Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   EL327_RS08595 Genome accession   NZ_LR134342
Coordinates   1759934..1760473 (-) Length   179 a.a.
NCBI ID   WP_003116248.1    Uniprot ID   Q59651
Organism   Pseudomonas aeruginosa strain NCTC10728     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1754934..1765473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL327_RS08575 (NCTC10728_01680) - 1755618..1757873 (-) 2256 WP_016264271.1 DUF1631 domain-containing protein -
  EL327_RS08580 (NCTC10728_01681) nadC 1758159..1759007 (+) 849 WP_016562338.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EL327_RS08590 (NCTC10728_01683) - 1759172..1759870 (-) 699 WP_023122772.1 hypothetical protein -
  EL327_RS08595 pilE 1759934..1760473 (-) 540 WP_003116248.1 pilin Machinery gene
  EL327_RS08600 (NCTC10728_01684) pilB 1760702..1762405 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL327_RS08605 (NCTC10728_01685) pilC 1762409..1763626 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  EL327_RS08610 (NCTC10728_01686) pilD 1763629..1764501 (+) 873 WP_016562340.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  EL327_RS08615 (NCTC10728_01687) coaE 1764498..1765109 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  EL327_RS08620 (NCTC10728_01688) yacG 1765106..1765306 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18003.27 Da        Isoelectric Point: 4.6894

>NTDB_id=1002179 EL327_RS08595 WP_003116248.1 1759934..1760473(-) (pilE) [Pseudomonas aeruginosa strain NCTC10728]
MTAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVQIAEATGEITVTFNAANVGNIPANSTLVFTPYVQNAAGAPTQLGASYASGVTGSIDWGCASDSNAVSSGTDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1002179 EL327_RS08595 WP_003116248.1 1759934..1760473(-) (pilE) [Pseudomonas aeruginosa strain NCTC10728]
ATGACAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGCTTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACAAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCGCAAACGTGGGTAA
TATTCCGGCTAACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTTCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGGACTCCAACGCGGTTTCCAGTGGTACCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCTAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q59651

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.451

100

0.447
  pilA Ralstonia pseudosolanacearum GMI1000

44.444

100

0.447
  pilA2 Legionella pneumophila str. Paris

42.373

98.883

0.419
  pilA2 Legionella pneumophila strain ERS1305867

41.808

98.883

0.413
  comP Acinetobacter baylyi ADP1

38.889

100

0.391