Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACDW38_RS23450 Genome accession   NZ_CP167986
Coordinates   5064746..5066449 (+) Length   567 a.a.
NCBI ID   WP_023100230.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 0805d0     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5051543..5075152 5064746..5066449 within 0


Gene organization within MGE regions


Location: 5051543..5075152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDW38_RS23370 (ACDW38_23370) - 5051543..5052727 (+) 1185 WP_174214394.1 tyrosine-type recombinase/integrase -
  ACDW38_RS23375 (ACDW38_23375) - 5053044..5053340 (+) 297 WP_109721881.1 AlpA family transcriptional regulator -
  ACDW38_RS23380 (ACDW38_23380) - 5053344..5055422 (+) 2079 WP_174214395.1 AAA family ATPase -
  ACDW38_RS23385 (ACDW38_23385) - 5055869..5056387 (+) 519 WP_174214396.1 hypothetical protein -
  ACDW38_RS23390 (ACDW38_23390) - 5056380..5056625 (+) 246 WP_174214397.1 hypothetical protein -
  ACDW38_RS23395 (ACDW38_23395) - 5057275..5058000 (+) 726 WP_174214398.1 hypothetical protein -
  ACDW38_RS23400 (ACDW38_23400) - 5057993..5058274 (+) 282 WP_217438146.1 hypothetical protein -
  ACDW38_RS23405 (ACDW38_23405) - 5058497..5059006 (+) 510 WP_174214399.1 hypothetical protein -
  ACDW38_RS23410 (ACDW38_23410) - 5059009..5060121 (-) 1113 WP_174214400.1 restriction endonuclease, SacI family -
  ACDW38_RS23415 (ACDW38_23415) - 5060229..5061326 (+) 1098 WP_174214401.1 DNA cytosine methyltransferase -
  ACDW38_RS23420 (ACDW38_23420) - 5061530..5061994 (+) 465 WP_174214402.1 hypothetical protein -
  ACDW38_RS23425 (ACDW38_23425) - 5062008..5062277 (+) 270 WP_174214403.1 hypothetical protein -
  ACDW38_RS23430 (ACDW38_23430) - 5062290..5062802 (+) 513 WP_174214404.1 hypothetical protein -
  ACDW38_RS23440 (ACDW38_23440) tfpZ 5063181..5063918 (-) 738 WP_023110409.1 TfpX/TfpZ family type IV pilin accessory protein -
  ACDW38_RS23445 (ACDW38_23445) pilE 5063983..5064519 (-) 537 WP_025921208.1 pilin Machinery gene
  ACDW38_RS23450 (ACDW38_23450) pilB 5064746..5066449 (+) 1704 WP_023100230.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACDW38_RS23455 (ACDW38_23455) - 5066453..5067669 (+) 1217 Protein_4635 type II secretion system F family protein -
  ACDW38_RS23460 (ACDW38_23460) pilD 5067672..5068544 (+) 873 WP_017148550.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACDW38_RS23465 (ACDW38_23465) coaE 5068541..5069152 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  ACDW38_RS23470 (ACDW38_23470) yacG 5069149..5069349 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  ACDW38_RS23475 (ACDW38_23475) - 5069386..5069595 (-) 210 WP_003094660.1 hypothetical protein -
  ACDW38_RS23480 (ACDW38_23480) - 5069701..5070390 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  ACDW38_RS23485 (ACDW38_23485) - 5070387..5070857 (-) 471 WP_023103507.1 hypothetical protein -
  ACDW38_RS23490 (ACDW38_23490) - 5070854..5071279 (-) 426 WP_016562341.1 GNAT family N-acetyltransferase -
  ACDW38_RS23495 (ACDW38_23495) - 5071412..5072041 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  ACDW38_RS23500 (ACDW38_23500) - 5072038..5072487 (+) 450 WP_003161439.1 MOSC domain-containing protein -
  ACDW38_RS23505 (ACDW38_23505) - 5072513..5072683 (+) 171 WP_003094672.1 DUF3094 family protein -
  ACDW38_RS23510 (ACDW38_23510) - 5072747..5074054 (+) 1308 WP_003112837.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62580.51 Da        Isoelectric Point: 5.5586

>NTDB_id=1040859 ACDW38_RS23450 WP_023100230.1 5064746..5066449(+) (pilB) [Pseudomonas aeruginosa strain 0805d0]
MNDTIQLSGLARQLVLHELLDEKAAQQAQLQAQRNKLSLVTYLVQSKLVKGQALTELAADQFGIAYCDLNSLDRDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRASGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1040859 ACDW38_RS23450 WP_023100230.1 5064746..5066449(+) (pilB) [Pseudomonas aeruginosa strain 0805d0]
ATGAACGATACCATCCAGCTCAGCGGCCTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAAGCAGCTCAACA
GGCCCAGTTGCAAGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGACAGGCCT
TGACCGAACTGGCGGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGATCGGGACAGCCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGGGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGCCTGGATGACGTGGATCTTGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTATTGGATGCGATCAGAGGCGGCTCCTCTGATCTGCATTTCGAGCCCTATGAAAAGATTTACC
GTGTACGTTTCCGTACCGATGGCATGCTCCATGAGGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTAATGGCAGGCCTGGACATTTCCGAACGGCGTAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTTAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGTATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTAGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGACCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAAC
AGGCCCGCAAAGAAGGCTTCAACGATCTACGCGCTTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.07

100

0.584
  pilB Acinetobacter baylyi ADP1

57.447

99.471

0.571
  pilB Legionella pneumophila strain ERS1305867

55.282

100

0.554
  pilF Neisseria gonorrhoeae MS11

50.444

99.295

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.763

95.062

0.492
  pilB Vibrio cholerae strain A1552

50.545

97.002

0.49
  pilB Vibrio campbellii strain DS40M4

50.832

95.414

0.485
  pilF Thermus thermophilus HB27

38.584

99.647

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.189

93.474

0.376

Multiple sequence alignment