Detailed information NTG0998274

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABEG12_RS02290
• Genome accession: NZ_CP155639
• Coordinates: 466882..468030 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain TY922
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 461882..473030

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABEG12_RS02265 (ABEG12_02265)	-	463033..463329 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ABEG12_RS02270 (ABEG12_02270)	-	463326..463919 (-)	594	WP_003084544.1	YggT family protein	-
ABEG12_RS02275 (ABEG12_02275)	proC	463930..464751 (-)	822	WP_023092309.1	pyrroline-5-carboxylate reductase	-
ABEG12_RS02280 (ABEG12_02280)	-	464763..465455 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABEG12_RS02285 (ABEG12_02285)	pilT	465670..466704 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
ABEG12_RS02290 (ABEG12_02290)	pilU	466882..468030 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
ABEG12_RS02295 (ABEG12_02295)	czcD	468038..468937 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ABEG12_RS02300 (ABEG12_02300)	-	469092..469496 (+)	405	WP_003084560.1	NINE protein	-
ABEG12_RS02305 (ABEG12_02305)	-	469739..471112 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
ABEG12_RS02310 (ABEG12_02310)	-	471109..472293 (+)	1185	WP_023092310.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=998274 ABEG12_RS02290 WP_003110016.1 466882..468030(+) (pilU) [Pseudomonas aeruginosa strain TY922]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=998274 ABEG12_RS02290 WP_003110016.1 466882..468030(+) (pilU) [Pseudomonas aeruginosa strain TY922]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382