Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFU29_RS16400 Genome accession   NZ_CP156001
Coordinates   3699315..3701048 (-) Length   577 a.a.
NCBI ID   WP_323539949.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain bglFP 6753     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3698034..3708481 3699315..3701048 within 0


Gene organization within MGE regions


Location: 3698034..3708481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU29_RS16390 (ABFU29_16365) - 3698034..3698396 (+) 363 WP_148263072.1 hypothetical protein -
  ABFU29_RS16395 (ABFU29_16370) - 3698341..3699132 (+) 792 WP_407462317.1 toxin -
  ABFU29_RS16400 (ABFU29_16375) pilB 3699315..3701048 (-) 1734 WP_323539949.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU29_RS16405 (ABFU29_16380) - 3701083..3702609 (-) 1527 WP_323541664.1 hypothetical protein -
  ABFU29_RS16410 (ABFU29_16385) - 3702579..3703208 (-) 630 WP_323548213.1 hypothetical protein -
  ABFU29_RS16415 (ABFU29_16390) - 3703219..3704730 (-) 1512 WP_407463088.1 phosphoethanolamine transferase -
  ABFU29_RS16420 (ABFU29_16395) comP 3704949..3705362 (-) 414 WP_323548210.1 pilin Machinery gene
  ABFU29_RS16425 (ABFU29_16400) pilC 3705715..3706974 (+) 1260 WP_323548209.1 type II secretion system F family protein Machinery gene
  ABFU29_RS16430 (ABFU29_16405) - 3706981..3707844 (+) 864 WP_323548208.1 A24 family peptidase -
  ABFU29_RS16435 (ABFU29_16410) coaE 3707858..3708481 (+) 624 WP_076038488.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62693.87 Da        Isoelectric Point: 5.7669

>NTDB_id=1001016 ABFU29_RS16400 WP_323539949.1 3699315..3701048(-) (pilB) [Xanthomonas campestris pv. raphani strain bglFP 6753]
MNSVATTNLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFSEKKLVSASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTKALDDIKFHTNLVVEPILVDEDQIRRTLEQWQAGNASFGSALG
GDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1001016 ABFU29_RS16400 WP_323539949.1 3699315..3701048(-) (pilB) [Xanthomonas campestris pv. raphani strain bglFP 6753]
ATGAATTCAGTAGCTACCACCAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AGCCGCTGCGCGGACGGCGATGGAGCAGGCGGCCGCTGCCAAAGTGCCGCTGCCGCAGTGGTTTTCCGAGAAGAAGCTGG
TGTCGGCGTCGCAGCTGGCGGCAGCCAACGCCGTGGAATTCGGTATGCCGCTGATGGATGTGTCGGTGTTCGACGCTAGT
CAGAACGCGGTGAAGCTGGTAAGTGAAGAGTTGCTCCAGAAGCATCAGGTGTTGCCGCTGTTCAAGCGCGGCAACCGATT
GTTTGTGGGGGTGAGCAACCCGACGCAGACCAAGGCATTGGACGACATCAAGTTCCATACCAACTTGGTGGTGGAGCCAA
TCCTGGTCGATGAGGACCAGATCCGTAGGACGTTGGAGCAATGGCAGGCAGGAAACGCGTCCTTTGGCTCGGCGCTTGGT
GGCGACGACGAGGAGATGGGGGACCTAGACGTGTCCGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGTGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGATA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCTTGAAGGTAATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTCGGGATTCTCAACGACGAGACACGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCCG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCGGCGGCGTTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTTGAGACGGCCGAGATTGCGATCAAGGCAGCGCAGACGGGCCACATGGT
GCTGTCGACACTGCACACCAACGATGCGCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATCA
CCTCGTCGGTGACCCTGGTCATCGCGCAGCGCTTGGCACGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAGGCGGTCGGTTGCGATGA
ATGCACGGAAGGCTACAAGGGCCGCACCGGTATCTATCAGGTGATGCCGATGACGGACGAAATCGGTGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGCGTGACCAGCCTGGCCGAAATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.69

98.44

0.558
  pilB Acinetobacter baylyi ADP1

55.652

99.653

0.555
  pilB Legionella pneumophila strain ERS1305867

52.465

98.44

0.516
  pilB Vibrio cholerae strain A1552

49.567

100

0.496
  pilF Neisseria gonorrhoeae MS11

49.296

98.44

0.485
  pilB Vibrio campbellii strain DS40M4

46.046

98.614

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

46.512

96.88

0.451

Multiple sequence alignment