Detailed information NTG1022225

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AB2N10_RS00975
• Genome accession: NZ_CP160906
• Coordinates: 219517..220677 (+)
• Length: 386 a.a.
• NCBI ID: WP_354624697.1
• Uniprot ID: -
• Organism: Psychromonas sp. MME1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 214517..225677

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB2N10_RS00955	-	214522..215202 (-)	681	WP_354624693.1	exonuclease domain-containing protein	-
AB2N10_RS00960	-	215202..217046 (-)	1845	WP_354624694.1	DUF294 nucleotidyltransferase-like domain-containing protein	-
AB2N10_RS00965	-	217102..218820 (-)	1719	WP_354624695.1	sodium:solute symporter family protein	-
AB2N10_RS00970	-	218832..219098 (-)	267	WP_354624696.1	DUF4212 domain-containing protein	-
AB2N10_RS00975	pilU	219517..220677 (+)	1161	WP_354624697.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB2N10_RS00980	-	221135..221422 (+)	288	WP_354624698.1	hypothetical protein	-
AB2N10_RS00985	-	221794..222789 (+)	996	WP_354624505.1	hypothetical protein	-
AB2N10_RS00990	-	223275..223907 (+)	633	WP_369434184.1	reverse transcriptase domain-containing protein	-
AB2N10_RS00995	-	223855..224871 (+)	1017	WP_369434185.1	TIR domain-containing protein	-
AB2N10_RS01000	-	225248..225391 (+)	144	WP_369434186.1	hypothetical protein	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 42455.65 Da
• Isoelectric Point: 6.1425

>NTDB_id=1022225 AB2N10_RS00975 WP_354624697.1 219517..220677(+) (pilU) [Psychromonas sp. MME1]
MMEFEEMLTILSSRDGSDLYLSTGAPASAKFQGVLEALTDSPLKSGEIALIADAIMDGEQKIAFEQDLEMNLAISISKVG
RFRVNIFKQRNEVSIVARNIKIDIPKFADLRLPEILKDVIMAKRGLVLFIGGTGSGKSTSLAALIDHRNTNSSGHIITIE
DPVEYVHQHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLAISTLHANNANQALDR
IINFFPEERRPQLLSDLGNNLQSFISQRLIPTVDGKRCAAIEVMMGTLTVRDMIKRGEFGLLKEIMEKSKQLGMQTFDGA
LFDLAVEGRISEAEAIKNSDSPTNLALKFKLHHGGVAEHAAENEKKSGGLTLEPLSPKSTDDLSFS

Nucleotide

• Length: 1161 bp

>NTDB_id=1022225 AB2N10_RS00975 WP_354624697.1 219517..220677(+) (pilU) [Psychromonas sp. MME1]
ATGATGGAATTTGAAGAGATGCTAACGATCTTGTCGAGTCGCGATGGCTCGGATCTTTATTTGTCCACTGGGGCGCCTGC
TTCCGCAAAATTTCAAGGTGTATTAGAAGCACTTACCGATAGCCCTTTAAAGTCCGGTGAAATTGCATTGATCGCAGATG
CCATAATGGATGGCGAACAAAAAATAGCCTTTGAGCAAGATTTAGAAATGAACTTAGCGATCTCCATTTCCAAGGTGGGG
CGTTTCCGTGTCAATATTTTTAAACAACGTAATGAAGTGTCGATCGTTGCCCGTAATATTAAAATTGATATCCCCAAATT
TGCCGATTTACGCTTACCAGAAATTCTCAAAGATGTGATCATGGCAAAACGAGGCTTAGTGCTCTTTATTGGTGGCACGG
GATCGGGTAAATCAACCTCACTAGCTGCATTGATTGATCACCGTAATACGAACTCTTCTGGGCATATTATTACCATTGAA
GATCCCGTTGAGTATGTCCATCAGCATAAAAAAAGTATTATTAATCAACGCGAAGTTGGGGTTGATACGCGTAGCTTTCA
TGCCGCATTAAAAAATACATTACGTCAAGCACCCGATGTTATTTTAATTGGTGAAATACGTGATAGAGAAACCATGGAGC
ATGCTTTAGCGTTCGCCGAAACAGGGCACTTAGCGATATCGACGTTACATGCGAATAATGCGAACCAAGCATTAGATCGT
ATTATTAACTTTTTCCCAGAAGAGCGTCGTCCACAATTGCTAAGCGACTTAGGTAATAATTTACAATCATTTATCTCACA
GCGCTTAATTCCAACCGTTGATGGTAAACGATGTGCTGCGATAGAAGTGATGATGGGCACCTTAACGGTACGCGACATGA
TCAAACGTGGTGAGTTCGGTTTATTAAAAGAGATAATGGAAAAATCTAAACAGCTCGGTATGCAAACCTTTGATGGGGCA
TTATTTGATTTAGCAGTAGAAGGGCGTATCTCCGAAGCGGAAGCAATAAAAAACTCGGATTCACCGACCAACTTAGCGCT
TAAATTTAAACTACATCATGGCGGTGTTGCCGAACATGCTGCTGAGAATGAGAAAAAAAGTGGCGGCTTAACCTTAGAGC
CATTATCACCAAAATCAACGGATGATTTGAGTTTTTCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	52.279	96.632	0.505
pilU	Acinetobacter baylyi ADP1	52.557	91.192	0.479
pilU	Vibrio cholerae strain A1552	48.023	91.71	0.44
pilT	Pseudomonas aeruginosa PAK	41.159	89.378	0.368
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.173	89.637	0.36