Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   AB3443_RS02625 Genome accession   NZ_CP162437
Coordinates   504496..505566 (+) Length   356 a.a.
NCBI ID   WP_000775708.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM22698     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 499496..510566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3443_RS02595 (AB3443_02595) - 499919..501382 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  AB3443_RS02600 (AB3443_02600) - 501363..501566 (+) 204 WP_000087561.1 YqgQ family protein -
  AB3443_RS02605 (AB3443_02605) - 501563..502549 (+) 987 WP_000161314.1 ROK family glucokinase -
  AB3443_RS02610 (AB3443_02610) - 502549..502878 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  AB3443_RS02615 (AB3443_02615) - 502875..503498 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  AB3443_RS02620 (AB3443_02620) comGA 503550..504524 (+) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  AB3443_RS02625 (AB3443_02625) comGB 504496..505566 (+) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  AB3443_RS02630 (AB3443_02630) comGC 505580..505891 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  AB3443_RS02635 (AB3443_02635) comGD 505869..506315 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  AB3443_RS02640 (AB3443_02640) comGE 506302..506601 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  AB3443_RS02645 (AB3443_02645) comGF 506519..506941 (+) 423 WP_258922346.1 competence type IV pilus minor pilin ComGF Machinery gene
  AB3443_RS02650 (AB3443_02650) - 507019..508191 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  AB3443_RS02655 (AB3443_02655) - 508244..508348 (+) 105 Protein_487 type IV pilus minor pilin ComGF family protein -
  AB3443_RS02660 (AB3443_02660) - 508445..508591 (+) 147 WP_001789879.1 hypothetical protein -
  AB3443_RS02665 (AB3443_02665) - 508581..509105 (+) 525 WP_001015117.1 shikimate kinase -
  AB3443_RS02670 (AB3443_02670) gcvT 509264..510355 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41364.77 Da        Isoelectric Point: 10.0092

>NTDB_id=1025505 AB3443_RS02625 WP_000775708.1 504496..505566(+) (comGB) [Staphylococcus aureus strain TUM22698]
MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1025505 AB3443_RS02625 WP_000775708.1 504496..505566(+) (comGB) [Staphylococcus aureus strain TUM22698]
GTGAAACTACAATGCATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGACTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.719

100

0.997
  comGB Staphylococcus aureus N315

99.719

100

0.997

Multiple sequence alignment