Detailed information NTG0999859

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ABFU36_RS05545
• Genome accession: NZ_CP155933
• Coordinates: 1304729..1306462 (+)
• Length: 577 a.a.
• NCBI ID: WP_011038212.1
• Uniprot ID: Q8P673
• Organism: Xanthomonas campestris pv. campestris strain CFBP 1124
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	1303019..1304356	1304729..1306462	flank	373

Gene organization within MGE regions

Location: 1303019..1306462

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABFU36_RS05535 (ABFU36_05520)	-	1303019..1304386 (-)	1368	WP_011035783.1	IS5-like element IS1478 family transposase	-
ABFU36_RS05540 (ABFU36_05525)	-	1304566..1304664 (+)	99	WP_407366569.1	hypothetical protein	-
ABFU36_RS05545 (ABFU36_05530)	pilB	1304729..1306462 (+)	1734	WP_011038212.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 577 a.a.
• Molecular weight: 62785.06 Da
• Isoelectric Point: 5.5903

>NTDB_id=999859 ABFU36_RS05545 WP_011038212.1 1304729..1306462(+) (pilB) [Xanthomonas campestris pv. campestris strain CFBP 1124]
MSVVLTANLVGITGIARRLVQDGALEEAVARGAMDQAALAKVPLPQWFAEKKLVSAAQLASANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLG
DDDDEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide

• Length: 1734 bp

>NTDB_id=999859 ABFU36_RS05545 WP_011038212.1 1304729..1306462(+) (pilB) [Xanthomonas campestris pv. campestris strain CFBP 1124]
ATGAGCGTTGTGCTAACGGCTAATCTTGTTGGAATCACGGGCATTGCTCGACGTCTTGTGCAAGACGGTGCGCTCGAAGA
GGCGGTTGCTCGTGGTGCGATGGATCAGGCAGCACTCGCCAAAGTGCCTTTACCCCAGTGGTTTGCTGAAAAAAAGCTAG
TCTCTGCTGCGCAGCTCGCCTCTGCCAATGCAGTTGAGTTCGGTATGCCGCTGATGGATGTGTCGGTGTTCGACGCCAGC
CAGAACGCGGTCAAGCTGGTCAGTGAGGAGTTGCTTCAGAAGCATCAGGTACTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAATCCGACGCAGACCAGGGCGCTGGACGATATCAAGTTCCACACCAATCTGGTGGTCGAGCCGA
TCCTGGTAGACGAAGACCAGATCCGTCGCACATTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTCGGCTCTTCGCTCGGC
GACGACGATGATGAGATGGGGGATCTGGACGTCTCTACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCAACCCTGTTCGGCGAGA
AGGTGGTACTGCGTATCCTGGACGCCAGTGCGGCCAAGCTTGGTATCGAGAAGCTGGGCTATGAGGCGGACCAACAGAAG
CTGTTCCTGGATGCGATACACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTATACCGCGCTCGGGATTCTCAACGACGAGACGCGCAATATTTCCACGGCCGAGGATCCGGTTGAAATCCGCTTGCCTG
GTGTGAATCAGGTGCAGCAGAACGTCAAGCGCGGTATGACCTTCGCGGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
TCTGTCGACACTGCACACCAACGATGCGCCGCAAACCATTGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCTCGTCGGTGACTCTGGTCATCGCGCAGCGCTTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
GTGCACGGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGACGGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCACAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGGGTGACCAGCCTGGCCGAAATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (201-468)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-153)

3D structure

• AlphaFold DB: Q8P673

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	56.162	98.44	0.553
pilB	Acinetobacter baylyi ADP1	55.458	98.44	0.546
pilB	Legionella pneumophila strain ERS1305867	51.937	98.44	0.511
pilF	Neisseria gonorrhoeae MS11	48.415	98.44	0.477
pilB	Vibrio cholerae strain A1552	49.002	95.494	0.468
pilB	Vibrio campbellii strain DS40M4	45.694	98.614	0.451
pilB	Vibrio parahaemolyticus RIMD 2210633	45.471	97.574	0.444