Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFU06_RS05475 Genome accession   NZ_CP155938
Coordinates   1297008..1298714 (+) Length   568 a.a.
NCBI ID   WP_228943908.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain Xcc-R1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1292008..1303714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU06_RS05445 (ABFU06_05425) - 1292462..1292717 (-) 256 Protein_1058 SymE family type I addiction module toxin -
  ABFU06_RS05450 (ABFU06_05430) coaE 1292911..1293534 (-) 624 WP_225042504.1 dephospho-CoA kinase -
  ABFU06_RS05455 (ABFU06_05435) - 1293548..1294411 (-) 864 WP_011038216.1 A24 family peptidase -
  ABFU06_RS05460 (ABFU06_05440) pilC 1294418..1295674 (-) 1257 WP_075285409.1 type II secretion system F family protein Machinery gene
  ABFU06_RS05465 (ABFU06_05445) - 1296011..1296460 (+) 450 WP_228943907.1 pilin -
  ABFU06_RS05470 (ABFU06_05450) - 1296575..1296949 (+) 375 WP_269467167.1 pilin -
  ABFU06_RS05475 (ABFU06_05455) pilB 1297008..1298714 (+) 1707 WP_228943908.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU06_RS05485 (ABFU06_05465) pilR 1300338..1301732 (-) 1395 WP_228422808.1 sigma-54 dependent transcriptional regulator Regulator
  ABFU06_RS05490 (ABFU06_05470) - 1301941..1303551 (-) 1611 WP_076037049.1 PAS domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 61777.84 Da        Isoelectric Point: 5.7640

>NTDB_id=999930 ABFU06_RS05475 WP_228943908.1 1297008..1298714(+) (pilB) [Xanthomonas campestris pv. raphani strain Xcc-R1]
MGITGIARRLVQDGSLEEGAARSAMDQAAAAKIPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSVFDASQNAVKLVSE
ELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMGDDDEGMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQKLFLDAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPEHALLAEGF
TPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMKASHGVTSLA
EINRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=999930 ABFU06_RS05475 WP_228943908.1 1297008..1298714(+) (pilB) [Xanthomonas campestris pv. raphani strain Xcc-R1]
ATCGGAATAACTGGAATAGCCCGTCGTTTGGTACAGGACGGCTCATTGGAAGAAGGCGCTGCGCGTTCAGCAATGGACCA
GGCTGCTGCCGCAAAAATTCCACTGCCTCAGTGGTTTGCTGAAAAAAAATTAGTATCTGCAGCTCAGCTCGCCGCAGCTA
ATGCCGTAGAGTTCGGTATGCCGCTGATGGATGTGTCGGTGTTTGACGCCAGCCAGAACGCGGTCAAGTTGGTCAGTGAG
GAGTTGCTCCAGAAGCATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGTTTGTTTGTGGGGGTGAGCAACCCGACCCA
GACCCGTGCGCTGGACGACATCAAGTTCCATACTAATCTTGTGGTCGAGCCGATCCTCGTCGACGAGGATCAGATCCGGC
GCACCTTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGTTCGTCGATGGGGGACGACGACGAGGGGATGGGAGACCTA
GACGTGTCCGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCGAAGGGCGATGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACATCCATTTCGAGCCGTATGAAGATGACT
ACCGGGTGCGGTTGCGGATTGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCGGTGAAGCTCAACCAGCGCATCGCGGCG
CGCTTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAAGACGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGATTTTCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAGAAGGTGGTGCTGCGTATCCTGGACGCCA
GCGCGGCCAAGCTCGGTATCGAGAAGCTGGGTTACGAGGCGGATCAGCAGAAGCTGTTCCTGGATGCGATCCACAAGCCC
TACGGCATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACCGTGTCGCTGTATACCGCGCTCGGGATTCTCAACGA
CGAGACCCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCCGGCGTCAATCAGGTGCAGCAGAACGTCA
AGCGGGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCGGACATCATCATGGTCGGCGAAATCCGC
GACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCACATGGTGCTGTCGACACTGCACACCAACGATGC
GCCGCAAACCATTGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCACCTCGTCGGTGACCCTGGTCATCGCGC
AGCGTCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCGGAACATGCGCTGCTGGCGGAAGGCTTT
ACGCCGGAGCAGGTTGCGAACATCGAGCTGTATGAAGCGGTCGGTTGCGATGAATGCACGGAAGGCTACAAGGGCCGTAC
CGGCATCTATCAGGTGATGCCGATGACCGATGAAATCGGCGCGATCGTGCTGGAAGGCGGCAATGCGATGCAGATCGCCG
AGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGTCAGTCGGCATTGATGAAGGCTTCGCACGGCGTGACTAGCCTGGCC
GAGATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-144)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

100

0.562
  pilB Acinetobacter baylyi ADP1

55.458

100

0.555
  pilB Legionella pneumophila strain ERS1305867

52.641

100

0.526
  pilF Neisseria gonorrhoeae MS11

48.592

100

0.486
  pilB Vibrio cholerae strain A1552

49.548

97.359

0.482
  pilB Vibrio campbellii strain DS40M4

46.397

100

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

46.691

98.415

0.46

Multiple sequence alignment