Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   QUE89_RS03460 Genome accession   NZ_AP028070
Coordinates   761715..762779 (+) Length   354 a.a.
NCBI ID   WP_286221851.1    Uniprot ID   -
Organism   Marinobacter sp. LA51     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 756715..767779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE89_RS03445 rpmE 757254..757478 (+) 225 WP_041341598.1 50S ribosomal protein L31 -
  QUE89_RS03450 - 757701..758966 (+) 1266 WP_286221849.1 malic enzyme-like NAD(P)-binding protein -
  QUE89_RS03455 - 759052..761520 (-) 2469 WP_286221850.1 penicillin-binding protein 1A -
  QUE89_RS03460 pilM 761715..762779 (+) 1065 WP_286221851.1 pilus assembly protein PilM Machinery gene
  QUE89_RS03465 - 762779..763345 (+) 567 WP_286221852.1 PilN domain-containing protein -
  QUE89_RS03470 pilO 763342..763953 (+) 612 WP_286221853.1 type 4a pilus biogenesis protein PilO -
  QUE89_RS03475 - 763956..764504 (+) 549 WP_286221854.1 pilus assembly protein PilP -
  QUE89_RS03480 pilQ 764569..766632 (+) 2064 WP_286221855.1 type IV pilus secretin PilQ Machinery gene
  QUE89_RS03485 aroK 766771..767337 (+) 567 WP_286221856.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37915.02 Da        Isoelectric Point: 4.1452

>NTDB_id=103555 QUE89_RS03460 WP_286221851.1 761715..762779(+) (pilM) [Marinobacter sp. LA51]
MFGLFGKKSSAVLGVDVSSSSVKLLELSKQGGRIKVESYAVEPLPANAVVEKNITDVEAVGEVLKRVASKSRTGVKQVAV
AVSGSAVITKLIQMDGGLNEFEMEDQIALEADQYIPYPLDEVAIDFEVQGPSENNPDQVDVLLAACRKENVDIREDALEI
ASLSAKIVDVEAYSLERAYSLIEPQLDSQGEELVVAIVDVGATMTTLSVLADGKTVYTREQIFGGKQLTEEIQRRYGLSL
EEAGLAKKQGGLPDDYESEVLMPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIQGLTEMVEEKTGTPTLVANP
FADMAVGSRVNASALSNDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=103555 QUE89_RS03460 WP_286221851.1 761715..762779(+) (pilM) [Marinobacter sp. LA51]
GTGTTCGGATTGTTCGGAAAGAAATCCAGTGCAGTGCTGGGCGTGGACGTTAGCTCCAGTTCGGTCAAACTTCTGGAGCT
GTCAAAGCAGGGCGGCCGCATAAAAGTCGAAAGCTACGCGGTGGAGCCTTTGCCGGCTAACGCCGTTGTCGAGAAAAATA
TCACGGACGTCGAGGCAGTAGGTGAAGTGCTCAAGCGCGTCGCCTCGAAATCGCGCACGGGTGTGAAACAGGTTGCCGTG
GCCGTCTCAGGCTCTGCCGTGATCACCAAGCTCATCCAGATGGATGGCGGCCTCAACGAATTTGAGATGGAAGACCAGAT
TGCCCTTGAGGCTGATCAGTACATTCCATATCCGCTGGATGAAGTTGCCATCGACTTTGAGGTCCAGGGCCCTTCGGAAA
ACAATCCCGATCAGGTTGATGTTCTCCTGGCTGCATGCAGAAAGGAAAACGTCGACATACGTGAGGACGCGCTTGAGATT
GCCTCGCTGTCCGCAAAGATTGTTGATGTCGAAGCTTACTCCCTTGAGCGCGCTTATTCCTTGATCGAACCGCAGCTCGA
TTCCCAGGGCGAAGAACTGGTAGTGGCGATAGTCGATGTCGGTGCCACCATGACCACACTCAGCGTTCTCGCCGATGGCA
AGACCGTTTATACCCGCGAACAGATCTTCGGTGGCAAGCAGCTTACCGAAGAAATTCAGCGTCGTTACGGACTGTCGCTT
GAAGAAGCAGGCCTCGCCAAGAAGCAGGGCGGCTTGCCCGACGACTATGAGTCTGAAGTGTTGATGCCATTCCGCGAGGC
GGTGGTTCAGCAAGTAGCGCGGGCCTTGCAGTTCTTCTTTGGCGCTAGCCAATACAACGCAGTCGATTATGTGGTTCTGG
CAGGTGGTACTGCCTCGATACAGGGGCTGACAGAGATGGTCGAGGAAAAAACAGGAACGCCGACTTTGGTCGCCAACCCG
TTTGCCGACATGGCAGTAGGGTCCCGGGTGAATGCGTCTGCACTGAGTAACGATGCTCCCTCGCTGATGATTGCCTGTGG
CCTGGCAATGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.39

100

0.534
  comM Acinetobacter nosocomialis M2

53.39

100

0.534
  comM Acinetobacter baylyi ADP1

50.282

100

0.503
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.449

Multiple sequence alignment