Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEPM3_RS14730 Genome accession   NZ_CP168727
Coordinates   3093804..3095531 (+) Length   575 a.a.
NCBI ID   WP_011002731.1    Uniprot ID   A0A223GQM6
Organism   Ralstonia pseudosolanacearum strain RUN6499     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3088804..3100531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM3_RS14700 (ACEPM3_14700) obgE 3088858..3089958 (-) 1101 WP_011002727.1 GTPase ObgE -
  ACEPM3_RS14705 (ACEPM3_14705) rpmA 3090135..3090395 (-) 261 WP_003271630.1 50S ribosomal protein L27 -
  ACEPM3_RS14710 (ACEPM3_14710) rplU 3090433..3090744 (-) 312 WP_011002728.1 50S ribosomal protein L21 -
  ACEPM3_RS14715 (ACEPM3_14715) ispB 3091023..3091991 (+) 969 WP_011002729.1 octaprenyl diphosphate synthase -
  ACEPM3_RS14725 (ACEPM3_14725) - 3092279..3093556 (+) 1278 WP_011002730.1 HlyC/CorC family transporter -
  ACEPM3_RS14730 (ACEPM3_14730) pilB 3093804..3095531 (+) 1728 WP_011002731.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEPM3_RS14735 (ACEPM3_14735) pilC 3095576..3096841 (+) 1266 WP_011002732.1 type II secretion system F family protein Machinery gene
  ACEPM3_RS14740 (ACEPM3_14740) - 3096844..3097725 (+) 882 WP_029240316.1 A24 family peptidase -
  ACEPM3_RS14745 (ACEPM3_14745) coaE 3097738..3098352 (+) 615 WP_011002734.1 dephospho-CoA kinase -
  ACEPM3_RS14750 (ACEPM3_14750) zapD 3098470..3099228 (+) 759 WP_011002735.1 cell division protein ZapD -
  ACEPM3_RS14755 (ACEPM3_14755) yacG 3099239..3099454 (+) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  ACEPM3_RS14760 (ACEPM3_14760) - 3099447..3099908 (-) 462 WP_011002737.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63770.60 Da        Isoelectric Point: 7.1546

>NTDB_id=1045331 ACEPM3_RS14730 WP_011002731.1 3093804..3095531(+) (pilB) [Ralstonia pseudosolanacearum strain RUN6499]
MTLGLSLAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1045331 ACEPM3_RS14730 WP_011002731.1 3093804..3095531(+) (pilB) [Ralstonia pseudosolanacearum strain RUN6499]
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCACCCAGCTAGAACAAAGCGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCGCAATACAAGCTCGACACCGTGCCGCCTGTGCTGTCGGCCAACAGGCATTTT
CAGCAGTTGCGCCTGTTGCCGCTGGGCCGGCGTGACAACCGCATCATCGTGGCCACTTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCTCCAACGAGGCACCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCACGCAGATCGAATACGACCCGGGT
GCGGCCGCGGCGGTCCGCCGCACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATATCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGATGGTCGAGCGCGCTATCGACTTCCGGGTGTCGACGCTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAGTCCTTTCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCGCCATTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGTCCGCTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAGGAAGCAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCCGGCCTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-131)

Type II/IV secretion system protein (T2SSE)

(184-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A223GQM6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.06

98.435

0.503
  pilF Neisseria gonorrhoeae MS11

52.93

94.957

0.503
  pilB Acinetobacter baylyi ADP1

49.554

97.565

0.483
  pilB Legionella pneumophila strain ERS1305867

47.163

98.087

0.463
  pilB Vibrio cholerae strain A1552

44.821

97.391

0.437
  pilB Vibrio parahaemolyticus RIMD 2210633

45.203

94.261

0.426
  pilB Vibrio campbellii strain DS40M4

44.526

95.304

0.424
  pilF Thermus thermophilus HB27

38.256

97.739

0.374

Multiple sequence alignment