NTDB

Overview

Name	ruvB	Type	Machinery gene
Locus tag	AW03_RS13395	Genome accession	NZ_CP007173
Coordinates	2597146..2598150 (-)	Length	334 a.a.
NCBI ID	WP_003229718.1	Uniprot ID	O32055
Organism	Bacillus subtilis HJ5
Function	homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2592146..2603150

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AW03_RS13365 (AW03_025810)	yrbG	2593120..2593776 (-)	657	WP_015483509.1	DUF421 domain-containing protein	-
AW03_RS13370 (AW03_025820)	-	2593974..2594363 (+)	390	WP_015483510.1	TIGR04086 family membrane protein	-
AW03_RS13375 (AW03_025830)	yajC	2594420..2594686 (-)	267	WP_004398708.1	preprotein translocase subunit YajC	-
AW03_RS13380 (AW03_025840)	tgt	2594723..2595868 (-)	1146	WP_003229725.1	tRNA guanosine(34) transglycosylase Tgt	-
AW03_RS13385 (AW03_025850)	queA	2595895..2596923 (-)	1029	WP_015483511.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
AW03_RS13390 (AW03_025860)	yrzS	2596953..2597153 (-)	201	WP_015384252.1	DUF2905 domain-containing protein	-
AW03_RS13395 (AW03_025870)	ruvB	2597146..2598150 (-)	1005	WP_003229718.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
AW03_RS13400 (AW03_025880)	ruvA	2598161..2598766 (-)	606	WP_003229717.1	Holliday junction branch migration protein RuvA	Machinery gene
AW03_RS13405 (AW03_025890)	bofC	2598905..2599417 (-)	513	WP_003229715.1	sporulation cell-cell signaling protein BofC	-
AW03_RS13410 (AW03_025900)	csbX	2599465..2600772 (-)	1308	WP_015483512.1	MFS transporter	-
AW03_RS13415 (AW03_025910)	iolG	2600843..2601868 (-)	1026	WP_015483513.1	inositol 2-dehydrogenase	-
AW03_RS13420 (AW03_025920)	yrzF	2602106..2602753 (+)	648	WP_015483514.1	phosphotransferase	-
AW03_RS21745 (AW03_025930)	-	2602799..2602921 (-)	123	WP_003229707.1	hypothetical protein	-

Sequence

Protein

Download Length: 334 a.a. Molecular weight: 37467.78 Da Isoelectric Point: 5.1631

                                >NTDB_id=102779 AW03_RS13395 WP_003229718.1 2597146..2598150(-) (ruvB) [Bacillus subtilis HJ5]

MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE

LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT

RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI

TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP

AVYHHFQMEAPRYD

Nucleotide

Download Length: 1005 bp

                                >NTDB_id=102779 AW03_RS13395 WP_003229718.1 2597146..2598150(-) (ruvB) [Bacillus subtilis HJ5]

ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC

ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG

ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCCATTGTAGCCAATGAAATGGGAGTGGAA

TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT

ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG

ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGGCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACG

AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTGATGTCTCGCCTTGAGTACTATACACAAGAAGAACT

GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT

CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT

ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT

GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA

CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG

GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	100	100	1
ruvB	Streptococcus pneumoniae TIGR4	60.429	97.605	0.59
ruvB	Streptococcus pneumoniae R6	60.429	97.605	0.59
ruvB	Streptococcus pneumoniae D39	60.429	97.605	0.59
ruvB	Synechocystis sp. PCC 6803	57.143	98.503	0.563
ruvB	Helicobacter pylori 26695	52.744	98.204	0.518

Detailed information NTG0102779