Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACDW35_RS25285 Genome accession   NZ_CP167983
Coordinates   5441727..5443430 (+) Length   567 a.a.
NCBI ID   WP_003141354.1    Uniprot ID   A0A0H2ZH73
Organism   Pseudomonas aeruginosa strain 0102d1_7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5430487..5440895 5441727..5443430 flank 832


Gene organization within MGE regions


Location: 5430487..5443430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDW35_RS25200 (ACDW35_25200) - 5430487..5431701 (+) 1215 WP_023082006.1 site-specific integrase -
  ACDW35_RS25205 (ACDW35_25205) - 5431703..5432245 (-) 543 WP_123809552.1 hypothetical protein -
  ACDW35_RS25210 (ACDW35_25210) - 5432389..5432589 (+) 201 WP_023082007.1 helix-turn-helix domain-containing protein -
  ACDW35_RS25215 (ACDW35_25215) - 5432586..5433002 (+) 417 WP_034054964.1 hypothetical protein -
  ACDW35_RS25220 (ACDW35_25220) - 5432995..5433228 (+) 234 WP_031653969.1 hypothetical protein -
  ACDW35_RS25225 (ACDW35_25225) - 5433225..5433473 (+) 249 WP_031653970.1 hypothetical protein -
  ACDW35_RS25230 (ACDW35_25230) - 5433674..5434111 (+) 438 WP_305956564.1 hypothetical protein -
  ACDW35_RS25235 (ACDW35_25235) - 5434150..5434617 (+) 468 WP_031629313.1 hypothetical protein -
  ACDW35_RS25240 (ACDW35_25240) - 5434624..5435751 (+) 1128 WP_073662618.1 AAA family ATPase -
  ACDW35_RS25245 (ACDW35_25245) - 5435751..5436044 (+) 294 WP_073662617.1 hypothetical protein -
  ACDW35_RS25250 (ACDW35_25250) - 5436041..5436271 (+) 231 WP_034055169.1 helix-turn-helix domain-containing protein -
  ACDW35_RS25255 (ACDW35_25255) - 5436386..5436598 (+) 213 WP_023109351.1 hypothetical protein -
  ACDW35_RS25260 (ACDW35_25260) - 5436601..5438442 (+) 1842 WP_034055171.1 ClpP-like prohead protease/major capsid protein fusion protein -
  ACDW35_RS25265 (ACDW35_25265) - 5439712..5439954 (+) 243 WP_023109353.1 hypothetical protein -
  ACDW35_RS25275 (ACDW35_25275) - 5440197..5440895 (-) 699 WP_016254081.1 hypothetical protein -
  ACDW35_RS25280 (ACDW35_25280) - 5440959..5441498 (-) 540 WP_003141352.1 pilin -
  ACDW35_RS25285 (ACDW35_25285) pilB 5441727..5443430 (+) 1704 WP_003141354.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62610.54 Da        Isoelectric Point: 5.5603

>NTDB_id=1040755 ACDW35_RS25285 WP_003141354.1 5441727..5443430(+) (pilB) [Pseudomonas aeruginosa strain 0102d1_7]
MNDTIQLSGLARQLVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFP
EDKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1040755 ACDW35_RS25285 WP_003141354.1 5441727..5443430(+) (pilB) [Pseudomonas aeruginosa strain 0102d1_7]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTAATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGCTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCAGTGGTGCG
TTTCGTCAACAAAATGTTGTTGGATGCGATCAGAGGCGGCTCCTCCGACCTGCATTTCGAGCCCTATGAAAAGATTTACC
GCGTACGTTTCCGTACCGATGGCATGCTCCATGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTGATGGCAGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCTA
GCGCGCAGATGGGCATCGACGCCCTGGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAATCTGATCATCGCCCAGC
GCCTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCG
GAAGACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZH73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.58
  pilB Acinetobacter baylyi ADP1

57.27

99.471

0.57
  pilB Legionella pneumophila strain ERS1305867

55.106

100

0.552
  pilF Neisseria gonorrhoeae MS11

50.444

99.295

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.948

95.062

0.494
  pilB Vibrio cholerae strain A1552

50.182

97.002

0.487
  pilB Vibrio campbellii strain DS40M4

50.832

95.414

0.485
  pilF Thermus thermophilus HB27

38.584

99.647

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.189

93.474

0.376

Multiple sequence alignment