Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LLUC023_RS11620 Genome accession   NZ_CP158368
Coordinates   2252798..2253283 (-) Length   161 a.a.
NCBI ID   WP_242515029.1    Uniprot ID   -
Organism   Lactococcus cremoris strain UC023     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2226175..2253295 2252798..2253283 within 0
IScluster/Tn 2251576..2255090 2252798..2253283 within 0


Gene organization within MGE regions


Location: 2226175..2255090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLUC023_RS11415 (LLUC023_11425) - 2226175..2227047 (+) 873 WP_014573331.1 RluA family pseudouridine synthase -
  LLUC023_RS11425 (LLUC023_11435) - 2228529..2229419 (-) 891 WP_396419884.1 IS982-like element IS982B family transposase -
  LLUC023_RS11430 (LLUC023_11440) - 2229584..2230393 (-) 810 WP_011677177.1 metal ABC transporter permease -
  LLUC023_RS11435 (LLUC023_11445) - 2230386..2231123 (-) 738 WP_011677178.1 metal ABC transporter ATP-binding protein -
  LLUC023_RS11440 (LLUC023_11450) - 2231302..2232144 (-) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LLUC023_RS11445 (LLUC023_11455) - 2232141..2232578 (-) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  LLUC023_RS11450 (LLUC023_11460) comGG 2232658..2232957 (-) 300 WP_011677181.1 competence type IV pilus minor pilin ComGG Machinery gene
  LLUC023_RS11455 (LLUC023_11465) comGF 2232981..2233244 (-) 264 WP_021211201.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLUC023_RS11460 (LLUC023_11470) istB 2233327..2234085 (-) 759 WP_003331414.1 IS21-like element IS712 family helper ATPase IstB -
  LLUC023_RS11465 (LLUC023_11475) istA 2234097..2235320 (-) 1224 WP_003331415.1 IS21-like element IS712 family transposase -
  LLUC023_RS11470 (LLUC023_11480) - 2235376..2235498 (-) 123 WP_021211203.1 hypothetical protein -
  LLUC023_RS11475 (LLUC023_11485) - 2235491..2235901 (-) 411 WP_011676523.1 terminase -
  LLUC023_RS11480 (LLUC023_11490) - 2236343..2236765 (-) 423 WP_014573151.1 RinA family protein -
  LLUC023_RS11485 (LLUC023_11495) - 2236843..2237151 (-) 309 WP_021211204.1 hypothetical protein -
  LLUC023_RS11490 (LLUC023_11500) - 2237402..2237740 (-) 339 WP_021211205.1 DUF1140 family protein -
  LLUC023_RS11495 (LLUC023_11505) dut 2237741..2238160 (-) 420 WP_282667355.1 dUTP diphosphatase -
  LLUC023_RS11500 (LLUC023_11510) - 2238157..2238753 (-) 597 WP_151318410.1 DUF1642 domain-containing protein -
  LLUC023_RS11505 (LLUC023_11515) - 2238746..2238952 (-) 207 WP_011676933.1 DUF1125 domain-containing protein -
  LLUC023_RS11510 (LLUC023_11520) - 2238966..2239490 (-) 525 WP_205288144.1 hypothetical protein -
  LLUC023_RS11515 (LLUC023_11525) - 2239596..2239835 (-) 240 WP_021211207.1 DUF1031 domain-containing protein -
  LLUC023_RS11520 (LLUC023_11530) - 2239836..2240255 (-) 420 WP_021165554.1 hypothetical protein -
  LLUC023_RS11525 (LLUC023_11535) - 2240245..2240466 (-) 222 WP_021211208.1 hypothetical protein -
  LLUC023_RS11530 (LLUC023_11540) - 2240447..2241256 (-) 810 WP_021211209.1 helix-turn-helix domain-containing protein -
  LLUC023_RS11535 (LLUC023_11545) - 2241483..2242550 (-) 1068 WP_021211210.1 DUF1351 domain-containing protein -
  LLUC023_RS11540 (LLUC023_11550) bet 2242552..2243289 (-) 738 WP_021211211.1 phage recombination protein Bet -
  LLUC023_RS11545 (LLUC023_11555) - 2243376..2244266 (-) 891 WP_332371208.1 IS982-like element ISLll1 family transposase -
  LLUC023_RS11550 (LLUC023_11560) - 2244418..2244633 (-) 216 WP_010905687.1 DUF1408 domain-containing protein -
  LLUC023_RS11555 (LLUC023_11565) - 2244736..2245014 (+) 279 WP_011834777.1 hypothetical protein -
  LLUC023_RS11560 (LLUC023_11570) - 2244969..2245202 (-) 234 WP_011834776.1 hypothetical protein -
  LLUC023_RS11565 (LLUC023_11575) - 2245547..2246290 (-) 744 WP_031298611.1 ORF6C domain-containing protein -
  LLUC023_RS11570 (LLUC023_11580) - 2246305..2246535 (-) 231 WP_011676017.1 hypothetical protein -
  LLUC023_RS11575 (LLUC023_11585) - 2246825..2247172 (+) 348 WP_031298613.1 XRE family transcriptional regulator -
  LLUC023_RS11580 (LLUC023_11590) - 2247201..2247764 (+) 564 WP_021211213.1 hypothetical protein -
  LLUC023_RS11585 (LLUC023_11595) - 2247776..2247976 (+) 201 WP_021211214.1 hypothetical protein -
  LLUC023_RS11590 (LLUC023_11600) - 2248052..2248489 (+) 438 WP_259751086.1 DUF6978 family protein -
  LLUC023_RS11595 (LLUC023_11605) - 2248515..2249306 (+) 792 WP_205288149.1 DUF1829 domain-containing protein -
  LLUC023_RS11600 (LLUC023_11610) - 2249424..2250881 (+) 1458 WP_205288150.1 recombinase family protein -
  LLUC023_RS11605 (LLUC023_11615) comGC 2250878..2251012 (-) 135 WP_021211216.1 hypothetical protein Machinery gene
  LLUC023_RS11610 (LLUC023_11620) comGB 2251030..2251479 (-) 450 WP_021211217.1 type II secretion system F family protein Machinery gene
  LLUC023_RS11615 (LLUC023_11625) - 2251576..2252751 (+) 1176 WP_003138385.1 IS256-like element IS905 family transposase -
  LLUC023_RS11620 (LLUC023_11630) comGB 2252798..2253283 (-) 486 WP_242515029.1 type II secretion system F family protein Machinery gene
  LLUC023_RS11625 (LLUC023_11635) - 2253276..2254097 (-) 822 Protein_2265 ATPase, T2SS/T4P/T4SS family -
  LLUC023_RS11630 (LLUC023_11640) - 2254200..2255090 (+) 891 WP_396419901.1 IS982 family transposase -

