Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABL846_RS21200 Genome accession   NZ_CP157632
Coordinates   4435330..4437063 (+) Length   577 a.a.
NCBI ID   WP_015866994.1    Uniprot ID   -
Organism   Variovorax paradoxus strain 295MFChir4.1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4430330..4442063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL846_RS21165 (ABL846_21155) - 4430395..4430925 (+) 531 WP_015866988.1 CNP1-like family protein -
  ABL846_RS21170 (ABL846_21160) proB 4430991..4432130 (-) 1140 WP_015866989.1 glutamate 5-kinase -
  ABL846_RS21175 (ABL846_21165) cgtA 4432169..4433257 (-) 1089 WP_015866990.1 Obg family GTPase CgtA -
  ABL846_RS21180 (ABL846_21170) rpmA 4433328..4433585 (-) 258 WP_015866991.1 50S ribosomal protein L27 -
  ABL846_RS21185 (ABL846_21175) rplU 4433598..4433909 (-) 312 WP_015866992.1 50S ribosomal protein L21 -
  ABL846_RS21190 (ABL846_21180) - 4434098..4435090 (+) 993 WP_021008896.1 polyprenyl synthetase family protein -
  ABL846_RS21200 (ABL846_21190) pilB 4435330..4437063 (+) 1734 WP_015866994.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABL846_RS21205 (ABL846_21195) pilC 4437093..4438322 (+) 1230 WP_015866995.1 type II secretion system F family protein Machinery gene
  ABL846_RS21210 (ABL846_21200) - 4438322..4439287 (+) 966 WP_015866996.1 A24 family peptidase -
  ABL846_RS21215 (ABL846_21205) coaE 4439298..4439903 (+) 606 WP_015866997.1 dephospho-CoA kinase -
  ABL846_RS21220 (ABL846_21210) - 4439982..4441475 (+) 1494 WP_027731016.1 MBOAT family O-acyltransferase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62668.92 Da        Isoelectric Point: 6.2073

>NTDB_id=1007875 ABL846_RS21200 WP_015866994.1 4435330..4437063(+) (pilB) [Variovorax paradoxus strain 295MFChir4.1]
MAAAELPVKENTQIALPGLARALVSAGKLPAKTAEDIYQKSLSGRTSFIAELTGSGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCHAYRIVVLSKRNNRLIVATADPSDQQAVEKIKFASQMGVDWVIAEYDKLSRMIEAAAVTAAETI
NSIVGAEFEFDDVSADTSGDANEQAIAEVEDAPVVRFLHKMLLDGVSMRASDIHFEPYEHNYRVRFRIDGELREIASPPT
LIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYDADEKER
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCHVCRAPADMPR
QALLDAGFKEEQLDGTWKPYRPVGCAACSNGYKGRVGIYQVMPISEAIQAIILRDGSALDIARQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1007875 ABL846_RS21200 WP_015866994.1 4435330..4437063(+) (pilB) [Variovorax paradoxus strain 295MFChir4.1]
ATGGCTGCTGCCGAACTTCCTGTTAAAGAAAACACGCAAATCGCCCTCCCGGGCCTTGCGCGTGCCTTGGTCTCGGCCGG
CAAGCTTCCTGCGAAGACCGCGGAAGACATCTATCAAAAGTCGCTGAGCGGCCGCACCAGCTTCATTGCCGAGCTGACCG
GCAGCGGCGCCGTCTCGGCCGCCGACCTCGCCCACACGCTGTCCAGCGCCTTCGGCGCGCCGCTGCTGGACCTGGACGCC
ATCGACCACCAGCGCCTGCCCAAGGACCTGCTCGACCCGAAGCTGTGCCACGCCTACCGGATCGTGGTTCTCAGCAAGCG
CAACAACCGTCTCATCGTTGCAACGGCCGACCCTTCGGACCAGCAGGCGGTGGAAAAGATCAAGTTCGCCTCCCAGATGG
GCGTGGACTGGGTCATTGCCGAATACGACAAGCTCTCGCGCATGATCGAAGCCGCCGCGGTCACTGCGGCGGAAACCATC
AACAGCATCGTCGGCGCCGAGTTCGAGTTCGACGACGTCTCGGCGGACACCTCGGGCGATGCCAACGAACAGGCCATCGC
CGAGGTCGAGGACGCGCCGGTCGTGCGCTTCCTGCACAAGATGCTGCTCGACGGTGTCAGCATGCGGGCCTCGGACATCC
ACTTCGAGCCCTACGAGCACAACTACCGGGTGCGCTTTCGCATCGACGGCGAGCTGCGCGAGATCGCGAGCCCGCCCACG
CTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATCTCGCGGCTCGACATTTCGGAGAAGCGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGGCCCGACCGCGTGATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAGA
TCGTGATCCGTATCCTGGACCCGAGCAGCGCGCGCCTGGGCATCGACGCACTGGGCTACGACGCCGACGAAAAGGAGCGC
CTGCTGCATGCGATCGGCCGCCCCTATGGCATGGTGCTGGTGACGGGCCCCACGGGTTCGGGCAAGACGGTGTCGCTCTA
CACCTGCCTGAACCTGCTGAACCAGCCCGGCGTCAACATCGCCACGGCGGAAGACCCGTCCGAAATCAACCTGCCCGGCG
TGAACCAGGTGAACGTCAACGAGCGCGCCGGGCTCACCTTCGCGGCGGCGCTGCGCGCCTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACGGGCCACCTGGTGCT
CTCGACGCTGCACACCAACGACGCGCCGACCACGCTCACGCGCATGCGCAACATGGGCATTGCGCCGTTCAACATTGCCT
CCAGCGTGATCCTGATCACGGCGCAGCGGCTCGCGCGGCGCCTGTGCCACGTATGCCGCGCGCCGGCCGACATGCCGCGC
CAGGCGCTGCTGGATGCGGGCTTCAAGGAAGAACAGCTCGACGGTACCTGGAAGCCCTATCGGCCGGTCGGCTGCGCGGC
GTGCAGCAACGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATCTCGGAAGCCATCCAGGCGATCATCCTGC
GCGACGGCAGCGCACTCGACATCGCACGCCAGTCCGAGGCCGAGGGCGTGCGCTCGCTGCGGCAATCGGGCCTGCGAAAA
GTCATGCAAGGGCTCACCTCACTCGAAGAAGTGGTGGCGGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.697

98.44

0.529
  pilB Acinetobacter baylyi ADP1

53.654

97.227

0.522
  pilF Neisseria gonorrhoeae MS11

52.22

97.574

0.51
  pilB Legionella pneumophila strain ERS1305867

49.204

97.92

0.482
  pilB Vibrio cholerae strain A1552

46.964

97.054

0.456
  pilB Vibrio campbellii strain DS40M4

45.664

97.92

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.374

97.4

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.963

94.107

0.376
  pilF Thermus thermophilus HB27

37.478

98.96

0.371

Multiple sequence alignment