Detailed information NTG1001329

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: EL139_RS02650
• Genome accession: NZ_LR134316
• Coordinates: 491381..491821 (-)
• Length: 146 a.a.
• NCBI ID: WP_111714778.1
• Uniprot ID: -
• Organism: Streptococcus dysgalactiae subsp. equisimilis strain NCTC6181
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 486381..496821

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL139_RS02620 (NCTC6181_00534)	-	487008..487445 (-)	438	WP_111714792.1	hypothetical protein	-
EL139_RS02625 (NCTC6181_00535)	-	487463..488143 (-)	681	WP_111714790.1	type II CAAX endopeptidase family protein	-
EL139_RS02630 (NCTC6181_00536)	-	488246..488452 (-)	207	WP_111714788.1	helix-turn-helix transcriptional regulator	-
EL139_RS02635 (NCTC6181_00537)	-	488758..489954 (-)	1197	WP_111714785.1	acetate kinase	-
EL139_RS02640 (NCTC6181_00538)	comGH	490013..490966 (-)	954	WP_111714783.1	class I SAM-dependent methyltransferase	Machinery gene
EL139_RS02645 (NCTC6181_00539)	comGG	491029..491403 (-)	375	WP_111714780.1	competence type IV pilus minor pilin ComGG	-
EL139_RS02650 (NCTC6181_00540)	comGF	491381..491821 (-)	441	WP_111714778.1	competence type IV pilus minor pilin ComGF	Machinery gene
EL139_RS02655 (NCTC6181_00541)	comGE	491802..492047 (-)	246	WP_231871990.1	competence type IV pilus minor pilin ComGE	Machinery gene
EL139_RS02660 (NCTC6181_00542)	comGD	492055..492483 (-)	429	WP_111714773.1	competence type IV pilus minor pilin ComGD	Machinery gene
EL139_RS02665 (NCTC6181_00543)	comGC	492458..492784 (-)	327	WP_111714771.1	competence type IV pilus major pilin ComGC	Machinery gene
EL139_RS02670 (NCTC6181_00544)	comGB	492786..493817 (-)	1032	WP_111717873.1	competence type IV pilus assembly protein ComGB	Machinery gene
EL139_RS02675 (NCTC6181_00545)	comGA/cglA	493753..494694 (-)	942	WP_111714768.1	competence type IV pilus ATPase ComGA	Machinery gene
EL139_RS02680 (NCTC6181_00546)	-	494775..495140 (-)	366	WP_003055257.1	DUF1033 family protein	-

Sequence

Protein

• Length: 146 a.a.
• Molecular weight: 16995.59 Da
• Isoelectric Point: 10.5554

>NTDB_id=1001329 EL139_RS02650 WP_111714778.1 491381..491821(-) (comGF) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC6181]
MKKRTGRVQAFTFLEALIALLVISGSLLVYQGLTKTLLSHSRYLTKNDQDHWLLFSQQLREELSGARFHKVENNKLYIEK
GKKKLVLGQFKSHDFRKSAGNGQGYQPMLFGLSHSYIQAEQSRIRITLHWKSGLERTFYYAFQDQP

Nucleotide

• Length: 441 bp

>NTDB_id=1001329 EL139_RS02650 WP_111714778.1 491381..491821(-) (comGF) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC6181]
TTGAAAAAAAGGACAGGTAGGGTCCAAGCATTTACTTTTTTAGAGGCTTTAATTGCCTTGCTGGTGATATCAGGTTCTCT
TCTAGTGTATCAAGGATTGACCAAAACACTCCTTAGCCACAGTCGCTACCTTACCAAAAATGATCAAGACCATTGGCTTT
TGTTCTCCCAGCAGTTACGAGAAGAGTTAAGTGGAGCGCGATTTCACAAAGTAGAGAACAATAAATTATATATCGAAAAA
GGCAAGAAAAAACTAGTTTTAGGACAATTTAAAAGCCATGACTTTCGAAAATCGGCAGGCAATGGACAAGGTTATCAACC
CATGCTGTTTGGCCTGTCTCATAGTTATATACAAGCAGAGCAGTCTCGTATTCGTATTACCCTTCACTGGAAAAGTGGAC
TAGAAAGGACCTTTTACTATGCGTTTCAAGACCAACCTTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (40-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Streptococcus mutans UA159	48.921	95.205	0.466
comGF	Streptococcus mutans UA140	48.201	95.205	0.459
comGF	Lactococcus lactis subsp. cremoris KW2	46.377	94.521	0.438
comGF/cglF	Streptococcus pneumoniae D39	43.796	93.836	0.411
comGF/cglF	Streptococcus mitis SK321	43.796	93.836	0.411
comGF/cglF	Streptococcus pneumoniae TIGR4	43.796	93.836	0.411
comGF/cglF	Streptococcus pneumoniae R6	43.796	93.836	0.411
comGF/cglF	Streptococcus mitis NCTC 12261	43.796	93.836	0.411
comGF/cglF	Streptococcus pneumoniae Rx1	43.796	93.836	0.411