Detailed information NTG1036291

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB9V61_RS11500
• Genome accession: NZ_CP166925
• Coordinates: 2588074..2589243 (+)
• Length: 389 a.a.
• NCBI ID: WP_011168499.1
• Uniprot ID: Q48JA6
• Organism: Pseudomonas savastanoi pv. phaseolicola strain HB10Y
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2583074..2594243

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB9V61_RS11485 (AB9V61_11485)	-	2583511..2585838 (+)	2328	WP_019741978.1	ATP-dependent DNA helicase	-
AB9V61_RS11490 (AB9V61_11490)	pgm	2585954..2587600 (+)	1647	WP_011168498.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
AB9V61_RS11495 (AB9V61_11495)	-	2587675..2587950 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
AB9V61_RS11500 (AB9V61_11500)	pilU	2588074..2589243 (+)	1170	WP_011168499.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB9V61_RS11505 (AB9V61_11505)	-	2589292..2590869 (-)	1578	WP_011168500.1	ABC transporter ATP-binding protein	-
AB9V61_RS11510 (AB9V61_11510)	-	2590871..2591893 (-)	1023	WP_011168501.1	ABC transporter permease	-
AB9V61_RS11515 (AB9V61_11515)	-	2591893..2592957 (-)	1065	WP_011168502.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42552.70 Da
• Isoelectric Point: 6.0634

>NTDB_id=1036291 AB9V61_RS11500 WP_011168499.1 2588074..2589243(+) (pilU) [Pseudomonas savastanoi pv. phaseolicola strain HB10Y]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMDKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=1036291 AB9V61_RS11500 WP_011168499.1 2588074..2589243(+) (pilU) [Pseudomonas savastanoi pv. phaseolicola strain HB10Y]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGGGCAGTGAAACCCTCAAGCCGGGCGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGATATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGATGTGATCATGGAAAAGCACGGGCTGGTGTTGTTCGTCGGTGCCACCGGTT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAAGTGGGGGTTGATACCCGCAGTTTCCGCGC
GGCGCTGAAGAACACGTTGCGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCCATCTCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTTAGAACCCCGGACGGCAAACGTCGCGCAGCGGTGGAGGTGATGATGGGCACGCCGACCATTCGCGATCTGATTC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGATAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAGGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTTAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGACGCCGCCCCCGGCCCCGACCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGCGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

3D structure

• AlphaFold DB: Q48JA6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.011	93.059	0.54
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.687	98.458	0.391
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362