Detailed information NTG0999983

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: EL070_RS00345
• Genome accession: NZ_LR134284
• Coordinates: 55414..56412 (+)
• Length: 332 a.a.
• NCBI ID: WP_002986679.1
• Uniprot ID: A2RC05
• Organism: Streptococcus pyogenes strain NCTC8232
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 50414..61412

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL070_RS00330 (NCTC8232_00065)	-	51411..52829 (+)	1419	WP_011888524.1	hypothetical protein	-
EL070_RS00335 (NCTC8232_00066)	purB	52849..54141 (+)	1293	WP_111705416.1	adenylosuccinate lyase	-
EL070_RS00340 (NCTC8232_00067)	comR	54276..55187 (+)	912	WP_002986681.1	transcriptional regulator Rgg4/ComR	Regulator
EL070_RS10455 (NCTC8232_00068)	comS	55272..55367 (+)	96	WP_198462994.1	quorum-sensing system DWW-type pheromone	Regulator
EL070_RS00345 (NCTC8232_00069)	ruvB	55414..56412 (+)	999	WP_002986679.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
EL070_RS00350 (NCTC8232_00070)	-	56550..56987 (+)	438	WP_002994668.1	low molecular weight protein-tyrosine-phosphatase	-
EL070_RS00355 (NCTC8232_00071)	-	57010..57411 (+)	402	WP_011017240.1	MORN repeat-containing protein	-
EL070_RS00360 (NCTC8232_00072)	-	57408..59183 (+)	1776	WP_111705417.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37565.11 Da
• Isoelectric Point: 5.3005

>NTDB_id=999983 EL070_RS00345 WP_002986679.1 55414..56412(+) (ruvB) [Streptococcus pyogenes strain NCTC8232]
MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=999983 EL070_RS00345 WP_002986679.1 55414..56412(+) (ruvB) [Streptococcus pyogenes strain NCTC8232]
ATGGCTAGAATTTTAGACAATAATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCGGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTCGAGGAAGTACTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGATTATGCTCAAATTATTGGTGATGGCATTATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAGAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGGCCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACTCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: A2RC05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae D39	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae TIGR4	84.545	99.398	0.84
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473