Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACEN2U_RS14705 Genome accession   NZ_CP168242
Coordinates   2944940..2945461 (-) Length   173 a.a.
NCBI ID   WP_014617873.1    Uniprot ID   F6G3Z1
Organism   Ralstonia solanacearum strain 095-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2921310..2945461 2944940..2945461 within 0


Gene organization within MGE regions


Location: 2921310..2945461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN2U_RS14605 (ACEN2U_14605) - 2921310..2922071 (+) 762 WP_014617860.1 tyrosine-type recombinase/integrase -
  ACEN2U_RS14610 (ACEN2U_14610) - 2922119..2923329 (+) 1211 WP_346825000.1 IS3 family transposase -
  ACEN2U_RS14615 (ACEN2U_14615) - 2923295..2924977 (-) 1683 WP_014617868.1 AAA family ATPase -
  ACEN2U_RS14620 (ACEN2U_14620) - 2924977..2925660 (-) 684 WP_124994723.1 hypothetical protein -
  ACEN2U_RS14625 (ACEN2U_14625) - 2925772..2926536 (-) 765 WP_042567705.1 hypothetical protein -
  ACEN2U_RS14630 (ACEN2U_14630) - 2926590..2927156 (-) 567 WP_042567706.1 recombinase family protein -
  ACEN2U_RS14635 (ACEN2U_14635) - 2927410..2928072 (+) 663 WP_014617864.1 hypothetical protein -
  ACEN2U_RS14640 (ACEN2U_14640) - 2928075..2929277 (+) 1203 WP_014617863.1 ImmA/IrrE family metallo-endopeptidase -
  ACEN2U_RS14645 (ACEN2U_14645) - 2929894..2934885 (-) 4992 WP_014617862.1 type ISP restriction/modification enzyme -
  ACEN2U_RS14650 (ACEN2U_14650) - 2935048..2935626 (-) 579 WP_124994724.1 hypothetical protein -
  ACEN2U_RS14660 (ACEN2U_14660) - 2936944..2937666 (-) 723 WP_086002895.1 HNH endonuclease -
  ACEN2U_RS14670 (ACEN2U_14670) - 2939001..2939240 (-) 240 WP_014617869.1 hypothetical protein -
  ACEN2U_RS14675 (ACEN2U_14675) istA 2939639..2940655 (+) 1017 WP_039548595.1 IS21 family transposase -
  ACEN2U_RS14680 (ACEN2U_14680) istB 2940652..2941440 (+) 789 WP_014615828.1 IS21-like element ISRso19 family helper ATPase IstB -
  ACEN2U_RS14685 (ACEN2U_14685) - 2941492..2941785 (-) 294 WP_346824833.1 hypothetical protein -
  ACEN2U_RS14690 (ACEN2U_14690) moaC 2942167..2942268 (+) 102 Protein_2707 cyclic pyranopterin monophosphate synthase MoaC -
  ACEN2U_RS14695 (ACEN2U_14695) - 2942345..2944081 (-) 1737 WP_014617871.1 Wzy polymerase domain-containing protein -
  ACEN2U_RS14700 (ACEN2U_14700) pilA 2944166..2944672 (-) 507 WP_014617872.1 pilin Machinery gene
  ACEN2U_RS14705 (ACEN2U_14705) pilA 2944940..2945461 (-) 522 WP_014617873.1 pilin Machinery gene

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17727.44 Da        Isoelectric Point: 10.2418

>NTDB_id=1043420 ACEN2U_RS14705 WP_014617873.1 2944940..2945461(-) (pilA) [Ralstonia solanacearum strain 095-3]
MASKHSRNRRRGQRGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVTEGLSLAAQAKALVVENAANSQSSLALGSASL
PASKNVSALNIDASTGEITVVYASAVTTGGANTLVLTPYSGSSSALANLRAGSAPPAMVLWVCAAAGKSMPVTTVTQNTT
PTLPPKYAPAECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=1043420 ACEN2U_RS14705 WP_014617873.1 2944940..2945461(-) (pilA) [Ralstonia solanacearum strain 095-3]
ATGGCATCGAAGCACAGCAGGAACAGGCGGCGCGGTCAGCGCGGCTTTACGTTGATCGAGTTGATGATCGTGGTGGCGAT
CATCGGGATACTGGCGGCGATTGCGATTCCGGCGTACCAGGACTACACGATCCGGGCGCGCGTGACGGAAGGGCTGTCGC
TGGCTGCACAGGCCAAGGCGCTGGTGGTTGAGAATGCCGCCAACTCCCAGAGCAGTTTGGCGCTCGGCTCCGCATCATTG
CCGGCGAGCAAGAATGTCAGTGCTCTGAATATCGATGCGTCTACGGGTGAGATTACGGTGGTCTATGCGTCAGCTGTCAC
GACCGGAGGCGCGAACACGCTGGTTTTGACTCCATACTCGGGAAGCTCGTCAGCACTTGCTAACTTGCGCGCTGGCTCTG
CGCCGCCGGCAATGGTGTTGTGGGTGTGCGCTGCCGCCGGAAAGTCGATGCCGGTCACCACCGTCACCCAGAACACGACG
CCTACGTTGCCGCCGAAATATGCGCCTGCGGAATGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(45-173)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6G3Z1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

54.545

100

0.555
  pilA2 Legionella pneumophila str. Paris

47.273

95.376

0.451
  pilA2 Legionella pneumophila strain ERS1305867

46.667

95.376

0.445
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.462

100

0.434
  comP Acinetobacter baylyi ADP1

41.615

93.064

0.387

Multiple sequence alignment