Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AB8806_RS02220 Genome accession   NZ_CP166102
Coordinates   450550..452277 (-) Length   575 a.a.
NCBI ID   WP_369952027.1    Uniprot ID   -
Organism   Ralstonia syzygii subsp. celebesensis strain NCPPB 3728     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 445550..457277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8806_RS02190 (AB8806_02190) - 446170..446628 (+) 459 WP_048817320.1 NUDIX domain-containing protein -
  AB8806_RS02195 (AB8806_02195) - 446625..446840 (-) 216 WP_013211401.1 DNA gyrase inhibitor YacG -
  AB8806_RS02200 (AB8806_02200) zapD 446852..447610 (-) 759 WP_013211402.1 cell division protein ZapD -
  AB8806_RS02205 (AB8806_02205) coaE 447728..448342 (-) 615 WP_078222780.1 dephospho-CoA kinase -
  AB8806_RS02210 (AB8806_02210) pilD 448355..449236 (-) 882 WP_078222781.1 A24 family peptidase Machinery gene
  AB8806_RS02215 (AB8806_02215) - 449239..450504 (-) 1266 WP_078222782.1 type II secretion system F family protein -
  AB8806_RS02220 (AB8806_02220) pilF 450550..452277 (-) 1728 WP_369952027.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB8806_RS02225 (AB8806_02225) - 452525..453802 (-) 1278 WP_013211407.1 HlyC/CorC family transporter -
  AB8806_RS02235 (AB8806_02235) ispB 454081..455049 (-) 969 WP_078222784.1 octaprenyl diphosphate synthase -
  AB8806_RS02240 (AB8806_02240) rplU 455328..455639 (+) 312 WP_003271629.1 50S ribosomal protein L21 -
  AB8806_RS02245 (AB8806_02245) rpmA 455677..455937 (+) 261 WP_004633021.1 50S ribosomal protein L27 -
  AB8806_RS02250 (AB8806_02250) obgE 456125..457225 (+) 1101 WP_013211409.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63623.35 Da        Isoelectric Point: 7.1994

>NTDB_id=1033434 AB8806_RS02220 WP_369952027.1 450550..452277(-) (pilF) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3728]
MTLGLALAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDAVPAVLSTNKHF
QQLRLLPLGRRDNRIIVATSDPSDPKPIEELRQKMNVAIETVIVEHDKLVRQLSLANEAPTEIKSLIPKQEATQIEYDPG
AAAAVRRTTTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILAQVAQPPLDIRDKIATRIKVLSRL
DISEKHVPQDGRMKLLITVPKDKNDKDNKETIERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLTNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPRETL
LEAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILSHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1033434 AB8806_RS02220 WP_369952027.1 450550..452277(-) (pilF) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3728]
ATGACTCTCGGACTCGCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCACCCAGCTTGAACAAAGCGCACGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCGCACGATCTCGCCGTCTTCGTGGCGGGCA
AGTACCAGATGCCGCTGCTGGACCTGGCGCAGTACAAGCTCGATGCCGTGCCGGCCGTGCTGTCGACCAACAAGCATTTC
CAGCAATTGCGCCTGTTGCCGCTGGGCCGGCGCGACAACCGCATCATCGTTGCCACCTCGGACCCGAGCGACCCCAAGCC
GATCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCGCCAACGAAGCGCCGACCGAGATCAAGTCGCTCATCCCCAAGCAGGAAGCCACGCAGATCGAATACGACCCGGGC
GCAGCCGCTGCAGTGCGGCGTACCACGACCGACGGCATCGACGATGCGCCGGTGGTGCGTTTCCTGCAAAAGCTGTTTAC
CGAAGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGTATCGACG
GCATCCTCGCGCAGGTCGCGCAGCCACCGCTGGATATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATCTCGGAAAAGCACGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACGGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGACGATCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCCACCGAGCGGCTCGATATCGACCAGCTAGGCTATGAGCCCGAGCAAAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAAACGGTCTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAAGCGGGGCTGACCTTCGCCGCGGCGCTGAAATCGTTCCTGCGGCAGGATCCGGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGATGCGCCGACCACGCTCACGCGGCTGACGAACATGGGCGTGGCACCGTTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCATGCAAGAAGCCGGGCCCGCTGCCCCGGGAAACACTG
CTGGAGGCCGGCTTCAAGGAAGCCGACCTGGACGGCTCGTGGCAGCCGTACCATCCAGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCAGCCACG
GCACGGCACTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCTGGCCTGCGGAAAGTGAAA
CAGGGGCTGACTTCCCTTGAGGAAGTGCTGGCCACGACGAACCAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)

Type II/IV secretion system protein (T2SSE)

(184-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.333

93.913

0.501
  pilB Acinetobacter baumannii D1279779

50.973

98.261

0.501
  pilB Acinetobacter baylyi ADP1

49.911

97.913

0.489
  pilB Legionella pneumophila strain ERS1305867

46.41

99.304

0.461
  pilB Vibrio cholerae strain A1552

44.604

96.696

0.431
  pilB Vibrio campbellii strain DS40M4

44.649

94.261

0.421
  pilB Vibrio parahaemolyticus RIMD 2210633

44.465

94.261

0.419
  pilF Thermus thermophilus HB27

37.522

98.261

0.369

Multiple sequence alignment