Detailed information NTG0100625

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QUD59_RS10400
• Genome accession: NZ_AP027292
• Coordinates: 2247668..2248837 (-)
• Length: 389 a.a.
• NCBI ID: WP_286236898.1
• Uniprot ID: -
• Organism: Neptuniibacter halophilus strain LMG 25378
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2242668..2253837

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QUD59_RS10385	-	2243140..2244069 (+)	930	WP_286236893.1	FAD-binding protein	-
QUD59_RS10390	fadB	2244169..2246316 (+)	2148	WP_286236895.1	fatty acid oxidation complex subunit alpha FadB	-
QUD59_RS10395	fadA	2246329..2247504 (+)	1176	WP_286236897.1	acetyl-CoA C-acyltransferase FadA	-
QUD59_RS10400	pilU	2247668..2248837 (-)	1170	WP_286236898.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
QUD59_RS10405	-	2249011..2249673 (-)	663	WP_286236899.1	YkgJ family cysteine cluster protein	-
QUD59_RS10410	-	2249710..2250087 (-)	378	WP_286236901.1	hypothetical protein	-
QUD59_RS10415	-	2250136..2250918 (-)	783	WP_286236902.1	alpha/beta hydrolase	-
QUD59_RS10420	-	2250930..2251535 (-)	606	WP_286236903.1	TetR/AcrR family transcriptional regulator	-
QUD59_RS10425	-	2251648..2252496 (+)	849	WP_286236904.1	crotonase/enoyl-CoA hydratase family protein	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 43392.47 Da
• Isoelectric Point: 5.0237

>NTDB_id=100625 QUD59_RS10400 WP_286236898.1 2247668..2248837(-) (pilU) [Neptuniibacter halophilus strain LMG 25378]
MDFQDCLTLLAKRDGSDLYLTTGAPPCAKFEGKLTPLSKEPYKPGEIEAIARSLMDEQQQATFEEELEMNLAYSIPKVAR
YRINIFKQRNQFSIVARNIKAEIPQMESLGLPPVLKDVIMEKRGLVLFVGATGSGKSTSLAALIDHRNRNSGGHIITIED
PIEFVHTHRKSLINQREVGVDTRNFHSALKNALRQAPDVILIGEIRDQETMEQCLTYAETGHLAISTLHANNANQAIDRI
INFFPQARHKQIFHDLALNIKAIISQRLIPTIDGKRCAAIEIMLGTSSVQELIMKGDVKAIKEVMAKGGDTTGMQTFDDS
LYRLFKEGKITQDQAIINADSANDMRLRMNLTTEEESAPEEAPQDKPKASYSDDDPLAGMELLDEDEDI

Nucleotide

• Length: 1170 bp

>NTDB_id=100625 QUD59_RS10400 WP_286236898.1 2247668..2248837(-) (pilU) [Neptuniibacter halophilus strain LMG 25378]
ATGGATTTTCAGGATTGCCTCACCCTTCTGGCAAAGCGCGATGGCTCCGACTTATATCTGACCACTGGCGCCCCGCCCTG
CGCTAAGTTTGAAGGCAAACTGACACCCCTGAGCAAAGAACCCTACAAGCCGGGAGAGATCGAAGCGATCGCCCGTTCGC
TGATGGATGAACAGCAACAGGCAACATTTGAAGAGGAGCTGGAGATGAACCTGGCTTACTCCATCCCGAAAGTTGCGCGC
TACCGTATCAACATCTTTAAGCAGCGCAATCAGTTCTCCATCGTGGCGCGTAATATCAAAGCCGAGATTCCCCAGATGGA
ATCATTGGGGCTGCCCCCCGTGCTGAAAGATGTGATCATGGAAAAACGTGGGCTGGTACTGTTTGTAGGGGCGACAGGCT
CAGGTAAATCCACCTCACTGGCGGCCTTGATTGATCACCGCAACCGCAACAGCGGCGGCCATATCATCACCATTGAAGAC
CCGATTGAGTTTGTCCACACCCACCGAAAATCGCTGATCAACCAGCGTGAAGTCGGTGTGGATACCCGTAACTTCCACAG
CGCCCTGAAAAACGCCCTGCGTCAGGCACCGGATGTTATTCTCATCGGTGAGATCCGCGATCAGGAGACCATGGAACAGT
GCCTGACCTACGCTGAAACCGGCCATCTGGCGATCTCAACCCTGCACGCTAACAATGCCAACCAGGCAATCGACCGTATC
ATTAACTTTTTCCCACAGGCCCGGCACAAACAGATCTTTCACGATCTGGCCCTGAACATTAAAGCGATCATCTCGCAGCG
ACTGATCCCGACCATCGACGGTAAGCGTTGCGCCGCTATTGAGATCATGCTCGGTACTTCCAGTGTGCAGGAACTGATCA
TGAAAGGTGACGTTAAAGCCATCAAGGAAGTCATGGCCAAAGGTGGTGATACAACGGGAATGCAAACGTTCGATGACTCC
CTGTACCGTCTGTTCAAAGAGGGCAAGATCACACAGGATCAGGCGATCATCAATGCAGACTCTGCCAATGATATGCGCCT
GCGCATGAACCTGACCACCGAAGAAGAAAGCGCGCCTGAAGAAGCCCCTCAGGATAAGCCAAAAGCCTCCTACAGCGACG
ATGATCCGCTCGCCGGTATGGAGTTGCTCGATGAGGATGAAGATATCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	51.194	96.915	0.496
pilU	Acinetobacter baylyi ADP1	50.693	92.802	0.47
pilU	Vibrio cholerae strain A1552	45.429	89.974	0.409
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.214	86.632	0.383
pilT	Pseudomonas aeruginosa PAK	42.687	86.118	0.368