Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEN9H_RS24810 Genome accession   NZ_CP168562
Coordinates   5687493..5689226 (+) Length   577 a.a.
NCBI ID   WP_056122895.1    Uniprot ID   A0A7X3K8C0
Organism   Massilia cellulosiltytica strain CT11-92     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5682493..5694226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9H_RS24790 (ACEN9H_24795) - 5682585..5684156 (+) 1572 WP_413672447.1 methyl-accepting chemotaxis protein -
  ACEN9H_RS24795 (ACEN9H_24800) - 5684348..5685031 (+) 684 WP_156403646.1 hypothetical protein -
  ACEN9H_RS24800 (ACEN9H_24805) - 5685036..5685851 (+) 816 WP_413672448.1 slipin family protein -
  ACEN9H_RS24805 (ACEN9H_24810) - 5685938..5687245 (+) 1308 WP_413340974.1 HlyC/CorC family transporter -
  ACEN9H_RS24810 (ACEN9H_24815) pilB 5687493..5689226 (+) 1734 WP_056122895.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEN9H_RS24815 (ACEN9H_24820) pilC 5689236..5690474 (+) 1239 WP_056122892.1 type II secretion system F family protein Machinery gene
  ACEN9H_RS24820 (ACEN9H_24825) - 5691005..5691913 (-) 909 WP_413672449.1 LysR substrate-binding domain-containing protein -
  ACEN9H_RS24825 (ACEN9H_24830) - 5692008..5693186 (+) 1179 WP_413672450.1 SGNH/GDSL hydrolase family protein -
  ACEN9H_RS24830 (ACEN9H_24835) - 5693191..5694027 (+) 837 WP_413672451.1 AraC family transcriptional regulator -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 61953.28 Da        Isoelectric Point: 5.2763

