Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCP99_RS05990 Genome accession   NZ_CP167849
Coordinates   1385588..1387324 (+) Length   578 a.a.
NCBI ID   WP_071117396.1    Uniprot ID   -
Organism   Xanthomonas sp. NCPPB 3443     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1372260..1388274 1385588..1387324 within 0


Gene organization within MGE regions


Location: 1372260..1388274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCP99_RS05940 (ACCP99_05940) - 1372260..1376303 (-) 4044 WP_372356856.1 RHS repeat-associated core domain-containing protein -
  ACCP99_RS05945 (ACCP99_05945) coaE 1376962..1377573 (-) 612 WP_277572157.1 dephospho-CoA kinase -
  ACCP99_RS05950 (ACCP99_05950) - 1377587..1378450 (-) 864 WP_003491180.1 A24 family peptidase -
  ACCP99_RS05955 (ACCP99_05955) pilC 1378457..1379719 (-) 1263 WP_069288196.1 type II secretion system F family protein Machinery gene
  ACCP99_RS05960 (ACCP99_05960) - 1379830..1381791 (-) 1962 WP_071117393.1 hypothetical protein -
  ACCP99_RS05965 (ACCP99_05965) - 1381858..1382262 (-) 405 WP_372356857.1 pilin -
  ACCP99_RS05970 (ACCP99_05970) - 1382499..1382999 (+) 501 WP_372356858.1 EamA family transporter -
  ACCP99_RS05975 (ACCP99_05975) - 1383004..1383798 (+) 795 WP_005915828.1 glycosyltransferase -
  ACCP99_RS05980 (ACCP99_05980) - 1383813..1384841 (+) 1029 WP_372356859.1 glycosyltransferase -
  ACCP99_RS05985 (ACCP99_05985) - 1384834..1385490 (+) 657 WP_157733690.1 methionine biosynthesis protein MetW -
  ACCP99_RS05990 (ACCP99_05990) pilB 1385588..1387324 (+) 1737 WP_071117396.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62562.59 Da        Isoelectric Point: 5.5986

>NTDB_id=1039828 ACCP99_RS05990 WP_071117396.1 1385588..1387324(+) (pilB) [Xanthomonas sp. NCPPB 3443]
MNSVVTANLAGITGIARRLVQDGALDEGAARAAMDQAATAKVPLPQWFSEKKLVTASQLAAANAVEFGMPLLDVSAFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFIGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1039828 ACCP99_RS05990 WP_071117396.1 1385588..1387324(+) (pilB) [Xanthomonas sp. NCPPB 3443]
ATGAATAGCGTCGTCACAGCTAACTTGGCTGGTATTACGGGAATCGCCCGGCGTCTTGTGCAGGACGGTGCCTTGGATGA
AGGCGCGGCGCGTGCTGCGATGGATCAGGCCGCTACTGCAAAGGTCCCGTTGCCTCAATGGTTCTCGGAGAAAAAGTTGG
TGACAGCTTCGCAGCTGGCGGCCGCCAATGCAGTCGAGTTCGGCATGCCGCTGCTGGATGTATCGGCGTTCGATGCCAAC
CAGAACGCGGTCAAGCTGGTCAGTGAGGAGCTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCATAGGGGTGAGCAACCCGACACAGACTCGCGCGCTGGACGACATCAAGTTCCATACGAACTTAGTGGTCGAGGCGA
TCCTCGTCGACGAAGATCAGATCCGCCGGACGCTCGAGCAGTGGCAGGCCAGCAATGCGTCGTTGGGTTCTTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGCTTGTTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAACGACGAGACACGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCCTGCCTG
GCGTCAATCAGGTACAGCAGAACAACAAGCGTGGCATGACCTTTGCAGCAGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTA
CCTCGTCGGTGACCTTGGTGATCGCGCAACGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCCTTGCTGGCCGAAGGATTCACCCCCGCACAGCTTGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACGGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATG
AAGGCTGCCCACGGGGTGACCAGCCTGGCCGAGATCAATCGCGTGACAAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

55.106

98.27

0.542
  pilB Legionella pneumophila strain ERS1305867

52.548

98.443

0.517
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio cholerae strain A1552

50

95.848

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

46.786

96.886

0.453
  pilB Vibrio campbellii strain DS40M4

45.343

98.443

0.446

Multiple sequence alignment