Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   ACB354_RS00665 Genome accession   NZ_CP167004
Coordinates   104793..105827 (+) Length   344 a.a.
NCBI ID   WP_011184088.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain Isolate 28     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99793..110827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACB354_RS00655 (ACB354_00655) - 103461..103826 (+) 366 WP_002986560.1 DUF1033 family protein -
  ACB354_RS00660 (ACB354_00660) comYA 103919..104857 (+) 939 WP_002986557.1 competence type IV pilus ATPase ComGA Machinery gene
  ACB354_RS00665 (ACB354_00665) comYB 104793..105827 (+) 1035 WP_011184088.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACB354_RS00670 (ACB354_00670) comYC 105829..106155 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  ACB354_RS00675 (ACB354_00675) comGD 106130..106558 (+) 429 WP_011184089.1 competence type IV pilus minor pilin ComGD -
  ACB354_RS00680 (ACB354_00680) comGE 106515..106799 (+) 285 WP_027968839.1 competence type IV pilus minor pilin ComGE -
  ACB354_RS00685 (ACB354_00685) comYF 106792..107226 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACB354_RS00690 (ACB354_00690) comGG 107210..107536 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  ACB354_RS00695 (ACB354_00695) comYH 107634..108587 (+) 954 WP_021340467.1 class I SAM-dependent methyltransferase Machinery gene
  ACB354_RS00700 (ACB354_00700) - 108646..109842 (+) 1197 WP_002986533.1 acetate kinase -
  ACB354_RS00705 (ACB354_00705) - 110029..110337 (+) 309 WP_011017251.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39704.16 Da        Isoelectric Point: 9.7153

>NTDB_id=1037013 ACB354_RS00665 WP_011184088.1 104793..105827(+) (comYB) [Streptococcus pyogenes strain Isolate 28]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGRLLKQYLTSYYAREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1037013 ACB354_RS00665 WP_011184088.1 104793..105827(+) (comYB) [Streptococcus pyogenes strain Isolate 28]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAGACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCCGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCTTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.687

98.547

0.529
  comYB Streptococcus mutans UA159

53.687

98.547

0.529
  comYB Streptococcus gordonii str. Challis substr. CH1

50.588

98.837

0.5
  comGB/cglB Streptococcus mitis NCTC 12261

47.619

97.674

0.465
  comGB/cglB Streptococcus mitis SK321

47.748

96.802

0.462
  comGB/cglB Streptococcus pneumoniae Rx1

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae D39

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae R6

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae TIGR4

45.646

96.802

0.442
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404

Multiple sequence alignment