Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFU21_RS16455 Genome accession   NZ_CP155988
Coordinates   3764613..3766346 (-) Length   577 a.a.
NCBI ID   WP_075287148.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain LMG 08007     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3759613..3771346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU21_RS16440 (ABFU21_16440) - 3760981..3762591 (+) 1611 WP_011038210.1 PAS domain-containing sensor histidine kinase -
  ABFU21_RS16445 (ABFU21_16445) pilR 3762800..3764194 (+) 1395 WP_043877723.1 sigma-54 dependent transcriptional regulator Regulator
  ABFU21_RS16455 (ABFU21_16455) pilB 3764613..3766346 (-) 1734 WP_075287148.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU21_RS16460 (ABFU21_16460) - 3766411..3766812 (-) 402 WP_194734302.1 pilin -
  ABFU21_RS16465 (ABFU21_16465) pilE 3766925..3767365 (-) 441 WP_011038214.1 pilin Machinery gene
  ABFU21_RS16470 (ABFU21_16470) pilC 3767692..3768948 (+) 1257 WP_011038215.1 type II secretion system F family protein Machinery gene
  ABFU21_RS16475 (ABFU21_16475) - 3768955..3769818 (+) 864 WP_011038216.1 A24 family peptidase -
  ABFU21_RS16480 (ABFU21_16480) coaE 3769832..3770455 (+) 624 WP_011038217.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62757.01 Da        Isoelectric Point: 5.5903

>NTDB_id=1000803 ABFU21_RS16455 WP_075287148.1 3764613..3766346(-) (pilB) [Xanthomonas campestris pv. campestris strain LMG 08007]
MSVVLTANLVGITGIARRLVQDGALEEAVARGAMDQAALAKVPLPQWFAEKKLVSAAQLASANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLG
DDDDEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAAGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1000803 ABFU21_RS16455 WP_075287148.1 3764613..3766346(-) (pilB) [Xanthomonas campestris pv. campestris strain LMG 08007]
ATGAGCGTTGTGCTAACGGCTAATCTTGTTGGAATCACGGGCATTGCTCGACGTCTTGTGCAAGACGGTGCGCTCGAAGA
GGCGGTTGCTCGTGGTGCGATGGATCAGGCAGCACTCGCCAAAGTGCCTTTACCCCAGTGGTTTGCTGAAAAAAAGCTAG
TCTCTGCTGCGCAGCTCGCCTCTGCCAATGCAGTTGAGTTCGGTATGCCGCTGATGGATGTGTCGGTGTTCGACGCCAGC
CAGAACGCGGTCAAGCTGGTCAGTGAGGAGTTGCTTCAGAAGCATCAGGTACTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAATCCGACGCAGACCAGGGCGCTGGACGATATCAAGTTCCACACCAATCTGGTGGTCGAGCCGA
TCCTGGTAGACGAAGACCAGATCCGTCGCACATTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGCTCTTCGCTCGGC
GACGACGATGATGAGATGGGGGATCTGGACGTCTCTACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCAACCCTGTTCGGCGAGA
AGGTGGTACTGCGTATCCTGGACGCCAGTGCGGCCAAGCTTGGTATCGAGAAGCTGGGCTATGAGGCGGACCAACAGAAG
CTGTTCCTGGATGCGATACACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTCGGGATTCTCAACGACGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTTGAAATCCGCTTGCCTG
GTGTGAATCAGGTGCAGCAGAACGTCAAGCGCGGTATGACCTTCGCGGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
TCTGTCGACACTGCACACCAACGATGCGCCGCAAACCATTGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCTCGTCGGTGACTCTGGTCATCGCGCAGCGCTTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGCCGGTTGCGATGA
GTGCACGGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGACGGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCACAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGGGTGACCAGCCTGGCCGAAATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.44

0.551
  pilB Acinetobacter baylyi ADP1

55.282

98.44

0.544
  pilB Legionella pneumophila strain ERS1305867

51.761

98.44

0.51
  pilF Neisseria gonorrhoeae MS11

48.239

98.44

0.475
  pilB Vibrio cholerae strain A1552

49.183

95.494

0.47
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

45.471

97.574

0.444

Multiple sequence alignment