Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB2N10_RS11760 Genome accession   NZ_CP160906
Coordinates   2570991..2572106 (-) Length   371 a.a.
NCBI ID   WP_354625395.1    Uniprot ID   -
Organism   Psychromonas sp. MME1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2565991..2577106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB2N10_RS11730 - 2566355..2567116 (+) 762 WP_354625400.1 hypothetical protein -
  AB2N10_RS11735 mobB 2567169..2567693 (-) 525 WP_369433878.1 molybdopterin-guanine dinucleotide biosynthesis protein B -
  AB2N10_RS11740 - 2567821..2568453 (+) 633 WP_354625398.1 LysE family translocator -
  AB2N10_RS11745 - 2568508..2569422 (-) 915 WP_369433879.1 aldo/keto reductase family oxidoreductase -
  AB2N10_RS11750 - 2569870..2570055 (+) 186 WP_354625397.1 hypothetical protein -
  AB2N10_RS11755 yaaA 2570143..2570916 (-) 774 WP_354625396.1 peroxide stress protein YaaA -
  AB2N10_RS11760 pilU 2570991..2572106 (-) 1116 WP_354625395.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB2N10_RS11765 pilT 2572120..2573154 (-) 1035 WP_354625394.1 type IV pilus twitching motility protein PilT Machinery gene
  AB2N10_RS11770 - 2573176..2573874 (+) 699 WP_354625490.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB2N10_RS11775 proC 2573901..2574725 (+) 825 WP_354625393.1 pyrroline-5-carboxylate reductase -
  AB2N10_RS11780 - 2574750..2575292 (+) 543 WP_354625392.1 YggT family protein -
  AB2N10_RS11785 yggU 2575292..2575594 (+) 303 WP_369433880.1 DUF167 family protein YggU -
  AB2N10_RS11790 - 2575588..2576028 (+) 441 WP_354625390.1 DUF4426 domain-containing protein -
  AB2N10_RS11795 rdgB 2576042..2576643 (+) 602 Protein_2284 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41367.15 Da        Isoelectric Point: 6.4414

>NTDB_id=1022236 AB2N10_RS11760 WP_354625395.1 2570991..2572106(-) (pilU) [Psychromonas sp. MME1]
MLTALLHKASITKASDLFISVGLPATMKVNGYLEQINDQSLTSAEVYQLCEEAMGAQRFAKFKIEKESNFALNTVESGRF
RVSAYMQKEQPSMVIRRIEGNIPSFEELHLPQQLKETCMANHGLILCVGATGSGKSTTQAAMIGYRNENAAGHILTIEDP
IEFVHQHGRSVITQREVGIDTDSFENALKNSLRQAPDVILVGEIRNQETMEFTLNFAETGHLCLATLHANNANQALDRIM
HLVDKDRHRQFLFDLSVNLRAIVAQQLVPTADGKGRRVAFEILYNTPTMADAIRKGEFHELKNIMKKSTEQGMKTFDQSL
FELYERGIIGYTETLAHADSANDVRLMIKLHSTTDKKSFSAGALDDVTLGF

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=1022236 AB2N10_RS11760 WP_354625395.1 2570991..2572106(-) (pilU) [Psychromonas sp. MME1]
ATGTTAACGGCATTACTGCATAAAGCATCGATAACAAAAGCGTCTGATCTTTTTATCTCCGTAGGGTTACCTGCAACGAT
GAAAGTGAATGGTTATTTAGAGCAAATCAATGATCAAAGCTTAACCTCTGCGGAGGTTTATCAACTCTGTGAAGAGGCGA
TGGGGGCACAACGATTCGCTAAATTCAAGATTGAAAAAGAGTCTAATTTTGCTTTAAATACGGTTGAATCTGGGCGTTTT
CGTGTTAGTGCCTATATGCAAAAAGAGCAACCTTCAATGGTTATTCGTCGCATTGAAGGTAATATCCCAAGTTTTGAAGA
GTTACACCTGCCGCAGCAATTAAAAGAAACTTGTATGGCGAATCATGGCTTAATTCTCTGTGTGGGTGCAACGGGCTCTG
GTAAGTCAACAACGCAAGCTGCGATGATAGGATACCGTAATGAAAATGCCGCAGGGCATATTTTAACCATTGAAGATCCC
ATTGAATTTGTGCATCAACATGGGCGTTCGGTGATCACTCAACGAGAAGTGGGTATTGATACCGATAGCTTTGAAAACGC
ATTGAAAAACTCATTACGACAAGCACCTGATGTTATTTTGGTGGGGGAGATTCGTAATCAGGAAACCATGGAGTTTACCC
TTAATTTTGCGGAAACAGGACATCTCTGTTTAGCAACATTACATGCCAATAATGCTAACCAAGCACTTGATCGCATTATG
CATTTGGTTGATAAGGATCGTCATCGTCAATTTTTATTTGATTTATCGGTAAATTTACGTGCGATAGTTGCACAGCAGTT
AGTGCCTACTGCCGATGGCAAAGGACGGCGTGTCGCATTTGAAATTTTATATAATACCCCGACCATGGCTGATGCTATTC
GTAAGGGAGAGTTCCATGAGCTTAAAAATATTATGAAAAAATCGACGGAACAAGGTATGAAAACCTTTGATCAATCTCTA
TTTGAGTTATATGAGAGAGGCATTATTGGTTATACGGAAACCTTGGCGCATGCCGATTCGGCTAATGATGTTCGCTTAAT
GATCAAACTACACTCAACGACCGATAAAAAAAGTTTTAGCGCAGGCGCGTTAGATGATGTGACATTAGGGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.908

93.801

0.553
  pilU Vibrio cholerae strain A1552

56.863

96.226

0.547
  pilU Acinetobacter baylyi ADP1

54.108

95.148

0.515
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.356

90.836

0.367

Multiple sequence alignment