Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   AB3G27_RS16150 Genome accession   NZ_CP162095
Coordinates   3157609..3158736 (-) Length   375 a.a.
NCBI ID   WP_368197169.1    Uniprot ID   -
Organism   Bacillus pumilus strain LBUM494     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3152609..3163736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3G27_RS16110 (AB3G27_16110) flgL 3152789..3153700 (-) 912 WP_137054345.1 flagellar hook-associated protein FlgL -
  AB3G27_RS16115 (AB3G27_16115) flgK 3153710..3155230 (-) 1521 WP_003213544.1 flagellar hook-associated protein FlgK -
  AB3G27_RS16120 (AB3G27_16120) - 3155246..3155728 (-) 483 WP_003213076.1 flagellar protein FlgN -
  AB3G27_RS16125 (AB3G27_16125) flgM 3155747..3156010 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  AB3G27_RS16130 (AB3G27_16130) - 3156090..3156509 (-) 420 WP_003212865.1 TIGR03826 family flagellar region protein -
  AB3G27_RS16135 (AB3G27_16135) comFC 3156566..3157072 (-) 507 WP_050782676.1 ComF family protein Machinery gene
  AB3G27_RS16140 (AB3G27_16140) - 3157085..3157252 (-) 168 Protein_3121 ComF family protein -
  AB3G27_RS16145 (AB3G27_16145) - 3157249..3157542 (-) 294 WP_106066158.1 late competence development ComFB family protein -
  AB3G27_RS16150 (AB3G27_16150) comFA 3157609..3158736 (-) 1128 WP_368197169.1 DEAD/DEAH box helicase Machinery gene
  AB3G27_RS16155 (AB3G27_16155) - 3159108..3159950 (-) 843 WP_003213402.1 DegV family protein -
  AB3G27_RS16160 (AB3G27_16160) degU 3160168..3160857 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  AB3G27_RS16165 (AB3G27_16165) degS 3160922..3162097 (-) 1176 WP_003213166.1 sensor histidine kinase Regulator
  AB3G27_RS16170 (AB3G27_16170) - 3162319..3162987 (+) 669 WP_368197173.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42885.30 Da        Isoelectric Point: 10.4905

>NTDB_id=1024011 AB3G27_RS16150 WP_368197169.1 3157609..3158736(-) (comFA) [Bacillus pumilus strain LBUM494]
MMGKTTECGFLYEWTGPQMEEACHSELTWQGNLSKGQKRASEKLIEAIKNKSDLLIWAVCGAGKTEVLFHGIEYALHQGM
SVCIATPRTDVVLELEPRLKKAFQGMKIAVLYGGSPQRFQIEKLVIATTHQLMRYKHAFDVLIIDEVDAFPYSIDGRLQF
AVLKAMRKKGVRVYLSATPSKKMIRDVSRGQLEAIKIPLRFHQQPLPVPSFQWIGHWKKKLKKNQLPPKVMNWMQKHITK
KRRVLLFVPSISTMKKVTKVLRKHHLNVEGVSADDPDRKQKVQQFRDYKYDVLVTTTILERGVTISNVQVGVLGAESTIF
TESALVQISGRAGRHPDFFKGDVFFFHFGLTRSMKQAMKHIVKMNDTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1024011 AB3G27_RS16150 WP_368197169.1 3157609..3158736(-) (comFA) [Bacillus pumilus strain LBUM494]
ATGATGGGGAAAACAACGGAATGTGGCTTTCTTTATGAATGGACGGGTCCACAAATGGAAGAAGCATGTCACTCGGAATT
AACATGGCAGGGAAACCTGTCGAAAGGACAAAAAAGAGCGTCAGAGAAATTGATCGAAGCGATAAAAAACAAATCTGATC
TACTCATCTGGGCAGTTTGCGGGGCAGGGAAAACAGAGGTGCTTTTTCACGGAATAGAATATGCCTTACATCAAGGAATG
AGTGTCTGCATTGCGACACCTAGAACTGATGTTGTCCTTGAACTAGAGCCGCGGCTTAAAAAAGCTTTTCAAGGAATGAA
AATAGCTGTTCTTTATGGAGGAAGTCCTCAAAGGTTCCAAATAGAAAAGTTAGTAATCGCTACAACTCACCAGTTGATGA
GATATAAACATGCATTTGATGTGCTCATTATAGATGAAGTCGACGCCTTCCCTTATTCAATCGATGGACGTCTCCAATTT
GCTGTTTTAAAGGCAATGAGAAAGAAAGGGGTTAGGGTTTATTTAAGTGCGACGCCGTCTAAAAAAATGATAAGAGATGT
ATCTCGTGGACAACTAGAAGCCATAAAAATTCCTCTGCGCTTTCACCAACAACCTTTACCGGTCCCATCCTTTCAATGGA
TTGGACATTGGAAGAAGAAATTAAAAAAGAATCAGCTCCCACCTAAAGTAATGAATTGGATGCAGAAACATATTACAAAA
AAGAGAAGGGTTTTACTTTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACGAAGGTTCTTAGAAAGCACCACTTAAA
TGTAGAAGGGGTGTCTGCCGATGATCCAGATAGGAAACAAAAGGTCCAGCAATTCAGAGATTATAAATACGATGTACTAG
TTACAACCACAATTCTAGAAAGAGGTGTAACCATTTCAAATGTACAAGTGGGAGTACTAGGTGCAGAATCTACTATCTTT
ACAGAAAGTGCACTTGTTCAGATTTCTGGTAGAGCAGGTAGACATCCCGATTTTTTTAAAGGAGATGTTTTCTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAATGAAGCATATTGTAAAAATGAATGATACAGCTGAAAAAGAGTTCTCCG
AAAAATAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(236-335)

Type III restriction enzyme, res subunit (ResIII)

(32-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

58.108

98.667

0.573
  comFA Latilactobacillus sakei subsp. sakei 23K

39.247

99.2

0.389
  comFA/cflA Streptococcus mitis SK321

40.395

94.4

0.381
  comFA/cflA Streptococcus pneumoniae Rx1

39.831

94.4

0.376
  comFA/cflA Streptococcus pneumoniae D39

39.831

94.4

0.376
  comFA/cflA Streptococcus pneumoniae R6

39.831

94.4

0.376
  comFA/cflA Streptococcus pneumoniae TIGR4

39.548

94.4

0.373
  comFA/cflA Streptococcus mitis NCTC 12261

39.266

94.4

0.371
  comFA Lactococcus lactis subsp. cremoris KW2

37.883

95.733

0.363

Multiple sequence alignment