Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABRP16_RS01095 Genome accession   NZ_CP167825
Coordinates   246245..247969 (-) Length   574 a.a.
NCBI ID   WP_101100949.1    Uniprot ID   -
Organism   Stenotrophomonas lacuserhaii strain WHRI 8086     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 241245..252969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABRP16_RS01070 (ABRP16_001070) ruvX 241642..242109 (-) 468 WP_042614628.1 Holliday junction resolvase RuvX -
  ABRP16_RS01075 (ABRP16_001075) - 242102..242668 (-) 567 WP_182657388.1 YqgE/AlgH family protein -
  ABRP16_RS01080 (ABRP16_001080) - 242745..244538 (+) 1794 WP_349998555.1 DUF4153 domain-containing protein -
  ABRP16_RS01085 (ABRP16_001085) - 244535..245068 (+) 534 WP_101100834.1 DNA-3-methyladenine glycosylase I -
  ABRP16_RS01090 (ABRP16_001090) - 245216..245923 (-) 708 WP_042614624.1 YitT family protein -
  ABRP16_RS01095 (ABRP16_001095) pilB 246245..247969 (-) 1725 WP_101100949.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABRP16_RS01100 (ABRP16_001100) - 248413..248700 (-) 288 WP_349998556.1 hypothetical protein -
  ABRP16_RS01105 (ABRP16_001105) - 248697..249113 (-) 417 WP_349998557.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62486.50 Da        Isoelectric Point: 6.0468

>NTDB_id=1039612 ABRP16_RS01095 WP_101100949.1 246245..247969(-) (pilB) [Stenotrophomonas lacuserhaii strain WHRI 8086]
MNAVATANLVGITGIARRLVQDGALDEAGARDAMAKATAARLPLPQWFAQNKVVTAAQLAAANSLEFGMPLFDVGVFDAN
QSAIKLVSEELLRKHHVLPLFRRGGKLYVGTSDPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWQTSHDSMSSQMGDD
DEGMANLEIGTDDDNSAGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKLN
QRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQEKLFKD
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRRTELPAHAL
LAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEISTIILAGGNALQIAEAAQAIGVNDLRQSALVKASK
GVTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1039612 ABRP16_RS01095 WP_101100949.1 246245..247969(-) (pilB) [Stenotrophomonas lacuserhaii strain WHRI 8086]
ATGAACGCAGTCGCCACCGCAAATCTCGTCGGTATCACCGGTATTGCCCGTCGGCTGGTCCAGGACGGGGCGCTGGATGA
AGCCGGCGCGCGCGATGCGATGGCCAAGGCGACCGCCGCGCGGCTGCCGCTGCCGCAGTGGTTCGCGCAGAACAAGGTCG
TCACCGCCGCCCAGCTGGCCGCCGCCAATTCGCTTGAATTCGGCATGCCGCTGTTCGATGTCGGCGTGTTCGATGCCAAC
CAGAGCGCGATCAAGCTGGTCAGCGAAGAGCTGCTGCGCAAGCACCACGTGCTGCCGCTGTTCAGGCGCGGCGGCAAGCT
GTACGTGGGCACCAGCGACCCCACCCATTCGCTGGATGAAATCAAGTTCCACACCAACCTGGTGGTGGAACCGATCCTGG
TGGATGAAGACCAGATCCGCCGCACGCTGGAGCAATGGCAGACCAGCCACGATTCGATGTCCTCGCAGATGGGCGACGAC
GACGAGGGCATGGCCAACCTGGAAATCGGTACCGACGACGACAACAGCGCCGGTGATTCGGGGATCGACGCCAAGGGCGA
TGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCAAGGGCGCCTCGGATATCCATTTCGAGC
CCTACGAAGACGACTACCGCGTGCGCCTGCGCATCGACGGCCTGTTGAAGATGGTGGCGCGTGCGCCGGTGAAATTGAAC
CAGCGCATCGCCGCGCGCCTGAAGGTCATGGCGCAGCTGGATATCGCTGAAAAGCGCGTGCCGCAGGATGGGCGCATCAA
GCTCAACCTGAGCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCGACCCTGTTCGGTGAAAAGGTGGTGCTGC
GTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAAGCGGACCAGGAGAAGCTGTTCAAGGAC
GCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTTTCGCTGTACACCGCGCT
GGGCATCCTCAACGATGAAACGCGCAATATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTCAACCAGG
TGCAGCAGAACAACAAGCGCGGCATGACCTTCGCTGCGGCACTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATCATG
GTCGGCGAAATCCGCGATCTGGAAACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTCCACGCT
GCATACCAACGATGCACCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCGTACAACATCACCAGTTCGGTGA
CGTTGGTGATCGCCCAGCGGCTGGCACGCCGGCTGTGCAACAACTGCAAGCGCCGCACCGAGCTGCCGGCCCACGCGCTG
CTGGCCGAAGGCTTCACCCAGGCCCAGCTGGATGCGGGTATCGAGCTGTATGAAGCCGTTGGCTGCGATGAATGCACCGA
AGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGATGAGATTTCCACGATCATCCTGGCCGGCGGCA
ATGCGCTGCAGATCGCCGAAGCCGCCCAGGCGATCGGGGTGAACGACCTGCGCCAGTCGGCGCTGGTGAAGGCAAGCAAG
GGCGTCACCAGCCTGGCCGAGATCAATCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.113

100

0.554
  pilB Acinetobacter baylyi ADP1

55.654

98.606

0.549
  pilB Legionella pneumophila strain ERS1305867

52.734

98.78

0.521
  pilF Neisseria gonorrhoeae MS11

48.587

98.606

0.479
  pilB Vibrio cholerae strain A1552

49.451

95.122

0.47
  pilB Vibrio parahaemolyticus RIMD 2210633

46.099

98.258

0.453
  pilB Vibrio campbellii strain DS40M4

45.599

98.955

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

35.737

100

0.388

Multiple sequence alignment