Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AB1S65_RS07230 Genome accession   NZ_CP160474
Coordinates   1762991..1764058 (+) Length   355 a.a.
NCBI ID   WP_280947729.1    Uniprot ID   -
Organism   Saccharophagus degradans strain HMH0004     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1757991..1769058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB1S65_RS07220 (AB1S65_07215) rpmE 1759964..1760176 (+) 213 WP_011469170.1 50S ribosomal protein L31 -
  AB1S65_RS07225 (AB1S65_07220) - 1760294..1762834 (-) 2541 WP_280947728.1 penicillin-binding protein 1A -
  AB1S65_RS07230 (AB1S65_07225) pilM 1762991..1764058 (+) 1068 WP_280947729.1 pilus assembly protein PilM Machinery gene
  AB1S65_RS07235 (AB1S65_07230) - 1764060..1764620 (+) 561 WP_280947730.1 PilN domain-containing protein -
  AB1S65_RS07240 (AB1S65_07235) - 1764623..1765237 (+) 615 WP_280947731.1 type 4a pilus biogenesis protein PilO -
  AB1S65_RS07245 (AB1S65_07240) - 1765246..1765791 (+) 546 WP_280947732.1 pilus assembly protein PilP -
  AB1S65_RS07250 (AB1S65_07245) pilQ 1765796..1767982 (+) 2187 WP_280947733.1 type IV pilus secretin PilQ Machinery gene
  AB1S65_RS07255 (AB1S65_07250) aroK 1767985..1768494 (+) 510 WP_280947734.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 38705.92 Da        Isoelectric Point: 4.2610

>NTDB_id=1021456 AB1S65_RS07230 WP_280947729.1 1762991..1764058(+) (pilM) [Saccharophagus degradans strain HMH0004]
MSILNLFTKKKNPMLGLDVSSTSVKLLELSRHGDSYRVENYAVRALPANSVVEKNINDVEAVAQAVKAVVQTSRTKIKDA
AVAVAGSSVITKVIDMPADLSEEDMELQISIEADQYIPYPLEEVAIDFDIQGPSAKSEDRVDVLLAACRRENVDIRATML
ELADLNPKVIDVEAYTMERAFALVREQLEDQDEQVVAVLDIGATMTTLSVLVDGKTIYTREQLFGGKQLTEEIQRRYGLS
AEEAGLAKKQGGLPDDYEPEVLEPFKDAVVQQITRSLQFFFSSSQYNDVDHIVIAGGVASLEGLSNLVEEKLGTSVTIAN
PFANMSVSSRVNATALSNDAPSLMIAVGLALRSFE

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=1021456 AB1S65_RS07230 WP_280947729.1 1762991..1764058(+) (pilM) [Saccharophagus degradans strain HMH0004]
ATGAGTATTCTCAACTTGTTCACTAAGAAAAAGAACCCCATGCTGGGATTGGACGTGAGTTCAACGTCCGTAAAGCTCTT
GGAGCTTAGCCGGCATGGAGATAGCTATCGGGTTGAGAATTACGCCGTGCGCGCTCTTCCTGCCAACTCCGTGGTCGAGA
AAAACATCAACGATGTGGAGGCTGTCGCGCAGGCGGTCAAAGCTGTTGTGCAAACCTCTCGTACCAAAATCAAAGATGCT
GCCGTCGCGGTCGCAGGTTCGTCGGTTATCACCAAGGTGATCGACATGCCAGCGGATCTGAGCGAAGAAGATATGGAGTT
GCAGATATCTATCGAGGCCGACCAATATATTCCCTACCCCTTAGAAGAGGTCGCCATTGACTTCGATATTCAGGGGCCCT
CAGCCAAAAGTGAAGACCGTGTCGATGTGCTTTTGGCTGCGTGCCGAAGGGAAAACGTCGATATTCGCGCAACCATGCTA
GAGCTTGCCGATCTCAACCCCAAAGTGATTGATGTAGAAGCCTATACCATGGAGCGCGCATTTGCGCTTGTGCGAGAGCA
ACTAGAAGATCAAGACGAACAGGTTGTGGCGGTACTCGATATTGGGGCTACCATGACGACGCTAAGTGTGTTGGTCGACG
GGAAAACAATATACACGCGCGAGCAGTTATTCGGCGGTAAGCAGCTTACGGAAGAAATTCAGCGTCGATACGGCCTTTCT
GCTGAAGAGGCTGGGTTGGCTAAGAAACAAGGTGGCTTACCTGATGATTATGAGCCAGAAGTGTTGGAGCCTTTCAAGGA
TGCTGTGGTTCAACAGATTACACGTTCTTTGCAGTTCTTCTTTTCCTCGAGCCAGTATAACGATGTAGACCATATCGTTA
TTGCGGGCGGTGTTGCTAGCTTGGAGGGATTGTCTAACCTAGTAGAAGAAAAGTTAGGCACTTCGGTTACCATCGCAAAT
CCATTTGCCAATATGTCTGTTTCCTCTCGCGTTAACGCAACCGCGCTATCGAATGATGCGCCGTCGCTAATGATCGCAGT
TGGTTTGGCGCTAAGGAGCTTCGAATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-354)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.091

99.437

0.558
  comM Acinetobacter nosocomialis M2

56.091

99.437

0.558
  comM Acinetobacter baylyi ADP1

54.108

99.437

0.538
  pilM Legionella pneumophila strain ERS1305867

45.07

100

0.451

Multiple sequence alignment