Detailed information NTG1026881

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB3471_RS12820
• Genome accession: NZ_CP162520
• Coordinates: 2937058..2938227 (+)
• Length: 389 a.a.
• NCBI ID: WP_003318544.1
• Uniprot ID: A0A0P9GM94
• Organism: Pseudomonas syringae pv. atrofaciens strain UPB 463
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2932058..2943227

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB3471_RS12805 (AB3471_12775)	-	2932447..2934774 (+)	2328	WP_004417522.1	ATP-dependent DNA helicase	-
AB3471_RS12810 (AB3471_12780)	pgm	2934938..2936584 (+)	1647	WP_004417524.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
AB3471_RS12815 (AB3471_12785)	-	2936659..2936934 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
AB3471_RS12820 (AB3471_12790)	pilU	2937058..2938227 (+)	1170	WP_003318544.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB3471_RS12825 (AB3471_12795)	-	2938278..2939855 (-)	1578	WP_004417526.1	ABC transporter ATP-binding protein	-
AB3471_RS12830 (AB3471_12800)	-	2939857..2940879 (-)	1023	WP_003318547.1	ABC transporter permease	-
AB3471_RS12835 (AB3471_12805)	-	2940879..2941940 (-)	1062	WP_003318548.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42619.73 Da
• Isoelectric Point: 6.0641

>NTDB_id=1026881 AB3471_RS12820 WP_003318544.1 2937058..2938227(+) (pilU) [Pseudomonas syringae pv. atrofaciens strain UPB 463]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQALMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGTLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=1026881 AB3471_RS12820 WP_003318544.1 2937058..2938227(+) (pilU) [Pseudomonas syringae pv. atrofaciens strain UPB 463]
ATGGATTTCCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTACCTCTCCACCGGAGCACCACCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGGGAGGTCGCAGTCATTGCCCAGGCGC
TTATGGACGAGGAGCAGAAGCTCGAGTTTCAGCGCGAACTGGAGATGAACCTCGCCGTTTCTATGGCGGGTATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGACATCCCGCGTTTCGA
AGACCTGTTCCTGCCGCCCGTGCTGCTCGACGTGATCATGGAGAAGCGCGGTCTGGTGTTGTTTGTCGGGGCCACCGGTT
CCGGGAAATCGACCTCTTTGGCGGCGCTGATCGACTACCGTAACCGCAACGCCAGTGGCCATATCATCACCATTGAAGAC
CCGGTCGAGTTCATCCATCGCCACAAGAAGTCGATCGTCAACCAGCGCGAAGTCGGGGTCGATACTCGCAGCTTCCATGC
GGCACTGAAGAACACCCTGCGCCAAGCGCCGGATGTGATTCTGATCGGCGAGATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACACTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAATTTCTTCCCGGAAGAGCGTCGCGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCATTCGTCTCTCAGCG
ACTGGTCAGAACCCCGGATGGCAAGCGTCGTGCGGCGGTGGAAGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAGTCCGGCAGCCTCGGTATGCAGACGTTCGACACGGCGCTA
TTCAATCTGGCCGTCGAAGGTGCGATCAGCGAAGAAGAGGCCCTCAAGAACGCTGACTCGCAAAACAACGTGCGCCTGCG
CCTCAAACTGCACAGTGAAGGCGGCGCCGGGACCCTGACCACACCGCCGCCGGCCCCGACCGGCAGCAGCACGGCAAGCA
CCGCCGAGTGGGGGCTGGTCGATGATGATGCGCCGGGTCCGCAGGCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-270)

3D structure

• AlphaFold DB: A0A0P9GM94

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.011	93.059	0.54
pilU	Vibrio cholerae strain A1552	51.105	93.059	0.476
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.903	98.458	0.383
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362