Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   N027_RS11725 Genome accession   NZ_CP159278
Coordinates   2611757..2612926 (+) Length   389 a.a.
NCBI ID   WP_024657668.1    Uniprot ID   A0AAU8MG82
Organism   Pseudomonas syringae USA007     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2606757..2617926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N027_RS11715 (N027_11715) pgm 2609184..2610830 (+) 1647 WP_024656102.1 phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent) -
  N027_RS11720 (N027_11720) - 2611327..2611602 (-) 276 WP_003373489.1 peptidylprolyl isomerase -
  N027_RS11725 (N027_11725) pilU 2611757..2612926 (+) 1170 WP_024657668.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  N027_RS11730 (N027_11730) - 2613467..2615044 (-) 1578 WP_024657669.1 ABC transporter ATP-binding protein -
  N027_RS11735 (N027_11735) - 2615046..2616068 (-) 1023 WP_005618488.1 ABC transporter permease -
  N027_RS11740 (N027_11740) - 2616068..2617132 (-) 1065 WP_011104338.1 microcin C ABC transporter permease YejB -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 42685.81 Da        Isoelectric Point: 5.8676

>NTDB_id=1013018 N027_RS11725 WP_024657668.1 2611757..2612926(+) (pilU) [Pseudomonas syringae USA007]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLSSETLKPGEVAVVAQGLMDEEQKLEFVRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSENGVTSLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=1013018 N027_RS11725 WP_024657668.1 2611757..2612926(+) (pilU) [Pseudomonas syringae USA007]
ATGGATTTTCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCTGACCTTTATCTTTCTACCGGAGCACCGCCGTG
TGCCAAGTTCAACGGCGTGCTCAAGCCGCTGAGCAGCGAAACCCTCAAGCCTGGCGAAGTGGCGGTGGTTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAGTTTGTCCGTGAACTGGAGATGAACCTCGCGGTGTCATTGGCAGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCCATTGTCGCGCGTAACATCAAGCTGGACATTCCGCGCTTTGA
GGACCTTTTCCTGCCGCCGGTGTTGCTTGACGTGATCATGGAAAAACACGGTCTGGTGCTGTTTGTCGGCGCCACCGGCT
CGGGTAAATCGACCTCGCTGGCGGCACTGATCGATTACCGTAACCGCAATGCCAGCGGGCACATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAATCCATCGTCAATCAGCGTGAGGTCGGCGTCGACACCCGCAGTTTTCGCGC
GGCACTGAAAAACACCTTGCGTCAGGCTCCGGATGTGATTCTGATCGGCGAGATCCGCGATCGCGAAACGATGGAGCACG
CGCTGGCATTCGCCGACACCGGCCACCTGGCGATTTCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGAATC
ATCAACTTCTTCCCGGAAGAGCGGCGTGCGCAGCTGTTGCACGACCTTGGCAACAACCTCAAGGCGTTCGTGTCTCAGCG
GCTGGTAAAGACGCCTGATGGAAAACGGCGCGCGGCGGTGGAGGTAATGATGGGCACGCCGACCATCCGCGATCTGATCC
AGCGCAACGAACTGACCGAGCTCAAGGGCATCATGGAAAAGTCTGGCAGCCTCGGGATGCAGACGTTCGATACCGCACTG
TTCAACCTGGCCGTCGAAGGTGCGATCAGCGAGGAAGAGGCGCTGAAGAATGCCGACTCGCAAAACAACGTGCGTCTGCG
CCTCAAGCTGCACAGCGAAAATGGAGTGACCAGCCTGACCACGCCGCCCCCGGCACCGACCGGCAGCAGCACGGCCAGCA
CCGCCGAGTGGGGCCTGGTCGACGATGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.072

99.486

0.558
  pilU Acinetobacter baylyi ADP1

52.113

91.26

0.476
  pilU Vibrio cholerae strain A1552

51.143

89.974

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.164

98.458

0.386
  pilT Pseudomonas aeruginosa PAK

41.399

88.175

0.365

Multiple sequence alignment