Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFT91_RS05235 Genome accession   NZ_CP155960
Coordinates   1242092..1243825 (+) Length   577 a.a.
NCBI ID   WP_323539949.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain NBC567     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1237092..1248825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFT91_RS05215 (ABFT91_05210) pilA2 1237772..1238197 (+) 426 WP_323539945.1 pilin Machinery gene
  ABFT91_RS05220 (ABFT91_05215) - 1238332..1239921 (+) 1590 WP_323539946.1 phosphoethanolamine transferase -
  ABFT91_RS05225 (ABFT91_05220) - 1239935..1240561 (+) 627 WP_323539947.1 hypothetical protein -
  ABFT91_RS05230 (ABFT91_05225) - 1240531..1242057 (+) 1527 WP_323539948.1 hypothetical protein -
  ABFT91_RS05235 (ABFT91_05230) pilB 1242092..1243825 (+) 1734 WP_323539949.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFT91_RS05240 (ABFT91_05235) pilR 1244239..1245633 (-) 1395 WP_323530785.1 sigma-54 dependent transcriptional regulator Regulator
  ABFT91_RS05245 (ABFT91_05240) - 1245842..1247452 (-) 1611 WP_014508702.1 PAS domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62693.87 Da        Isoelectric Point: 5.7669

>NTDB_id=1000301 ABFT91_RS05235 WP_323539949.1 1242092..1243825(+) (pilB) [Xanthomonas campestris pv. raphani strain NBC567]
MNSVATTNLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFSEKKLVSASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTKALDDIKFHTNLVVEPILVDEDQIRRTLEQWQAGNASFGSALG
GDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1000301 ABFT91_RS05235 WP_323539949.1 1242092..1243825(+) (pilB) [Xanthomonas campestris pv. raphani strain NBC567]
ATGAATTCAGTAGCTACCACCAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AGCCGCTGCGCGGACGGCGATGGAGCAGGCGGCCGCTGCCAAAGTGCCGCTGCCGCAGTGGTTTTCCGAGAAGAAGCTGG
TGTCGGCGTCGCAGCTGGCGGCAGCCAACGCCGTGGAATTCGGTATGCCGCTGATGGATGTGTCGGTGTTCGACGCTAGT
CAGAACGCGGTGAAGCTGGTAAGTGAAGAGTTGCTCCAGAAGCATCAGGTGTTGCCGCTGTTCAAGCGCGGCAACCGATT
GTTTGTGGGGGTGAGCAACCCGACGCAGACCAAGGCATTGGACGACATCAAGTTCCATACCAACTTGGTGGTGGAGCCAA
TCCTGGTCGATGAGGACCAGATCCGTAGGACGTTGGAGCAATGGCAGGCAGGAAACGCGTCCTTTGGCTCGGCGCTTGGT
GGCGACGACGAGGAGATGGGGGACCTAGACGTGTCCGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGTGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGATA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCTTGAAGGTAATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGATTTCCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGTTGCGTATCCTGGACGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGTTACGAGGCGGATCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTCGGGATTCTCAACGACGAGACCCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCCG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCACATGGT
GCTGTCGACACTGCACACCAACGATGCGCCCCAGACCATCGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATTA
CCTCATCGGTGACCCTGGTCATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
ATGCACGGAAGGCTACAAAGGCCGTACCGGCATCTATCAGGTGATGCCGATGACGGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTAATGAAG
GCTTCGCACGGCGTGACCAGCCTGGCCGAGATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.69

98.44

0.558
  pilB Acinetobacter baylyi ADP1

55.652

99.653

0.555
  pilB Legionella pneumophila strain ERS1305867

52.465

98.44

0.516
  pilB Vibrio cholerae strain A1552

49.567

100

0.496
  pilF Neisseria gonorrhoeae MS11

49.296

98.44

0.485
  pilB Vibrio campbellii strain DS40M4

46.046

98.614

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

46.512

96.88

0.451

Multiple sequence alignment