NTDB

Overview

Name	ruvB	Type	Machinery gene
Locus tag	KJP57_RS15210	Genome accession	NZ_OX419573
Coordinates	2870765..2871769 (-)	Length	334 a.a.
NCBI ID	WP_003229718.1	Uniprot ID	O32055
Organism	Bacillus subtilis isolate NRS6085
Function	homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2865765..2876769

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KJP57_RS15180 (NRS6085_15160)	yrbG	2866739..2867395 (-)	657	WP_128738641.1	DUF421 domain-containing protein	-
KJP57_RS15185 (NRS6085_15165)	-	2867593..2867982 (+)	390	WP_031315243.1	TIGR04086 family membrane protein	-
KJP57_RS15190 (NRS6085_15170)	yajC	2868039..2868305 (-)	267	WP_004398708.1	preprotein translocase subunit YajC	-
KJP57_RS15195 (NRS6085_15175)	tgt	2868342..2869487 (-)	1146	WP_003229725.1	tRNA guanosine(34) transglycosylase Tgt	-
KJP57_RS15200 (NRS6085_15180)	queA	2869514..2870542 (-)	1029	WP_003229723.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
KJP57_RS15205 (NRS6085_15185)	yrzS	2870572..2870772 (-)	201	WP_003222669.1	DUF2905 domain-containing protein	-
KJP57_RS15210 (NRS6085_15190)	ruvB	2870765..2871769 (-)	1005	WP_003229718.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
KJP57_RS15215 (NRS6085_15195)	ruvA	2871780..2872385 (-)	606	WP_046381240.1	Holliday junction branch migration protein RuvA	Machinery gene
KJP57_RS15220 (NRS6085_15200)	bofC	2872524..2873036 (-)	513	WP_003229715.1	sporulation cell-cell signaling protein BofC	-
KJP57_RS15225 (NRS6085_15205)	csbX	2873084..2874391 (-)	1308	WP_046381241.1	MFS transporter	-
KJP57_RS15230 (NRS6085_15210)	iolG	2874462..2875487 (-)	1026	WP_046381242.1	inositol 2-dehydrogenase	-
KJP57_RS15235 (NRS6085_15215)	yrzF	2875725..2876372 (+)	648	WP_003246159.1	phosphotransferase	-
KJP57_RS15240 (NRS6085_15220)	-	2876418..2876540 (-)	123	WP_003229707.1	hypothetical protein	-

Sequence

Protein

Download Length: 334 a.a. Molecular weight: 37467.78 Da Isoelectric Point: 5.1631

                                >NTDB_id=1040641 KJP57_RS15210 WP_003229718.1 2870765..2871769(-) (ruvB) [Bacillus subtilis isolate NRS6085]

MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE

LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT

RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI

TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP

AVYHHFQMEAPRYD

Nucleotide

Download Length: 1005 bp

                                >NTDB_id=1040641 KJP57_RS15210 WP_003229718.1 2870765..2871769(-) (ruvB) [Bacillus subtilis isolate NRS6085]

ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC

ACAGTATATCGGACAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG

ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCGATTGTTGCTAATGAAATGGGAGTGGAA

TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT

ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG

ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGGCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACG

AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTAATGTCTCGCCTTGAGTACTATACACAAGAAGAACT

GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT

CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT

ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT

GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA

CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG

GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	100	100	1
ruvB	Streptococcus pneumoniae TIGR4	60.429	97.605	0.59
ruvB	Streptococcus pneumoniae R6	60.429	97.605	0.59
ruvB	Streptococcus pneumoniae D39	60.429	97.605	0.59
ruvB	Synechocystis sp. PCC 6803	57.143	98.503	0.563
ruvB	Helicobacter pylori 26695	52.744	98.204	0.518

Detailed information NTG1040641