Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABXU54_RS23485 Genome accession   NZ_CP159887
Coordinates   5038802..5040505 (+) Length   567 a.a.
NCBI ID   WP_354680853.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain P113     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5033802..5045505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXU54_RS23465 nadC 5036259..5037107 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ABXU54_RS23475 - 5037272..5037970 (-) 699 WP_023121720.1 hypothetical protein -
  ABXU54_RS23480 - 5038034..5038573 (-) 540 WP_003116248.1 pilin -
  ABXU54_RS23485 pilB 5038802..5040505 (+) 1704 WP_354680853.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABXU54_RS23490 pilC 5040509..5041726 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  ABXU54_RS23495 pilD 5041729..5042601 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ABXU54_RS23500 coaE 5042598..5043209 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  ABXU54_RS23505 yacG 5043206..5043406 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ABXU54_RS23510 - 5043443..5043652 (-) 210 WP_003094660.1 hypothetical protein -
  ABXU54_RS23515 - 5043758..5044447 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  ABXU54_RS23520 - 5044444..5044914 (-) 471 WP_003094664.1 hypothetical protein -
  ABXU54_RS23525 - 5044911..5045336 (-) 426 WP_003116252.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62709.67 Da        Isoelectric Point: 5.7065

>NTDB_id=1016598 ABXU54_RS23485 WP_354680853.1 5038802..5040505(+) (pilB) [Pseudomonas aeruginosa strain P113]
MNDTIQLSGLARQLVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTRHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1016598 ABXU54_RS23485 WP_354680853.1 5038802..5040505(+) (pilB) [Pseudomonas aeruginosa strain P113]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTATTGGATGCGATCAGAGGCGGCTCCTCTGATCTGCATTTCGAGCCCTATGAAAAGATTTACC
GTGTACGTTTCCGTACCGATGGCATGCTCCATGAGGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTAATGGCAGGCCTGGACATTTCCGAACGGCGTAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTTAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGTATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCAGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGATCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.58
  pilB Acinetobacter baylyi ADP1

57.092

99.471

0.568
  pilB Legionella pneumophila strain ERS1305867

54.93

100

0.55
  pilF Neisseria gonorrhoeae MS11

50.266

99.295

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

51.763

95.062

0.492
  pilB Vibrio cholerae strain A1552

50

97.002

0.485
  pilB Vibrio campbellii strain DS40M4

50.647

95.414

0.483
  pilF Thermus thermophilus HB27

38.407

99.647

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40

93.474

0.374

Multiple sequence alignment