Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABL851_RS32665 Genome accession   NZ_CP157614
Coordinates   6917856..6919589 (-) Length   577 a.a.
NCBI ID   WP_093198333.1    Uniprot ID   A0A1H7MFN6
Organism   Variovorax sp. NFACC26     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6912856..6924589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL851_RS32645 (ABL851_32590) - 6913453..6914946 (-) 1494 WP_093302402.1 MBOAT family O-acyltransferase -
  ABL851_RS32650 (ABL851_32595) coaE 6915016..6915627 (-) 612 WP_176947478.1 dephospho-CoA kinase -
  ABL851_RS32655 (ABL851_32600) - 6915635..6916600 (-) 966 WP_093302399.1 prepilin peptidase -
  ABL851_RS32660 (ABL851_32605) pilC 6916600..6917829 (-) 1230 WP_093302396.1 type II secretion system F family protein Machinery gene
  ABL851_RS32665 (ABL851_32610) pilB 6917856..6919589 (-) 1734 WP_093198333.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABL851_RS32675 (ABL851_32620) - 6919834..6920826 (-) 993 WP_093302393.1 polyprenyl synthetase family protein -
  ABL851_RS32680 (ABL851_32625) rplU 6921011..6921322 (+) 312 WP_057592097.1 50S ribosomal protein L21 -
  ABL851_RS32685 (ABL851_32630) rpmA 6921335..6921595 (+) 261 WP_093057448.1 50S ribosomal protein L27 -
  ABL851_RS32690 (ABL851_32635) cgtA 6921667..6922743 (+) 1077 WP_093302390.1 Obg family GTPase CgtA -
  ABL851_RS32695 (ABL851_32640) proB 6922820..6923962 (+) 1143 WP_093302387.1 glutamate 5-kinase -
  ABL851_RS32700 (ABL851_32645) - 6924032..6924559 (-) 528 WP_093302384.1 CNP1-like family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62518.64 Da        Isoelectric Point: 5.3252

>NTDB_id=1007716 ABL851_RS32665 WP_093198333.1 6917856..6919589(-) (pilB) [Variovorax sp. NFACC26]
MAAAELPVKEPSQIALPGLARALVSAGKLPAKAAEDIYQKSLSGRTSFIAELTGSGAVSAADLAHTLSTAFGAPLLDLDA
IDPQRLPKDLVDQKLCHAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAVSAAETI
NSIVGSEFEFDEVSAETASESNDQAIAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHNYRVRFRVDGELREVASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARLGIDALGYDADEKAR
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTVCKLPADIPR
ETLLEAGFQEEELDGSWKPYRPVGCSACSSGYKGRVGIYQVMPISEAIQEIILRDGSALDIATQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVACTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1007716 ABL851_RS32665 WP_093198333.1 6917856..6919589(-) (pilB) [Variovorax sp. NFACC26]
ATGGCCGCTGCCGAACTTCCTGTTAAAGAACCCTCGCAGATTGCCCTCCCAGGGCTTGCGCGTGCCCTGGTTTCGGCCGG
CAAGCTCCCTGCCAAGGCGGCCGAGGATATCTACCAGAAGTCCCTCAGTGGCCGCACCAGCTTCATCGCGGAGCTGACCG
GCTCCGGCGCCGTCTCGGCAGCCGACCTCGCTCACACCCTGTCCACGGCCTTCGGCGCCCCGCTGCTCGACCTGGACGCC
ATCGACCCCCAGCGCCTGCCGAAGGATCTGGTCGACCAGAAGCTGTGCCATGCGTACCGCATCGTCGTTCTCAGCAAGCG
CAACAACCGTCTGATCGTTGCAACAGCCGATCCATCCGACCAGCAGGCGGCGGAGAAGATCAAGTTCGCCTCCCAGATGG
GCGTCGACTGGGTCATCGCCGAATACGACAAGCTGTCGCGAATGATCGAAGCCGCCGCCGTCAGCGCTGCGGAAACCATC
AACAGCATCGTCGGCAGTGAGTTCGAATTCGACGAGGTCTCTGCCGAGACGGCCAGCGAAAGCAACGACCAGGCAATCGC
CGAAGTCGAGGACGCGCCGGTCGTTCGCTTCCTGCACAAGATGCTGCTCGACGCCGTCAGCATGCGCGCCTCGGACATCC
ACTTCGAGCCGTACGAACACAACTACCGCGTGCGCTTCCGGGTCGACGGCGAGTTGCGCGAGGTTGCCAGCCCACCCACC
GTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATTTCCAGGCTCGACATCTCCGAGAAACGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGCCCCGACCGCGTGATCGACTTCCGTGTGAGCACGCTGCCCACGCTGTTCGGCGAAAAGA
TCGTCGTTCGTATTCTCGACCCGAGCAGCGCACGACTGGGCATCGACGCCCTCGGCTACGACGCGGACGAAAAGGCACGA
CTGCTGCATGCCATCGGCCGGCCTTACGGCATGGTGCTGGTCACCGGCCCCACGGGCTCCGGCAAGACGGTGTCGCTCTA
CACCTGCCTGAACCTGCTGAACCAGCCCGGCGTCAACATCGCGACGGCGGAAGATCCGTCGGAAATCAACCTGCCCGGCG
TCAACCAGGTCAACGTCAATGAACGCGCGGGCCTGACTTTCGCGGCCGCGCTGCGCGCCTTCCTGCGCCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACGGGTCACCTGGTGCT
CTCCACGCTGCACACCAACGATGCGCCGACCACGCTCACGCGGATGCGCAACATGGGCATCGCGCCGTTCAACATCGCAT
CGAGCGTGATCCTGATCACGGCGCAGCGCCTCGCGCGGCGCCTGTGCACTGTCTGCAAGCTGCCGGCCGACATCCCGCGC
GAAACACTGCTCGAGGCAGGCTTCCAGGAAGAAGAGCTGGACGGCTCCTGGAAGCCCTACCGCCCCGTCGGCTGCTCCGC
CTGCAGCAGCGGCTACAAGGGCCGCGTCGGCATCTATCAGGTGATGCCGATCTCGGAGGCGATCCAGGAAATCATCCTGC
GCGACGGCAGCGCACTCGACATCGCCACGCAGTCGGAAGCCGAGGGCGTGCGTTCGCTGCGCCAGTCGGGCCTGCGCAAG
GTCATGCAAGGTCTCACTTCGCTGGAAGAAGTGGTGGCCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H7MFN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.338

97.4

0.539
  pilB Acinetobacter baylyi ADP1

54.804

97.4

0.534
  pilF Neisseria gonorrhoeae MS11

52.753

97.574

0.515
  pilB Legionella pneumophila strain ERS1305867

49.464

97.054

0.48
  pilB Vibrio cholerae strain A1552

47.794

94.281

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

45.781

96.534

0.442
  pilB Vibrio campbellii strain DS40M4

45.276

97.227

0.44
  pilF Thermus thermophilus HB27

38.529

98.96

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.11

94.627

0.38

Multiple sequence alignment