Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PA5486_RS28425 Genome accession   NZ_LS998783
Coordinates   5804816..5806075 (+) Length   419 a.a.
NCBI ID   WP_134163279.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa isolate     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5799816..5811075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PA5486_RS28405 (PA5486_05522) - 5799885..5801198 (-) 1314 WP_015648064.1 O-antigen ligase family protein -
  PA5486_RS28410 (PA5486_05523) pilA/pilAI 5801356..5801820 (-) 465 WP_023129290.1 pilin Machinery gene
  PA5486_RS28415 (PA5486_05524) pilB 5802051..5803751 (+) 1701 WP_030048379.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PA5486_RS28420 (PA5486_05525) - 5803822..5804838 (+) 1017 WP_032728035.1 IS30-like element IS1394 family transposase -
  PA5486_RS28425 (PA5486_05526) pilC 5804816..5806075 (+) 1260 WP_134163279.1 type II secretion system F family protein Machinery gene
  PA5486_RS28430 (PA5486_05527) pilD 5806076..5806948 (+) 873 WP_015648060.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PA5486_RS28435 (PA5486_05528) coaE 5806945..5807556 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PA5486_RS28440 (PA5486_05529) yacG 5807553..5807753 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PA5486_RS28445 (PA5486_05530) - 5807790..5807999 (-) 210 WP_003094660.1 hypothetical protein -
  PA5486_RS28450 (PA5486_05531) - 5808105..5808794 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  PA5486_RS28455 (PA5486_05532) - 5808791..5809261 (-) 471 WP_003103866.1 hypothetical protein -
  PA5486_RS28460 (PA5486_05533) - 5809258..5809683 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  PA5486_RS28465 (PA5486_05534) - 5809816..5810445 (+) 630 WP_015648058.1 DUF1780 domain-containing protein -
  PA5486_RS28470 (PA5486_05535) - 5810442..5810891 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 45665.79 Da        Isoelectric Point: 9.9077

>NTDB_id=1027148 PA5486_RS28425 WP_134163279.1 5804816..5806075(+) (pilC) [Pseudomonas aeruginosa isolate]
MHLLQSSNRVALSFCNRQALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPM
DIALFTRQMSTMMAAGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGAL
ETLLDRVATYKEKTEALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTMMVINISNVLQEWWL
LVLLMMGGAGFLLNHAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFR
DAVGKIKQDVSTGMQLNFSMRTTHIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVL
VGGLIIAMYLPIFQLGNVV

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=1027148 PA5486_RS28425 WP_134163279.1 5804816..5806075(+) (pilC) [Pseudomonas aeruginosa isolate]
ATGCACCTGCTTCAATCCAGTAACCGTGTTGCACTCAGCTTCTGCAACCGCCAAGCGTTAAAGACCAGTGTCTTCGTATG
GGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCTGTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGC
GCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCATCTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATG
GACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCCGCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCATCAG
CGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGAGATCAAGCAGGAAGTCGCCGGCGGCAACAGCCTGGCCA
ACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACTGCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTG
GAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACCGAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTA
CCCGATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGATCCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGT
TCGAGGGCTTCGGCGCCGAACTGCCAGCCTTCACCATGATGGTCATCAACATTTCCAACGTACTCCAGGAATGGTGGCTC
CTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAACCACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCAC
CGACCGCACGGTACTGAAGCTGCCTATCGTCGGCGCCATCCTCTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGT
CGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCGACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGG
GACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATGCAGCTGAACTTCTCCATGCGCACCACCCACATCTTCCC
CAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAGTCGGGCGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCT
ACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCCTGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTG
GTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAGCTGGGCAACGTCGTCTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(85-208)

Type II secretion system (T2SS), protein F (T2SSF)

(290-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.412

96.181

0.754
  pilC Acinetobacter baylyi ADP1

61.5

95.465

0.587
  pilC Acinetobacter baumannii D1279779

59.753

96.659

0.578
  pilC Legionella pneumophila strain ERS1305867

54.545

94.511

0.516
  pilG Neisseria gonorrhoeae MS11

46.04

96.42

0.444
  pilG Neisseria meningitidis 44/76-A

45.522

95.943

0.437
  pilC Vibrio cholerae strain A1552

41.687

96.181

0.401
  pilC Vibrio campbellii strain DS40M4

40.852

95.227

0.389
  pilC Thermus thermophilus HB27

38.155

95.704

0.365

Multiple sequence alignment