Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB8814_RS06180 Genome accession   NZ_CP166090
Coordinates   1479958..1481694 (+) Length   578 a.a.
NCBI ID   WP_205393987.1    Uniprot ID   -
Organism   Xanthomonas hydrangeae strain NCPPB 4692     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1474958..1486694
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8814_RS06160 (AB8814_06160) - 1475968..1476804 (+) 837 WP_205393984.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  AB8814_RS06165 (AB8814_06165) - 1476801..1477718 (+) 918 WP_205393985.1 lysylphosphatidylglycerol synthase domain-containing protein -
  AB8814_RS06170 (AB8814_06170) - 1477710..1478876 (-) 1167 WP_205393986.1 glycosyltransferase -
  AB8814_RS06175 (AB8814_06175) - 1478869..1479726 (-) 858 WP_104592954.1 glycosyltransferase family 2 protein -
  AB8814_RS06180 (AB8814_06180) pilB 1479958..1481694 (+) 1737 WP_205393987.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB8814_RS06185 (AB8814_06185) pilR 1481824..1483218 (-) 1395 WP_232372276.1 sigma-54 dependent transcriptional regulator Regulator
  AB8814_RS06190 (AB8814_06190) - 1483364..1484977 (-) 1614 WP_205393989.1 PAS domain-containing sensor histidine kinase -
  AB8814_RS06195 (AB8814_06195) sucC 1485210..1486379 (+) 1170 WP_205393990.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62667.81 Da        Isoelectric Point: 5.6078

>NTDB_id=1033315 AB8814_RS06180 WP_205393987.1 1479958..1481694(+) (pilB) [Xanthomonas hydrangeae strain NCPPB 4692]
MTAALTANLVGITGIARRLVQDGALDEAAARTAMDQAGAARVPLPQWFAEKKLVTASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLG
DDDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTAEQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTEEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1033315 AB8814_RS06180 WP_205393987.1 1479958..1481694(+) (pilB) [Xanthomonas hydrangeae strain NCPPB 4692]
ATGACCGCTGCGCTAACTGCAAACTTGGTTGGTATTACGGGTATCGCTCGTCGATTGGTTCAGGACGGTGCGCTGGATGA
AGCTGCTGCACGTACTGCCATGGACCAAGCCGGGGCGGCAAGAGTCCCGCTGCCACAATGGTTTGCAGAGAAAAAGCTGG
TCACTGCATCCCAGCTTGCCGCTGCAAATGCAGTCGAGTTCGGCATGCCCCTGATGGACGTTTCCGTGTTCGATGCTAGC
CAGAACGCGGTCAAGCTGGTCAGTGAGGAGTTGCTCCAGAAGTACCAAGTGCTGCCGCTTTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTTCATACCAACCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGTCGCACCTTGGAGCAGTGGCAGGCCAGCAACGCGTCGTTGGGCTCGTCGCTCGGG
GACGATGACGAGGGGATGGGCGACCTGGACGTGTCGGCCGGTGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCATTTTGAGCCCTATGAAGACGACTACCGGGTGCGGCTGCGGATCGATGGGCTGCTGAAGAATGTGGCCAAGGCACCG
GTAAAGCTGAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATTGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTTGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAACCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACTGTGTCGCT
GTATACGGCGCTGGGCATCCTCAACGACGAGACGCGCAATATCTCCACCGCCGAGGACCCGGTAGAAATCCGCCTGCCCG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTACGCTCGTTTTTGCGCCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCCATCAAGGCCGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCACACCAACGACGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGCATCGCGCCGTACAACATCA
CCTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCC
GAGCATGCGTTGCTTGCGGAAGGGTTCACTGCAGAGCAGCTCGCTGCCGGTATCGAGCTATACGAAGCGGTCGGCTGCGA
CGAGTGCACCGAAGGCTATAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGAAGAGATCGGCGCGATCGTGC
TGGAAGGCGGCAACGCGATGCAGATCGCCGAGGCCGCGCAGAAGATCGGCATCCGCGATCTGCGGCAGTCGGCGCTGGTG
AAGGCGGCGCATGGGGTGACGAGCCTGGCTGAGATCAATCGGGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

98.27

0.552
  pilB Acinetobacter baylyi ADP1

55.282

98.27

0.543
  pilB Legionella pneumophila strain ERS1305867

52.021

98.443

0.512
  pilF Neisseria gonorrhoeae MS11

49.296

98.27

0.484
  pilB Vibrio cholerae strain A1552

48.611

99.654

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

46.643

97.924

0.457
  pilB Vibrio campbellii strain DS40M4

45.694

98.443

0.45
  pilB/pilB1 Synechocystis sp. PCC 6803

36.098

100

0.384

Multiple sequence alignment