Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACEN2U_RS04225 Genome accession   NZ_CP168242
Coordinates   717666..719393 (-) Length   575 a.a.
NCBI ID   WP_014616069.1    Uniprot ID   F6FY28
Organism   Ralstonia solanacearum strain 095-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 712666..724393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN2U_RS04195 (ACEN2U_04195) - 713283..713744 (+) 462 WP_014616065.1 NUDIX domain-containing protein -
  ACEN2U_RS04200 (ACEN2U_04200) - 713737..713952 (-) 216 WP_003266032.1 DNA gyrase inhibitor YacG -
  ACEN2U_RS04205 (ACEN2U_04205) zapD 713969..714727 (-) 759 WP_003266031.1 cell division protein ZapD -
  ACEN2U_RS04210 (ACEN2U_04210) coaE 714845..715459 (-) 615 WP_014616066.1 dephospho-CoA kinase -
  ACEN2U_RS04215 (ACEN2U_04215) pilD 715472..716353 (-) 882 WP_014616067.1 A24 family peptidase Machinery gene
  ACEN2U_RS04220 (ACEN2U_04220) pilG 716356..717621 (-) 1266 WP_014616068.1 type II secretion system F family protein Machinery gene
  ACEN2U_RS04225 (ACEN2U_04225) pilF 717666..719393 (-) 1728 WP_014616069.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEN2U_RS04230 (ACEN2U_04230) - 719641..720918 (-) 1278 WP_014616070.1 HlyC/CorC family transporter -
  ACEN2U_RS04240 (ACEN2U_04240) ispB 721193..722161 (-) 969 WP_003271627.1 octaprenyl diphosphate synthase -
  ACEN2U_RS04245 (ACEN2U_04245) rplU 722440..722751 (+) 312 WP_003271629.1 50S ribosomal protein L21 -
  ACEN2U_RS04250 (ACEN2U_04250) rpmA 722789..723049 (+) 261 WP_003271630.1 50S ribosomal protein L27 -
  ACEN2U_RS04255 (ACEN2U_04255) obgE 723248..724348 (+) 1101 WP_014616072.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63814.59 Da        Isoelectric Point: 6.9139

>NTDB_id=1043409 ACEN2U_RS04225 WP_014616069.1 717666..719393(-) (pilF) [Ralstonia solanacearum strain 095-3]
MTLGLALTQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPAVLSTNKHF
QQLRLLPLGRRDNRIIVATSDPSDPKPIEELRQKMNVAIETVIVEHDKLVRQLSLANEAPPEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKETVDRAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEEDLDGSWQPYHPVGCEACNSSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1043409 ACEN2U_RS04225 WP_014616069.1 717666..719393(-) (pilF) [Ralstonia solanacearum strain 095-3]
ATGACTCTCGGACTCGCACTTACGCAGAGCCGGCGAATTGCGCCGACATTACTCACTCAGCTAGAGCAGAGCGCGCGCGA
AAAGCGCACGCAGTTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCGCACGACCTCGCCGTCTTCGTGGCGGGCA
AGTACCAGATGCCGCTGCTGGACCTGGCGCAGTACAAGCTCGACACCGTGCCGGCCGTGCTGTCGACCAACAAGCATTTC
CAGCAACTGCGCCTGCTGCCGCTGGGCCGGCGCGACAACCGCATCATCGTTGCCACCTCGGACCCGAGCGACCCGAAGCC
GATCGAAGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAACTGA
GCCTCGCCAACGAAGCGCCGCCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCACCCAGATCGAATACGACCCGGGC
GCAGCCGCGGCCGTGCGGCGTACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTCAC
CGAGGCGCTGCTGCGCGGCACGTCCGACTTGCACTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTTGAACAAGTCGCGCAACCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTATCGCGCCTG
GACATCTCGGAAAAACGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCACCGTTCCGAAGGACAAGAACGACAAGGA
CAACAAGGAGACGGTCGATCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAACGCCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGATCCGGCCGAAATTCAACTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAATCGTTCCTGCGCCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGATCTCGAAACCGCCGACATCTCCATCAAGGCCGCACAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTCACACGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCCTCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGCCCGCTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAAGAAGAAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACAG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACCGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAGGGCGTGCTCTCGCTGCGTGACTCCGGCCTGAGAAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGCTGGCCACGACGAACCAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)

Type II/IV secretion system protein (T2SSE)

(184-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.211

94.783

0.504
  pilB Acinetobacter baumannii D1279779

51.15

98.261

0.503
  pilB Acinetobacter baylyi ADP1

49.912

98.261

0.49
  pilB Legionella pneumophila strain ERS1305867

46.235

99.304

0.459
  pilB Vibrio cholerae strain A1552

44.464

97.391

0.433
  pilB Vibrio campbellii strain DS40M4

44.526

95.304

0.424
  pilB Vibrio parahaemolyticus RIMD 2210633

44.834

94.261

0.423
  pilF Thermus thermophilus HB27

37.321

97.391

0.363

Multiple sequence alignment