Sequence

Protein


Download         Length: 161 a.a.        Molecular weight: 17932.49 Da        Isoelectric Point: 10.0696

>NTDB_id=1011031 LLUC023_RS11620 WP_242515029.1 2252798..2253283(-) (comGB) [Lactococcus cremoris strain UC023]
MNNGFHLGEVINFLALSKLVEKEFVFKMHQGLSSGNSLSEILDSLSFSKNVVTQLALVEVHGNLVGTMQLVELHLKKQLK
VKKKLIEVATYPVVLLVFLIGIMWGLKNYLLPQIDKGNNFATLLINHLPLVFFSVGALIVLLTSLSIIIFKHLSALTNFT
F

Nucleotide


Download         Length: 486 bp        

>NTDB_id=1011031 LLUC023_RS11620 WP_242515029.1 2252798..2253283(-) (comGB) [Lactococcus cremoris strain UC023]
TTGAATAATGGTTTCCATTTGGGAGAAGTTATCAATTTTTTAGCTTTATCAAAATTAGTAGAAAAAGAGTTTGTATTCAA
AATGCACCAAGGTCTCAGTTCTGGAAATTCATTATCTGAAATTTTAGATAGTCTCTCTTTTTCTAAAAATGTTGTGACAC
AACTTGCTTTGGTAGAAGTGCATGGGAATTTAGTAGGAACCATGCAATTGGTAGAACTGCATTTAAAAAAGCAGCTTAAG
GTCAAAAAGAAATTAATTGAAGTGGCAACTTATCCAGTTGTATTGTTGGTATTTCTCATTGGAATTATGTGGGGCTTGAA
AAATTATTTATTACCTCAAATTGATAAGGGAAATAATTTTGCTACTTTATTAATTAATCATTTACCGCTAGTATTTTTTT
CTGTTGGTGCTTTAATCGTTTTACTGACAAGTTTATCAATAATAATATTCAAACATCTTTCAGCACTCACTAATTTTACT
TTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(21-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

96.273

100

0.963
  comGB/cglB Streptococcus mitis SK321

49.686

98.758

0.491
  comYB Streptococcus gordonii str. Challis substr. CH1

49.375

99.379

0.491
  comGB/cglB Streptococcus pneumoniae TIGR4

48.428

98.758

0.478
  comGB/cglB Streptococcus pneumoniae Rx1

48.428

98.758

0.478
  comGB/cglB Streptococcus pneumoniae D39

48.428

98.758

0.478
  comGB/cglB Streptococcus pneumoniae R6

48.428

98.758

0.478
  comGB/cglB Streptococcus mitis NCTC 12261

47.799

98.758

0.472
  comYB Streptococcus mutans UA140

48.387

96.273

0.466
  comYB Streptococcus mutans UA159

47.742

96.273

0.46

Multiple sequence alignment