Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   ACD269_RS09855 Genome accession   NZ_CP168260
Coordinates   1902112..1902573 (-) Length   153 a.a.
NCBI ID   WP_000250520.1    Uniprot ID   C1CA95
Organism   Streptococcus pneumoniae strain FC2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1897112..1907573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACD269_RS09835 (ACD269_09835) comYH 1898338..1899291 (-) 954 WP_000345132.1 class I SAM-dependent methyltransferase Machinery gene
  ACD269_RS09840 (ACD269_09840) - 1899637..1900439 (+) 803 Protein_1913 IS5 family transposase -
  ACD269_RS09845 (ACD269_09845) - 1900590..1901066 (+) 477 WP_000777686.1 GNAT family N-acetyltransferase -
  ACD269_RS09850 (ACD269_09850) comGG/cglG 1901721..1902134 (-) 414 WP_000265615.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACD269_RS09855 (ACD269_09855) comGF/cglF 1902112..1902573 (-) 462 WP_000250520.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACD269_RS09860 (ACD269_09860) comGE/cglE 1902536..1902838 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACD269_RS09865 (ACD269_09865) comGD/cglD 1902801..1903235 (-) 435 WP_033606693.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACD269_RS09870 (ACD269_09870) comGC/cglC 1903198..1903521 (-) 324 WP_000738628.1 comG operon protein ComGC Machinery gene
  ACD269_RS09875 (ACD269_09875) comGB/cglB 1903523..1904539 (-) 1017 WP_078141518.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACD269_RS09880 (ACD269_09880) comGA/cglA/cilD 1904487..1905428 (-) 942 WP_000249572.1 competence type IV pilus ATPase ComGA Machinery gene
  ACD269_RS09885 (ACD269_09885) - 1905504..1905869 (-) 366 WP_000286415.1 DUF1033 family protein -
  ACD269_RS09890 (ACD269_09890) - 1906020..1907078 (-) 1059 WP_000649465.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17905.50 Da        Isoelectric Point: 8.0031

>NTDB_id=1043529 ACD269_RS09855 WP_000250520.1 1902112..1902573(-) (comGF/cglF) [Streptococcus pneumoniae strain FC2]
MVQNSCWQSKSHKVKAFTLLESLIALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKEIAMGKSKADDFRKTNARGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=1043529 ACD269_RS09855 WP_000250520.1 1902112..1902573(-) (comGF/cglF) [Streptococcus pneumoniae strain FC2]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGATTGCCCTGAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGAGATTGCTATGGGTAAGTCTAAGGCAGATGATTTCCGTAAAACGAATGCTCG
TGGTCGAGGTTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C1CA95

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae D39

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae R6

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae TIGR4

93.464

100

0.935
  comGF/cglF Streptococcus mitis NCTC 12261

90.85

100

0.909
  comGF/cglF Streptococcus mitis SK321

90.85

100

0.909

Multiple sequence alignment