>NTDB_id=1044562 ACEN9H_RS24810 WP_056122895.1 5687493..5689226(+) (pilB) [Massilia cellulosiltytica strain CT11-92]
MAAVLPNTTPGTALPGLARALVQAGRLTLPQADTLQKKASADKAGFIDTLLGSSSIDAKSLAVFCAETFGYPLFDLGTFS
HDALPVNAIDPKLMQAQRVVALARRGNKLSVAVSDPTNTQALDQIKFQSESTVEPVIVAHDTLLALLANIAKSAEQDLSE
LAGEEGDIEFADEEPQTQAAEAAAAAAEVEDAPIVRFLNKVLMDAITLGASDIHFEPYEKQYRIRLRVDGVLRDHLAPPL
SIRDKLVSRIKVLAKLDISEKRVPQDGRMRLVLSPTKTIDFRVSTLPTLFGEKTVMRILDATQAQMGIDALGYDPDQKAL
LIDAITRPYGMVLVTGPTGSGKTVSLYSCLNLLNKPGINISTAEDPAEINLPGVNQVNVNERAGLTFPVALKAFLRQDPD
IIMVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCTCKQPLDIGRD
TLKAAGFRDTDFDGDWKPYGPVGCERCLGSGYKGRVGIYQIMPITPAIEALILGHGNAMQIAAQAEAEGVNSLRRSGLLK
VKQGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1044562 ACEN9H_RS24810 WP_056122895.1 5687493..5689226(+) (pilB) [Massilia cellulosiltytica strain CT11-92]
ATGGCAGCAGTCCTTCCCAACACCACCCCCGGCACGGCGTTGCCCGGGCTGGCGCGTGCCCTGGTGCAGGCCGGACGGCT
GACCTTGCCTCAAGCGGACACCTTGCAAAAGAAGGCGTCCGCCGACAAGGCCGGTTTCATCGACACCTTGCTCGGCAGCA
GCTCCATCGATGCGAAATCCCTGGCAGTTTTCTGCGCCGAGACCTTCGGCTATCCCCTGTTCGACCTGGGCACGTTCAGC
CACGACGCCCTGCCCGTCAACGCGATCGACCCGAAGCTGATGCAGGCCCAGCGCGTCGTCGCGCTGGCACGCAGGGGCAA
CAAATTGTCGGTGGCGGTGTCCGACCCGACGAATACGCAGGCCCTCGACCAGATCAAGTTCCAGAGCGAATCGACCGTCG
AACCGGTGATCGTCGCGCACGACACCCTGCTGGCGCTGCTGGCGAACATCGCCAAGAGCGCCGAACAGGACTTGAGCGAA
CTGGCCGGCGAGGAAGGCGACATCGAATTCGCCGACGAAGAACCGCAGACGCAGGCGGCGGAAGCGGCAGCCGCCGCGGC
GGAAGTCGAGGACGCGCCCATCGTGCGCTTCCTCAACAAGGTGTTGATGGATGCAATTACCTTGGGCGCGTCGGACATCC
ACTTCGAGCCCTACGAAAAGCAGTACCGCATCCGCCTGCGCGTGGACGGCGTACTGCGCGACCATCTCGCCCCGCCGCTG
TCGATCCGCGACAAGCTCGTCTCCCGCATCAAGGTGCTGGCCAAGCTCGACATCTCGGAAAAGCGCGTGCCGCAGGACGG
CCGCATGCGCCTCGTGCTGTCGCCCACGAAGACGATCGATTTCCGCGTCAGCACCTTGCCGACCCTGTTCGGCGAAAAAA
CGGTCATGCGTATCCTCGACGCGACGCAGGCCCAGATGGGCATCGACGCGCTCGGCTACGACCCCGACCAGAAAGCGTTG
CTGATCGACGCCATCACGCGCCCGTACGGCATGGTGCTCGTAACGGGCCCGACGGGTTCGGGCAAAACCGTGTCGCTGTA
CAGCTGCCTGAACCTGTTGAACAAGCCGGGGATCAATATCTCCACGGCGGAAGACCCGGCCGAGATCAACCTGCCCGGCG
TGAACCAGGTCAACGTCAACGAAAGGGCGGGCCTCACGTTCCCCGTCGCGCTGAAAGCCTTCCTGCGCCAGGATCCGGAC
ATCATCATGGTCGGCGAGATCCGCGACCTGGAAACGGCCGACATCGCCATCAAGGCGGCGCAGACCGGCCACATGGTGTT
TTCCACCCTGCACACGAACGATGCGCCGTCGACCTTGACGCGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCCT
CGTCCGTCATCCTGATCACCGCGCAGCGCCTCGCGCGCCGCCTGTGCACGTGCAAGCAGCCACTCGACATCGGCCGCGAC
ACGCTGAAGGCCGCGGGCTTCCGCGACACCGACTTCGACGGCGACTGGAAGCCCTACGGCCCCGTCGGCTGCGAGCGCTG
CCTGGGCTCCGGCTACAAGGGCCGCGTGGGCATCTACCAGATCATGCCGATCACGCCCGCGATCGAAGCGTTGATTCTTG
GGCACGGCAACGCGATGCAGATCGCCGCCCAGGCCGAGGCCGAGGGCGTGAACTCGCTGCGCCGCTCCGGCCTGCTGAAA
GTGAAACAAGGCCTCACCAGTCTCGAAGAAGTGCTCGGCTGCACGAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-155)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X3K8C0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.167

99.827

0.541
  pilB Acinetobacter baylyi ADP1

54.196

99.133

0.537
  pilF Neisseria gonorrhoeae MS11

53.427

98.614

0.527
  pilB Legionella pneumophila strain ERS1305867

49.204

97.92

0.482
  pilB Vibrio cholerae strain A1552

45.979

99.133

0.456
  pilB Vibrio campbellii strain DS40M4

45.375

99.307

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

45.922

97.747

0.449
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.298

98.787

0.388
  pilF Thermus thermophilus HB27

40.112

92.894

0.373

Multiple sequence alignment