Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB9L16_RS21905 Genome accession   NZ_CP168138
Coordinates   4850892..4852592 (+) Length   566 a.a.
NCBI ID   WP_056790439.1    Uniprot ID   A0AAQ3G636
Organism   Pseudomonas sp. LW8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4845892..4857592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9L16_RS21885 (AB9L16_21885) - 4846082..4849033 (+) 2952 WP_252868296.1 DUF748 domain-containing protein -
  AB9L16_RS21890 (AB9L16_21890) - 4849030..4849329 (+) 300 WP_095181833.1 DUF2845 domain-containing protein -
  AB9L16_RS21895 (AB9L16_21895) - 4849594..4849947 (-) 354 WP_071173629.1 BON domain-containing protein -
  AB9L16_RS21900 (AB9L16_21900) pilA/pilAI 4850243..4850665 (-) 423 WP_252876912.1 pilin Machinery gene
  AB9L16_RS21905 (AB9L16_21905) pilB 4850892..4852592 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB9L16_RS21910 (AB9L16_21910) pilC 4852595..4853812 (+) 1218 WP_150638505.1 type II secretion system F family protein Machinery gene
  AB9L16_RS21915 (AB9L16_21915) pilD 4853814..4854686 (+) 873 WP_252876914.1 A24 family peptidase Machinery gene
  AB9L16_RS21920 (AB9L16_21920) coaE 4854683..4855306 (+) 624 WP_252876915.1 dephospho-CoA kinase -
  AB9L16_RS21925 (AB9L16_21925) yacG 4855303..4855503 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  AB9L16_RS21930 (AB9L16_21930) - 4855511..4855726 (-) 216 WP_016773148.1 hypothetical protein -
  AB9L16_RS21935 (AB9L16_21935) - 4855792..4856481 (-) 690 WP_041477814.1 energy-coupling factor ABC transporter permease -
  AB9L16_RS21940 (AB9L16_21940) - 4856672..4857298 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62620.67 Da        Isoelectric Point: 6.1319

>NTDB_id=1042506 AB9L16_RS21905 WP_056790439.1 4850892..4852592(+) (pilB) [Pseudomonas sp. LW8]
MNDIALSGLAKQLVQAELLTETSARQSWAQAQRNRVSLVNYLVHNKLVNSRQIAEIASEHFGMALLDLHCLDKETQPKGL
VSEKLVRQHCALPLWRRGNKLFVGLSDPSNQQAISDIQFSTGLSTEAILVEDDKLSDAIDKFFDSHASGLEEMADVDLDG
LDVESIDDNKQDVIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRMRTDGMLREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEAEQKALYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCAHCKRPLEIPHETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFADLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1042506 AB9L16_RS21905 WP_056790439.1 4850892..4852592(+) (pilB) [Pseudomonas sp. LW8]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAACTGCTCACGGAAACAAGCGCCCGGCAATC
CTGGGCGCAGGCCCAGCGCAATCGCGTGTCGCTGGTCAATTACCTGGTGCACAACAAACTGGTCAACAGCCGGCAAATTG
CCGAGATCGCCTCGGAACATTTCGGCATGGCCCTGCTCGATCTGCATTGCCTCGACAAAGAGACTCAGCCCAAGGGCCTG
GTCAGCGAAAAACTGGTTCGTCAGCACTGCGCCCTGCCCTTGTGGCGGCGCGGCAACAAACTGTTCGTGGGCCTTTCCGA
CCCAAGCAATCAGCAGGCGATCAGCGACATCCAGTTCAGCACCGGCCTGAGCACCGAAGCGATTCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGACAAGTTCTTCGACAGCCATGCCTCGGGCCTGGAGGAAATGGCCGATGTCGATCTCGACGGC
CTCGACGTCGAGTCCATTGATGACAACAAACAGGACGTGATCGGCGGCCTCGATGCCGACGACGCCCCGGTCGTACGTTT
TGTGCACAAGATGCTGCTCGATGCGATCAAAAGCGGCTCTTCCGACCTGCATTTCGAACCCTACGAAAAAAACTATCGGG
TGCGCATGCGCACCGACGGCATGCTGCGCGAGGTCGCCAAGCCGCCGATCCAGTTGGCCGGGCGCATTGCCGCGCGCTTG
AAGGTAATGGCCAGTCTGGATATCTCGGAACGGCGCAAACCTCAGGACGGCCGCATCAAAATGCGCCTGTCGAAAAGCAA
ATCCATCGATTTTCGCGTCAACACCCTGCCGACTCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGTG
CACAAATGGGCATCGATGCGCTGGGCTACGAGGCCGAACAAAAAGCCTTGTACCTGGCCGCGCTGAAACAGCCGCAAGGG
ATGATTCTGGTCACCGGCCCCACTGGCTCCGGTAAAACCGTGTCGCTGTACACCGGGCTGAACATCCTCAACACCGTCGA
CATCAATATTTCCACCGCCGAAGATCCGGTGGAAATCAACATGGAAGGTATCAATCAGGTCAACGTCAATCCCCGCCAAG
GGCTGGACTTTGCCCAAGCGCTGCGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCAATCAAGGCTGCGCAGACCGGCCACCTGGTGCTGTCGACCCTGCACACCAATAGCGCAGCGGA
AACCCTGACACGCCTGCACAACATGGGCATTCCCGGTTTCAACATCGCCACCTCGGTCAGCCTGATCATCGCCCAGCGTC
TGGCGCGCAAGTTGTGCGCACACTGCAAGCGGCCGCTGGAGATCCCGCACGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGTCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAATGGCGGTTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTAAAGAACACACCGGACCTGCAACGGTTGATCATGGCCGAGGGCAATTCGCTGGAAATCGATATTCAGA
TGCGCCGCGACGGTTTCGCCGATCTGCGCACTTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.699

99.823

0.576
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

56.537

100

0.565
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.446

99.117

0.5
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.209

100

0.495
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.837

94.17

0.366

Multiple sequence alignment