Detailed information NTG1036247

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AB9V62_RS02295
• Genome accession: NZ_CP166923
• Coordinates: 490302..491366 (+)
• Length: 354 a.a.
• NCBI ID: WP_074855141.1
• Uniprot ID: A0A2S7FHW1
• Organism: Pseudomonas oleovorans strain ERA
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 485302..496366

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB9V62_RS02285 (AB9V62_02285)	-	486351..487619 (+)	1269	WP_074855142.1	malic enzyme-like NAD(P)-binding protein	-
AB9V62_RS02290 (AB9V62_02290)	-	487709..490117 (-)	2409	WP_371254531.1	penicillin-binding protein 1A	-
AB9V62_RS02295 (AB9V62_02295)	pilM	490302..491366 (+)	1065	WP_074855141.1	pilus assembly protein PilM	Machinery gene
AB9V62_RS02300 (AB9V62_02300)	pilN	491366..491938 (+)	573	WP_003459024.1	type 4a pilus biogenesis protein PilN	-
AB9V62_RS02305 (AB9V62_02305)	pilO	491935..492558 (+)	624	WP_003459026.1	type 4a pilus biogenesis protein PilO	-
AB9V62_RS02310 (AB9V62_02310)	pilP	492555..493079 (+)	525	WP_104730118.1	type 4a pilus biogenesis lipoprotein PilP	-
AB9V62_RS02315 (AB9V62_02315)	pilQ	493135..495255 (+)	2121	WP_206408431.1	type IV pilus secretin PilQ	Machinery gene
AB9V62_RS02320 (AB9V62_02320)	aroK	495261..495779 (+)	519	WP_084341405.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37839.28 Da
• Isoelectric Point: 4.4647

>NTDB_id=1036247 AB9V62_RS02295 WP_074855141.1 490302..491366(+) (pilM) [Pseudomonas oleovorans strain ERA]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKTGVKTAAV
AVAGSAVITKIIEMEAGLSEDDLENQLKIEADQYIPYPLEEVAIDFEVQGPAPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLEAQLGGGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1036247 AB9V62_RS02295 WP_074855141.1 490302..491366(+) (pilM) [Pseudomonas oleovorans strain ERA]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACTTCGGTCAAGCTCCTCGAACT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCCTATGCCGTCGAGCCACTTCCGCCCAATGCAGTGGTCGAGAAGAACA
TTGCCGAGTTGGAGGGGGTCGGTCAGGCGCTTTCTCGCGTGTTGGCCAAGGCCAAGACCGGTGTCAAGACGGCCGCGGTT
GCTGTGGCGGGTTCGGCTGTCATCACCAAGATCATCGAAATGGAAGCGGGTCTTTCTGAGGACGACTTGGAAAACCAGCT
GAAGATCGAGGCCGATCAATACATTCCTTACCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGGCCGGCGCCGC
GTAACCCCGAACGGGTAGAGGTGCTGCTGGCTGCATGCCGTAAGGAGAACGTCGAGGTCCGCGAGGCAGCCCTTGCGCTC
GCTGGGCTTACCGCGAAAGTGGTCGATGTGGAGGCGTATGCCCTCGAACGCGCTTACGGCCTGCTGGAGGCGCAGTTGGG
TGGTGGTCACGATGAGTTGACCGTCGCGGTGGTGGATATTGGCGCGACGATGACCACGCTCAGTGTGCTGCACAACGGTC
GTACCATCTACACCCGCGAGCAGCTTTTCGGCGGCAAGCAGCTGACTGAGGAAATCCAGCGTCGCTATGGCCTGTCGGTC
GAGGAAGCCGGTCTTGCCAAGAAGCAAGGTGGTCTTCCGGATGACTACGACAGTGAGGTTTTGCAACCGTTCAAGGAAGC
GGTCGTTCAGCAGGTTTCACGTTCGCTGCAATTCTTCTTCGCCGCCGGGCAATTCAATGATGTCGACTACATCCTCCTGG
CTGGGGGTACCGCGTCCATTCCTGATCTCGATCGGCTGATCCAGCAAAAAATCGGTACGCAGACGCTAGTGGCCAATCCC
TTTGCCGATATGGCGCTGAGCAGCAAGGTCAACGCCGGTGCGCTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A2S7FHW1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	44.915	100	0.449