Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABI424_RS03945 Genome accession   NZ_CP157211
Coordinates   862149..862607 (-) Length   152 a.a.
NCBI ID   WP_012340401.1    Uniprot ID   -
Organism   Histophilus somni strain 23-038383_S91     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 857149..867607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABI424_RS03920 (ABI424_000786) - 858790..859047 (+) 258 WP_178384284.1 DUF5339 domain-containing protein -
  ABI424_RS03925 (ABI424_000787) artM 859153..859836 (-) 684 WP_011608408.1 arginine ABC transporter permease ArtM -
  ABI424_RS03930 (ABI424_000788) artQ 859847..860518 (-) 672 WP_011608407.1 arginine ABC transporter permease ArtQ -
  ABI424_RS03935 (ABI424_000789) - 860523..861248 (-) 726 WP_012340415.1 transporter substrate-binding domain-containing protein -
  ABI424_RS03940 (ABI424_000790) artP 861266..862003 (-) 738 WP_012340409.1 arginine ABC transporter ATP-binding protein ArtP -
  ABI424_RS03945 (ABI424_000791) pilA 862149..862607 (-) 459 WP_012340401.1 prepilin peptidase-dependent pilin Machinery gene
  ABI424_RS03950 (ABI424_000792) ampD 862730..863299 (+) 570 WP_012340392.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ABI424_RS03955 (ABI424_000793) cgtA 863541..864719 (-) 1179 WP_012340385.1 Obg family GTPase CgtA -
  ABI424_RS03960 (ABI424_000794) - 864729..865649 (-) 921 WP_012340377.1 DMT family transporter -
  ABI424_RS03965 (ABI424_000795) rpmA 865785..866042 (-) 258 WP_011608400.1 50S ribosomal protein L27 -
  ABI424_RS03970 (ABI424_000796) rplU 866063..866374 (-) 312 WP_011608399.1 50S ribosomal protein L21 -
  ABI424_RS03975 (ABI424_000797) ispB 866615..867586 (+) 972 WP_041604961.1 octaprenyl diphosphate synthase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15903.18 Da        Isoelectric Point: 8.4638

>NTDB_id=1004676 ABI424_RS03945 WP_012340401.1 862149..862607(-) (pilA) [Histophilus somni strain 23-038383_S91]
MKITYIFSSVKKGFTLIELMIVIAIVAILATIAIPSYQNYTKKAAITELLQASAPYRAEVELCVYNNDAPTNCNAGTNGI
QAAINTPKGKVKSVSVQGGAITVTGNGNLKDITYTLTPTGDASTGVTWSANCNNKADLFPAGFCSSTSTNSP

Nucleotide


Download         Length: 459 bp        

>NTDB_id=1004676 ABI424_RS03945 WP_012340401.1 862149..862607(-) (pilA) [Histophilus somni strain 23-038383_S91]
ATGAAAATAACCTATATTTTTTCATCAGTTAAAAAAGGATTTACTCTCATAGAACTGATGATTGTTATTGCTATTGTGGC
AATTCTTGCAACCATTGCAATTCCGTCATACCAAAATTATACAAAAAAAGCTGCAATTACAGAGCTATTGCAAGCTTCAG
CTCCTTATCGTGCAGAAGTTGAGCTATGCGTGTATAACAATGATGCACCGACAAATTGTAATGCTGGAACAAATGGAATT
CAAGCGGCAATCAATACACCAAAAGGTAAAGTAAAATCAGTTAGCGTGCAAGGTGGAGCCATTACTGTTACAGGGAATGG
AAATCTTAAAGATATTACTTATACCTTAACACCAACAGGCGATGCTTCAACCGGCGTTACTTGGAGTGCAAATTGCAATA
ATAAGGCGGATCTATTTCCTGCCGGATTTTGCTCTTCTACGTCTACAAACTCTCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-33)

Pilin (bacterial filament) (Pilin)

(43-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

65.306

96.711

0.632
  pilA Haemophilus influenzae Rd KW20

63.265

96.711

0.612
  pilA Glaesserella parasuis strain SC1401

50.323

100

0.513
  pilA Ralstonia pseudosolanacearum GMI1000

35.714

100

0.395
  pilA Vibrio campbellii strain DS40M4

38.356

96.053

0.368

Multiple sequence alignment