Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ABFB49_RS25860 Genome accession   NZ_CP155726
Coordinates   5603042..5605186 (-) Length   714 a.a.
NCBI ID   WP_014603377.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2024CK-00357     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5598042..5610186
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFB49_RS25845 (ABFB49_25845) - 5599700..5601349 (-) 1650 WP_114230873.1 AAA family ATPase -
  ABFB49_RS25850 (ABFB49_25850) aroB 5601356..5602462 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  ABFB49_RS25855 (ABFB49_25855) aroK 5602512..5603030 (-) 519 WP_003095833.1 shikimate kinase AroK -
  ABFB49_RS25860 (ABFB49_25860) pilQ 5603042..5605186 (-) 2145 WP_014603377.1 type 4a pilus secretin PilQ Machinery gene
  ABFB49_RS25865 (ABFB49_25865) pilP 5605240..5605764 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  ABFB49_RS25870 (ABFB49_25870) pilO 5605761..5606384 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  ABFB49_RS25875 (ABFB49_25875) pilN 5606381..5606977 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  ABFB49_RS25880 (ABFB49_25880) pilM 5606977..5608041 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77325.86 Da        Isoelectric Point: 5.2219

>NTDB_id=998805 ABFB49_RS25860 WP_014603377.1 5603042..5605186(-) (pilQ) [Pseudomonas aeruginosa strain 2024CK-00357]
MNSGLSRLGIALLAAMLAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASTAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
NGYGKDGNIGIKDEDGMNCGPIAGNCTFPTTGTSKSPSPFVDIGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDFDRALNGVPPINKN
EVNAKILVNDGETIVIGGVFSNTQSKSVDKVPFLGDLPYLGRLFRRDTVSDVKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=998805 ABFB49_RS25860 WP_014603377.1 5603042..5605186(-) (pilQ) [Pseudomonas aeruginosa strain 2024CK-00357]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTAGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCATTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTACAGAACAAGCTCGGCACCAAGAATCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCACGTT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCACCGCTCCGGTCAAGGCTAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCATACGTGCCGGCGGGT
CGTGCCATTCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGTGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGTTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTATCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACGGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTCCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGATGGTGGCCAGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGCATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAACTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CACGCATCGTCGAGGCCAACGTCGGTTACGACAAGAGCCTCGGTGTGCGCTGGGGCGGCGCTTATCACAAGGGTAACTGG
AACGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGCAACTGTAC
TTTCCCCACCACGGGAACCAGCAAGTCGCCGTCGCCGTTCGTCGACATCGGCGCCAAGGATGCCACCTCCGGGATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCCAGGAAGTACCCTACCAGGAGGCCAGCTC
CAGTGGCGCTACCAGCACCTCGTTCAAGGAAGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACCGGA
TCATCGTCGAAGTGAAGGTGACCAAGGATGCGCCGGACTTCGATCGCGCACTGAACGGCGTACCGCCGATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACGACGGCGAGACCATCGTCATCGGCGGCGTGTTCAGCAACACCCAGAGCAAGTC
GGTGGACAAGGTGCCGTTCCTCGGCGACCTGCCTTATCTGGGGCGGCTGTTCCGGCGCGATACGGTCTCGGACGTGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)

Secretin and TonB N terminus short domain (STN)

(307-353)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(549-705)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

98.039

100

0.98
  pilQ Acinetobacter baumannii strain A118

40.054

100

0.417
  pilQ Acinetobacter baumannii D1279779

40.054

100

0.417
  comQ Acinetobacter baylyi ADP1

41.738

98.319

0.41

Multiple sequence alignment