713 (-) 216 WP_013211401.1 DNA gyrase inhibitor YacG -
  ACEQ38_RS03055 (ACEQ38_03055) zapD 636725..637483 (-) 759 WP_013211402.1 cell division protein ZapD -
  ACEQ38_RS03060 (ACEQ38_03060) coaE 637601..638215 (-) 615 WP_078222780.1 dephospho-CoA kinase -
  ACEQ38_RS03065 (ACEQ38_03065) pilD 638228..639109 (-) 882 WP_078222781.1 A24 family peptidase Machinery gene
  ACEQ38_RS03070 (ACEQ38_03070) - 639112..640377 (-) 1266 WP_078222782.1 type II secretion system F family protein -
  ACEQ38_RS03075 (ACEQ38_03075) pilF 640423..642150 (-) 1728 WP_078222783.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEQ38_RS03080 (ACEQ38_03080) - 642398..643675 (-) 1278 WP_013211407.1 HlyC/CorC family transporter -
  ACEQ38_RS03090 (ACEQ38_03090) ispB 643954..644922 (-) 969 WP_078222784.1 octaprenyl diphosphate synthase -
  ACEQ38_RS03095 (ACEQ38_03095) rplU 645201..645512 (+) 312 WP_003271629.1 50S ribosomal protein L21 -
  ACEQ38_RS03100 (ACEQ38_03100) rpmA 645550..645810 (+) 261 WP_004633021.1 50S ribosomal protein L27 -
  ACEQ38_RS03105 (ACEQ38_03105) obgE 645998..647098 (+) 1101 WP_013211409.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63642.39 Da        Isoelectric Point: 7.4490

>NTDB_id=1045462 ACEQ38_RS03075 WP_078222783.1 640423..642150(-) (pilF) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3727]
MTLGLALAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDAVPAVLSTNKHF
QQLRLLPLGRRDNRIIVATSDPSDPKPIEELRQKMNVAIETVIVEHDKLVRQLSLANEAPTEIKSLIPKQEATQIEYDPG
AAAAVRRTTTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILAQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKETIERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLTNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPRETL
LEAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILSHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1045462 ACEQ38_RS03075 WP_078222783.1 640423..642150(-) (pilF) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3727]
ATGACTCTCGGACTCGCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCACCCAGCTTGAACAAAGCGCACGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCGCACGATCTCGCCGTCTTCGTGGCGGGCA
AGTACCAGATGCCGCTGCTGGACCTGGCGCAGTACAAGCTCGATGCCGTGCCGGCCGTGCTGTCGACCAACAAGCATTTC
CAGCAATTGCGCCTGTTGCCGCTGGGCCGGCGCGACAACCGCATCATCGTTGCCACCTCGGACCCGAGCGACCCCAAGCC
GATCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCGCCAACGAAGCGCCGACCGAGATCAAGTCGCTCATCCCCAAGCAGGAAGCCACGCAGATCGAATACGACCCGGGC
GCAGCCGCTGCAGTGCGGCGTACCACGACCGACGGCATCGACGATGCGCCGGTGGTGCGTTTCCTGCAAAAGCTGTTTAC
CGAAGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGTATCGACG
GCATCCTCGCGCAGGTCGCGCAGCCACCGCTGGATATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATCTCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACGGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGACGATCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCCACCGAGCGGCTCGATATCGACCAGCTAGGCTATGAGCCCGAGCAAAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAAACGGTCTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAAGCGGGGCTGACCTTCGCCGCGGCGCTGAAATCGTTCCTGCGGCAGGATCCGGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGATGCGCCGACCACGCTCACGCGGCTGACGAACATGGGCGTGGCACCGTTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCATGCAAGAAGCCGGGCCCGCTGCCCCGGGAAACACTG
CTGGAGGCCGGCTTCAAGGAAGCCGACCTGGACGGCTCGTGGCAGCCGTACCATCCAGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCAGCCACG
GCACGGCACTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCTGGCCTGCGGAAAGTGAAA
CAGGGGCTGACTTCCCTTGAGGAAGTGCTGGCCACGACGAACCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.519

93.913

0.503
  pilB Acinetobacter baumannii D1279779

51.15

98.261

0.503
  pilB Acinetobacter baylyi ADP1

50.089

97.913

0.49
  pilB Legionella pneumophila strain ERS1305867

46.585

99.304

0.463
  pilB Vibrio cholerae strain A1552

44.784

96.696

0.433
  pilB Vibrio campbellii strain DS40M4

44.834

94.261

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

44.649

94.261

0.421
  pilF Thermus thermophilus HB27

37.699

98.261

0.37

Multiple sequence alignment