Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   G3R42_RS27960 Genome accession   NZ_LR739068
Coordinates   6029462..6030085 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PcyII-29 isolate PcyII-29     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6024462..6035085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3R42_RS27940 (PERCYII29_5598) aroB 6025057..6026163 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  G3R42_RS27945 (PERCYII29_5599) aroK 6026213..6026731 (-) 519 WP_003095833.1 shikimate kinase AroK -
  G3R42_RS27950 (PERCYII29_5600) pilQ 6026743..6028887 (-) 2145 WP_003103267.1 type 4a pilus secretin PilQ Machinery gene
  G3R42_RS27955 (PERCYII29_5601) comL/pilP 6028941..6029465 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  G3R42_RS27960 (PERCYII29_5602) pilO 6029462..6030085 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  G3R42_RS27965 (PERCYII29_5604) pilN 6030082..6030677 (-) 596 Protein_5547 type 4a pilus biogenesis protein PilN -
  G3R42_RS27970 (PERCYII29_5605) pilM 6030677..6031741 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  G3R42_RS27975 (PERCYII29_5606) - 6031926..6034397 (+) 2472 WP_003103276.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=1007281 G3R42_RS27960 WP_003103268.1 6029462..6030085(-) (pilO) [Pseudomonas aeruginosa strain PcyII-29 isolate PcyII-29]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=1007281 G3R42_RS27960 WP_003103268.1 6029462..6030085(-) (pilO) [Pseudomonas aeruginosa strain PcyII-29 isolate PcyII-29]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACTTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401

Multiple sequence alignment