Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ABDZ23_RS16865 Genome accession   NZ_CP155621
Coordinates   3729185..3730249 (+) Length   354 a.a.
NCBI ID   WP_096794691.1    Uniprot ID   -
Organism   Pseudomonas koreensis strain BM06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3724185..3735249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABDZ23_RS16855 (ABDZ23_16855) - 3725008..3726276 (+) 1269 WP_003220889.1 malic enzyme-like NAD(P)-binding protein -
  ABDZ23_RS16860 (ABDZ23_16860) - 3726521..3728968 (-) 2448 WP_183783789.1 penicillin-binding protein 1A -
  ABDZ23_RS16865 (ABDZ23_16865) pilM 3729185..3730249 (+) 1065 WP_096794691.1 pilus assembly protein PilM Machinery gene
  ABDZ23_RS16870 (ABDZ23_16870) - 3730249..3730815 (+) 567 WP_027611312.1 PilN domain-containing protein -
  ABDZ23_RS16875 (ABDZ23_16875) pilO 3730812..3731435 (+) 624 WP_247269350.1 type 4a pilus biogenesis protein PilO -
  ABDZ23_RS16880 (ABDZ23_16880) - 3731432..3731959 (+) 528 WP_247269349.1 pilus assembly protein PilP -
  ABDZ23_RS16885 (ABDZ23_16885) pilQ 3731973..3734051 (+) 2079 WP_247269348.1 type IV pilus secretin PilQ Machinery gene
  ABDZ23_RS16890 (ABDZ23_16890) aroK 3734056..3734574 (+) 519 WP_027611316.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38104.68 Da        Isoelectric Point: 4.9026

>NTDB_id=998099 ABDZ23_RS16865 WP_096794691.1 3729185..3730249(+) (pilM) [Pseudomonas koreensis strain BM06]
MLGLFKKKTNTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPPNAVVEKNIAELEGVGHALSRVLVKARTGLKSVAV
AVAGSAVITKIIEMDAGLTDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVSVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=998099 ABDZ23_RS16865 WP_096794691.1 3729185..3730249(+) (pilM) [Pseudomonas koreensis strain BM06]
GTGCTGGGACTCTTCAAGAAAAAGACCAATACGTTACTGGGGATCGACATCAGCTCCACTTCGGTGAAGCTGCTGGAGTT
GAGCCGTCAGGGCGACCGCTACCGGGTCGAGGCCTACGCGGTAGAGCCGCTGCCGCCCAACGCCGTGGTCGAAAAGAATA
TCGCCGAGCTCGAAGGCGTGGGCCACGCCTTGTCCCGGGTGCTGGTCAAGGCACGCACCGGGCTCAAGAGCGTGGCGGTG
GCGGTGGCCGGTTCTGCGGTGATCACCAAGATCATCGAGATGGACGCCGGGCTGACCGATGACGAGCTGGAAAACCAGCT
CAAGATCGAAGCCGACCAATACATTCCCTATCCACTGGACGAAGTCGCCATCGATTTCGAAGTCCAGGGCGTGTCGCCGC
GCAACCCGGAGCGGGTCAGCGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTGGCGCTG
GCCGGGCTGACCGCGCGCGTGGTCGATGTCGAGGCCTATGCGCTAGAGCGCTCGTTCGGTCTGCTCGCCACTCAACTGGC
AGCCTCGCAGGAACGCCTGACCGTGGCGGTGGTGGACATCGGCGCGACCATGACTACCCTCAGCGTCCTGCATAACGGCC
GCATCATCTATACCCGCGAGCAACTGTTCGGTGGCCGGCAACTGACCGAGGAAATCCAGCGCCGCTACGGCCTGACTGTC
GAGCAGGCCGGGCTGGCGAAAAAGCAGGGCGGTCTGCCGGACGACTATGTCAGCGAGGTATTGCAACCGTTCCGTGATGC
TCTGGTGCAGCAGGTCTCGCGCTCGTTGCAGTTCTTCTTTGCCTCCGGGCAGTACAACGCCGTCGATCACATCCTGTTGG
CCGGCGGCACCGCCTCGGTGCCGGGGCTGGATCGGCTGATCGAACAGCGTCTGGGCACCCCGACCCAGGTCGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCCAGTGACGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

55.367

100

0.554
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486

Multiple sequence alignment