Detailed information NTG1009278

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ABL091_RS12160
• Genome accession: NZ_CP157944
• Coordinates: 2521312..2521926 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain B6
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2516312..2526926

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABL091_RS12130 (ABL091_12125)	rpsT	2516615..2516881 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ABL091_RS12135 (ABL091_12130)	holA	2516898..2517939 (-)	1042	Protein_2342	DNA polymerase III subunit delta	-
ABL091_RS12140 (ABL091_12135)	-	2517979..2518130 (-)	152	Protein_2343	hypothetical protein	-
ABL091_RS12145 (ABL091_12140)	-	2518171..2518305 (+)	135	WP_003152870.1	YqzM family protein	-
ABL091_RS12150 (ABL091_12145)	comEC	2518324..2520675 (-)	2352	WP_089072447.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ABL091_RS12155 (ABL091_12150)	-	2520676..2521245 (-)	570	WP_003152868.1	ComE operon protein 2	-
ABL091_RS12160 (ABL091_12155)	comEA	2521312..2521926 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
ABL091_RS12165 (ABL091_12160)	comER	2521985..2522806 (+)	822	WP_014418420.1	late competence protein ComER	-
ABL091_RS12170 (ABL091_12165)	-	2522875..2523612 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
ABL091_RS12175 (ABL091_12170)	rsfS	2523609..2523965 (-)	357	WP_007408260.1	ribosome silencing factor	-
ABL091_RS12180 (ABL091_12175)	yqeK	2523983..2524543 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ABL091_RS12185 (ABL091_12180)	-	2524533..2525102 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ABL091_RS12190 (ABL091_12185)	yhbY	2525113..2525403 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ABL091_RS12195 (ABL091_12190)	aroE	2525397..2526239 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=1009278 ABL091_RS12160 WP_014418419.1 2521312..2521926(-) (comEA) [Bacillus velezensis strain B6]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1009278 ABL091_RS12160 WP_014418419.1 2521312..2521926(-) (comEA) [Bacillus velezensis strain B6]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363