Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACCP95_RS15260 Genome accession   NZ_CP167219
Coordinates   3556482..3558218 (-) Length   578 a.a.
NCBI ID   WP_022558667.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. fici strain NCPPB 3795     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3551482..3563218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCP95_RS15245 (ACCP95_15245) sucC 3551524..3552693 (-) 1170 WP_007965356.1 ADP-forming succinate--CoA ligase subunit beta -
  ACCP95_RS15250 (ACCP95_15250) - 3552925..3554538 (+) 1614 WP_057681814.1 PAS domain-containing sensor histidine kinase -
  ACCP95_RS15255 (ACCP95_15255) pilR 3554806..3556260 (+) 1455 WP_218488478.1 sigma-54 dependent transcriptional regulator Regulator
  ACCP95_RS15260 (ACCP95_15260) pilB 3556482..3558218 (-) 1737 WP_022558667.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACCP95_RS15265 (ACCP95_15265) - 3558282..3558716 (-) 435 WP_078590286.1 pilin -
  ACCP95_RS15270 (ACCP95_15270) pilE 3558794..3559234 (-) 441 WP_078590287.1 pilin Machinery gene
  ACCP95_RS15275 (ACCP95_15275) pilC 3559561..3560817 (+) 1257 WP_057685777.1 type II secretion system F family protein Machinery gene
  ACCP95_RS15280 (ACCP95_15280) - 3560824..3561687 (+) 864 WP_122272419.1 A24 family peptidase -
  ACCP95_RS15285 (ACCP95_15285) coaE 3561701..3562312 (+) 612 WP_007973907.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62578.67 Da        Isoelectric Point: 5.6006

>NTDB_id=1038929 ACCP95_RS15260 WP_022558667.1 3556482..3558218(-) (pilB) [Xanthomonas campestris pv. fici strain NCPPB 3795]
MSVLSTANLVGITGIARRLVQDGALEESAARSAMDQAALAKVPLPQWFSEKKLVAASQLAAANAVEFGMPLLDVSAFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1038929 ACCP95_RS15260 WP_022558667.1 3556482..3558218(-) (pilB) [Xanthomonas campestris pv. fici strain NCPPB 3795]
ATGAGCGTTTTATCGACCGCAAATCTCGTTGGAATCACCGGAATTGCCCGCAGGCTGGTGCAGGATGGCGCATTGGAGGA
AAGCGCGGCTCGAAGCGCGATGGATCAGGCTGCTCTTGCGAAAGTTCCACTGCCTCAGTGGTTCTCGGAGAAAAAGCTTG
TTGCTGCCTCGCAGCTGGCGGCGGCCAATGCAGTCGAGTTCGGCATGCCACTACTGGACGTATCGGCATTCGACGCCAGC
CAGAACGCAGTAAAGCTCGTCAGTGAAGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGTGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAGGCGA
TCCTCGTCGACGAAGATCAGATCCGCCGGACGCTCGAGCAGTGGCAGGCCAGCAATGCGTCGTTGGGTTCTTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGCTTGTTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AAGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGCATCCTCAACGACGAGACACGCAATATCTCCACCGCGGAGGACCCGGTTGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTTGCCGCAGCGTTGCGCTCGTTCCTACGCCAGGACCCA
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATTA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCCTTGCTGGCTGAAGGATTCACCCCCGCACAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACGGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGATGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCCTTGATG
AAGGCTGCCCACGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.959

100

0.561
  pilB Acinetobacter baylyi ADP1

54.75

100

0.548
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilB Vibrio cholerae strain A1552

48.873

99.827

0.488
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

96.886

0.455
  pilB Vibrio campbellii strain DS40M4

45.694

98.443

0.45

Multiple sequence alignment