Detailed information NTG1034856

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB9V60_RS13865
• Genome accession: NZ_CP166624
• Coordinates: 3206291..3207460 (-)
• Length: 389 a.a.
• NCBI ID: WP_003393947.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. atrofaciens strain GM 2231
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3201291..3212460

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB9V60_RS13850 (AB9V60_13850)	-	3202578..3203639 (+)	1062	WP_017279305.1	microcin C ABC transporter permease YejB	-
AB9V60_RS13855 (AB9V60_13855)	-	3203639..3204661 (+)	1023	WP_003318547.1	ABC transporter permease	-
AB9V60_RS13860 (AB9V60_13860)	-	3204663..3206240 (+)	1578	WP_044313051.1	ABC transporter ATP-binding protein	-
AB9V60_RS13865 (AB9V60_13865)	pilU	3206291..3207460 (-)	1170	WP_003393947.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB9V60_RS13870 (AB9V60_13870)	-	3207584..3207859 (+)	276	WP_002553334.1	peptidylprolyl isomerase	-
AB9V60_RS13875 (AB9V60_13875)	pgm	3207933..3209579 (-)	1647	WP_370683053.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
AB9V60_RS13880 (AB9V60_13880)	-	3209743..3212070 (-)	2328	WP_370683054.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42633.75 Da
• Isoelectric Point: 6.0641

>NTDB_id=1034856 AB9V60_RS13865 WP_003393947.1 3206291..3207460(-) (pilU) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQALMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHTEGGAGTLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=1034856 AB9V60_RS13865 WP_003393947.1 3206291..3207460(-) (pilU) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
ATGGATTTCCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTACCTCTCCACCGGAGCACCACCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGGGAGGTCGCAGTCATTGCCCAGGCGC
TTATGGACGAGGAGCAGAAGCTCGAGTTTCAGCGCGAACTGGAGATGAACCTCGCCGTTTCTATGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGACATCCCGCGTTTCGA
AGACCTGTTCCTGCCGCCCGTGCTGCTCGACGTGATCATGGAGAAGCGCGGTCTGGTGCTGTTTGTCGGGGCCACCGGTT
CCGGGAAATCGACCTCTTTGGCGGCGCTGATCGACTACCGTAACCGCAACGCCAGTGGCCATATCATCACCATTGAAGAC
CCGGTCGAGTTCATCCATCGCCACAAGAAGTCGATCGTCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGCTTCCATGC
GGCACTGAAGAACACCCTGCGCCAAGCGCCGGATGTGATTCTGATCGGCGAGATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACACTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAATTTCTTCCCGGAAGAGCGTCGCGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCATTCGTCTCTCAGCG
ACTGGTCAGAACCCCGGATGGCAAGCGTCGTGCGGCGGTGGAAGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAGTCCGGCAGCCTCGGTATGCAGACGTTCGACACGGCGCTA
TTCAATCTGGCCGTCGAAGGTGCGATCAGCGAAGAAGAGGCCCTCAAGAACGCTGACTCGCAAAACAACGTGCGCCTGCG
CCTCAAACTGCACACTGAAGGCGGCGCCGGGACCCTGACCACACCGCCGCCGGCCCCGACCGGCAGCAGCACGGCAAGCA
CCGCCGAGTGGGGGCTGGTCGATGATGACGCGCCGGGTCCGCAGGCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.735	93.059	0.537
pilU	Vibrio cholerae strain A1552	51.105	93.059	0.476
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.903	98.458	0.383
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362