Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACEN2W_RS12120 Genome accession   NZ_CP168246
Coordinates   717706..719433 (-) Length   575 a.a.
NCBI ID   WP_014616069.1    Uniprot ID   F6FY28
Organism   Ralstonia solanacearum strain 071-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 712706..724433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN2W_RS12090 (ACEN2W_12085) - 713323..713784 (+) 462 WP_014616065.1 NUDIX domain-containing protein -
  ACEN2W_RS12095 (ACEN2W_12090) - 713777..713992 (-) 216 WP_003266032.1 DNA gyrase inhibitor YacG -
  ACEN2W_RS12100 (ACEN2W_12095) zapD 714009..714767 (-) 759 WP_003266031.1 cell division protein ZapD -
  ACEN2W_RS12105 (ACEN2W_12100) coaE 714885..715499 (-) 615 WP_014616066.1 dephospho-CoA kinase -
  ACEN2W_RS12110 (ACEN2W_12105) pilD 715512..716393 (-) 882 WP_014616067.1 A24 family peptidase Machinery gene
  ACEN2W_RS12115 (ACEN2W_12110) pilG 716396..717661 (-) 1266 WP_014616068.1 type II secretion system F family protein Machinery gene
  ACEN2W_RS12120 (ACEN2W_12115) pilF 717706..719433 (-) 1728 WP_014616069.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEN2W_RS12125 (ACEN2W_12120) - 719681..720958 (-) 1278 WP_014616070.1 HlyC/CorC family transporter -
  ACEN2W_RS12135 (ACEN2W_12130) ispB 721233..722201 (-) 969 WP_003271627.1 octaprenyl diphosphate synthase -
  ACEN2W_RS12140 (ACEN2W_12135) rplU 722480..722791 (+) 312 WP_003271629.1 50S ribosomal protein L21 -
  ACEN2W_RS12145 (ACEN2W_12140) rpmA 722829..723089 (+) 261 WP_003271630.1 50S ribosomal protein L27 -
  ACEN2W_RS12150 (ACEN2W_12145) obgE 723288..724388 (+) 1101 WP_014616072.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63814.59 Da        Isoelectric Point: 6.9139

>NTDB_id=1043430 ACEN2W_RS12120 WP_014616069.1 717706..719433(-) (pilF) [Ralstonia solanacearum strain 071-2]
MTLGLALTQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPAVLSTNKHF
QQLRLLPLGRRDNRIIVATSDPSDPKPIEELRQKMNVAIETVIVEHDKLVRQLSLANEAPPEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKETVDRAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEEDLDGSWQPYHPVGCEACNSSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1043430 ACEN2W_RS12120 WP_014616069.1 717706..719433(-) (pilF) [Ralstonia solanacearum strain 071-2]
ATGACTCTCGGACTCGCACTTACGCAGAGCCGGCGAATTGCGCCGACATTACTCACTCAGCTAGAGCAGAGCGCGCGCGA
AAAGCGCACGCAGTTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCGCACGACCTCGCCGTCTTCGTGGCGGGCA
AGTACCAGATGCCGCTGCTGGACCTGGCGCAGTACAAGCTCGACACCGTGCCGGCCGTGCTGTCGACCAACAAGCATTTC
CAGCAACTGCGCCTGCTGCCGCTGGGCCGGCGCGACAACCGCATCATCGTTGCCACCTCGGACCCGAGCGACCCGAAGCC
GATCGAAGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAACTGA
GCCTCGCCAACGAAGCGCCGCCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCACCCAGATCGAATACGACCCGGGC
GCAGCCGCGGCCGTGCGGCGTACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTCAC
CGAGGCGCTGCTGCGCGGCACGTCCGACTTGCACTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTTGAACAAGTCGCGCAACCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTATCGCGCCTG
GACATCTCGGAAAAACGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCACCGTTCCGAAGGACAAGAACGACAAGGA
CAACAAGGAGACGGTCGATCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAACGCCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGATCCGGCCGAAATTCAACTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAATCGTTCCTGCGCCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGATCTCGAAACCGCCGACATCTCCATCAAGGCCGCACAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTCACACGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCCTCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGCCCGCTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAAGAAGAAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACAG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACCGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAGGGCGTGCTCTCGCTGCGTGACTCCGGCCTGAGAAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGCTGGCCACGACGAACCAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)

Type II/IV secretion system protein (T2SSE)

(184-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6FY28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.211

94.783

0.504
  pilB Acinetobacter baumannii D1279779

51.15

98.261

0.503
  pilB Acinetobacter baylyi ADP1

49.912

98.261

0.49
  pilB Legionella pneumophila strain ERS1305867

46.235

99.304

0.459
  pilB Vibrio cholerae strain A1552

44.464

97.391

0.433
  pilB Vibrio campbellii strain DS40M4

44.526

95.304

0.424
  pilB Vibrio parahaemolyticus RIMD 2210633

44.834

94.261

0.423
  pilF Thermus thermophilus HB27

37.321

97.391

0.363

Multiple sequence alignment